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The map label for this gene is uvrD [H]

Identifier: 157368435

GI number: 157368435

Start: 209133

End: 211304

Strand: Direct

Name: uvrD [H]

Synonym: Spro_0186

Alternate gene names: 157368435

Gene position: 209133-211304 (Clockwise)

Preceding gene: 157368434

Following gene: 157368437

Centisome position: 3.84

GC content: 59.58

Gene sequence:

>2172_bases
ATGGTGCCTATGGACGTTTCCGATCTGCTCGACAGCCTGAATGAAAAACAACGCGAAGCGGTGGCGGCGCCACGTGGCAA
CCTGTTGGTACTGGCCGGCGCGGGCAGCGGTAAAACCCGGGTGCTGGTGCATCGCATCGCCTGGCTGCTGGCGGTAGAGA
ACTGCTCGCCGTATTCCATTATGGCGGTGACCTTCACCAACAAGGCGGCGGCGGAAATGCGCCACCGTATCGAACACCTG
ATTGGCACCAGCCAGGGCGGCATGTGGATTGGTACCTTCCACGGCCTGGCTCATCGGCTGCTGCGCGCTCATCATTTGGA
TGCCAACCTGCCGCAGGATTTCCAAATTCTTGACAGCGACGACCAGTTCCGCCTGCTCAAGCGTATCGTCAAAGCGCTGA
ATATCGACGAGAAGCAATGGCCGCCGCGTCAGGCAATGTGGTACATCAACGGCAAAAAAGATGAGGGCCTGCGCCCGCAG
CACGTTGAAACCTACAACAACCCGGTGGAAGCGACCTGGCTGCGTATCTACCAGGCCTACCAGGAAGCCTGCGATCGCGC
CGGGCTGGTGGACTTTGCCGAGCTGTTGCTGCGTGCCCATGAGCTGTGGCTGAACAAGCCGCATATCCTCAATCATTACC
GCGAACGCTTCACCAACGTGCTGGTGGACGAGTTTCAGGATACCAACAGCATTCAGTACGCCTGGATCCGCCTGCTGGCC
GGCAATAACAGCAACGTGATGATCGTCGGCGACGATGACCAGTCGATCTACGGTTGGCGCGGCGCTCAGGTGGAAAATAT
CCAACGCTTCCTGAAGGATTTCCCCGGTGCGCAAAGCGTACTTCTGGAACAGAACTACCGTTCGACCAGCAATATCCTGA
AATCGGCCAATACCCTGATCGCCAACAACGGCGGGCGCATGGGGAAAAATCTGTGGACCGAAGGCGGCGAAGGCGAGCCT
ATCTCAGTCTACTGTGCCTTCAACGAGCTGGATGAATCGCGCTTCGTAGTGGGCCGTATCAAAGCCTGGCAGGAAAACGG
CGGTGCGCTGAATGATTGCGCCATCCTTTATCGCAGCAATGCCCAGTCGCGCGTACTGGAAGAGGCGCTGCTGCAAACGG
CGATGCCATACCGCATTTACGGCGGGCAACGCTTCTTCGAGCGTCAGGAGATCAAAGATGCCCTGGCCTACCTGCGGCTG
ATAGCCAACCGCAACGACGATGCTGCCTTCGAACGCGTGGTGAATACACCGACGCGCGGCATTGGCGACCGGACGCTGGA
CGAGGTGCGCCGCACGGCCCGCGATCGTCAATTGACCATGTGGCAGGCCACCCGCGTGCTGCTACAGGACAAAGTGCTGG
CCGGGCGTGCGGCATCCGCGCTGCAACGCTTTACCGAACTGTTGGACTCACTGGCGCACGAAACCGCCGATATGCCACTG
CACGTTCAGACCGATCGGGTGATCCGCGATTCGGGGCTGTTCATTATGTATGAGCAGGAGAAGGGCGAAAAAGGTCAGGC
ACGTATCGAAAACCTTGAGGAACTGGTGACGGCAACGCGCCAGTACAGCTATCAGGATGAAGATCAGGATCTGATGCCGC
TGCAGGCGTTTCTGTCACATGCTGCGCTGGAAGCGGGCGAGGGGCAGGCCGATGCCTATCAGGACGCGGTGCAGTTGATG
ACCCTGCACTCGGCCAAGGGGCTGGAGTTCCCGCAGGTGTTTATCGTCGGCATGGAAGAGGGGATGTTCCCGAGCCAAAT
GTCGCTGGACGAAGGCGGCAGGCTGGAAGAAGAGCGCCGTCTGGCCTACGTTGGGTTGACCCGCGCCATGCAGAAACTGA
CGCTGACCTATGCCGAAACCCGGCGTCTGTACGGCAAAGAGGTGTATCACCGGCCTTCACGTTTCATCGGCGAACTGCCG
GAGGAGTGTGTGGAAGAAGTGCGTTTGCGCGCCAGCGTCTCACGGCCGGCGAATCATCGCCGCATGGGCACGCCGATCAG
CGAAAACGACACCGGTTACAAGCTGGGCCAGCGAGTGCGCCACCCCAAGTTTGGCGAAGGGACTATCGTCAATCTGGAAG
GCAGCGGCGAACACAGCCGGCTGCAGATTGCTTTTCCGGGTGAAGGCATCAAGTGGCTGGTGGCCGCTTACGCCCGTCTG
GAGACGGTGTAA

Upstream 100 bases:

>100_bases
CGCAGTTGGCATCGCTGACAGCATTGTTATAATCCCTGCCAGAACTCTGTATAAATTCCCAGTGGAATCGCGTCTCTGAC
GCGCTTTCCCTTACCAATTA

Downstream 100 bases:

>100_bases
GCCCGGTGCCGGCAGGGGCGAATTATCGCCCCTATGGTGACATCAAACAGTATGGGTATAGCGATCGCCGATCTGCGCGG
TGGCATACCAACGCATCACC

Product: DNA-dependent helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 723; Mature: 723

Protein sequence:

>723_residues
MVPMDVSDLLDSLNEKQREAVAAPRGNLLVLAGAGSGKTRVLVHRIAWLLAVENCSPYSIMAVTFTNKAAAEMRHRIEHL
IGTSQGGMWIGTFHGLAHRLLRAHHLDANLPQDFQILDSDDQFRLLKRIVKALNIDEKQWPPRQAMWYINGKKDEGLRPQ
HVETYNNPVEATWLRIYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILNHYRERFTNVLVDEFQDTNSIQYAWIRLLA
GNNSNVMIVGDDDQSIYGWRGAQVENIQRFLKDFPGAQSVLLEQNYRSTSNILKSANTLIANNGGRMGKNLWTEGGEGEP
ISVYCAFNELDESRFVVGRIKAWQENGGALNDCAILYRSNAQSRVLEEALLQTAMPYRIYGGQRFFERQEIKDALAYLRL
IANRNDDAAFERVVNTPTRGIGDRTLDEVRRTARDRQLTMWQATRVLLQDKVLAGRAASALQRFTELLDSLAHETADMPL
HVQTDRVIRDSGLFIMYEQEKGEKGQARIENLEELVTATRQYSYQDEDQDLMPLQAFLSHAALEAGEGQADAYQDAVQLM
TLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGLTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELP
EECVEEVRLRASVSRPANHRRMGTPISENDTGYKLGQRVRHPKFGEGTIVNLEGSGEHSRLQIAFPGEGIKWLVAAYARL
ETV

Sequences:

>Translated_723_residues
MVPMDVSDLLDSLNEKQREAVAAPRGNLLVLAGAGSGKTRVLVHRIAWLLAVENCSPYSIMAVTFTNKAAAEMRHRIEHL
IGTSQGGMWIGTFHGLAHRLLRAHHLDANLPQDFQILDSDDQFRLLKRIVKALNIDEKQWPPRQAMWYINGKKDEGLRPQ
HVETYNNPVEATWLRIYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILNHYRERFTNVLVDEFQDTNSIQYAWIRLLA
GNNSNVMIVGDDDQSIYGWRGAQVENIQRFLKDFPGAQSVLLEQNYRSTSNILKSANTLIANNGGRMGKNLWTEGGEGEP
ISVYCAFNELDESRFVVGRIKAWQENGGALNDCAILYRSNAQSRVLEEALLQTAMPYRIYGGQRFFERQEIKDALAYLRL
IANRNDDAAFERVVNTPTRGIGDRTLDEVRRTARDRQLTMWQATRVLLQDKVLAGRAASALQRFTELLDSLAHETADMPL
HVQTDRVIRDSGLFIMYEQEKGEKGQARIENLEELVTATRQYSYQDEDQDLMPLQAFLSHAALEAGEGQADAYQDAVQLM
TLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGLTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELP
EECVEEVRLRASVSRPANHRRMGTPISENDTGYKLGQRVRHPKFGEGTIVNLEGSGEHSRLQIAFPGEGIKWLVAAYARL
ETV
>Mature_723_residues
MVPMDVSDLLDSLNEKQREAVAAPRGNLLVLAGAGSGKTRVLVHRIAWLLAVENCSPYSIMAVTFTNKAAAEMRHRIEHL
IGTSQGGMWIGTFHGLAHRLLRAHHLDANLPQDFQILDSDDQFRLLKRIVKALNIDEKQWPPRQAMWYINGKKDEGLRPQ
HVETYNNPVEATWLRIYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILNHYRERFTNVLVDEFQDTNSIQYAWIRLLA
GNNSNVMIVGDDDQSIYGWRGAQVENIQRFLKDFPGAQSVLLEQNYRSTSNILKSANTLIANNGGRMGKNLWTEGGEGEP
ISVYCAFNELDESRFVVGRIKAWQENGGALNDCAILYRSNAQSRVLEEALLQTAMPYRIYGGQRFFERQEIKDALAYLRL
IANRNDDAAFERVVNTPTRGIGDRTLDEVRRTARDRQLTMWQATRVLLQDKVLAGRAASALQRFTELLDSLAHETADMPL
HVQTDRVIRDSGLFIMYEQEKGEKGQARIENLEELVTATRQYSYQDEDQDLMPLQAFLSHAALEAGEGQADAYQDAVQLM
TLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGLTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELP
EECVEEVRLRASVSRPANHRRMGTPISENDTGYKLGQRVRHPKFGEGTIVNLEGSGEHSRLQIAFPGEGIKWLVAAYARL
ETV

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=720, Percent_Identity=86.6666666666667, Blast_Score=1328, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=639, Percent_Identity=39.2801251956182, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1787196, Length=340, Percent_Identity=28.5294117647059, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=737, Percent_Identity=27.9511533242877, Blast_Score=190, Evalue=7e-49,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82131; Mature: 82131

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPMDVSDLLDSLNEKQREAVAAPRGNLLVLAGAGSGKTRVLVHRIAWLLAVENCSPYSI
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEE
MAVTFTNKAAAEMRHRIEHLIGTSQGGMWIGTFHGLAHRLLRAHHLDANLPQDFQILDSD
EEEEECCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCEECCCC
DQFRLLKRIVKALNIDEKQWPPRQAMWYINGKKDEGLRPQHVETYNNPVEATWLRIYQAY
CHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHH
QEACDRAGLVDFAELLLRAHELWLNKPHILNHYRERFTNVLVDEFQDTNSIQYAWIRLLA
HHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
GNNSNVMIVGDDDQSIYGWRGAQVENIQRFLKDFPGAQSVLLEQNYRSTSNILKSANTLI
CCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCEE
ANNGGRMGKNLWTEGGEGEPISVYCAFNELDESRFVVGRIKAWQENGGALNDCAILYRSN
ECCCCCCCCHHCCCCCCCCCEEEEEEECCCCCCCEEEHHHHHHHCCCCCCCCEEEEEECC
AQSRVLEEALLQTAMPYRIYGGQRFFERQEIKDALAYLRLIANRNDDAAFERVVNTPTRG
CHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
IGDRTLDEVRRTARDRQLTMWQATRVLLQDKVLAGRAASALQRFTELLDSLAHETADMPL
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
HVQTDRVIRDSGLFIMYEQEKGEKGQARIENLEELVTATRQYSYQDEDQDLMPLQAFLSH
EEECCCEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
AALEAGEGQADAYQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERR
HHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHH
LAYVGLTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRASVSRPANHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHCCCCCCC
RMGTPISENDTGYKLGQRVRHPKFGEGTIVNLEGSGEHSRLQIAFPGEGIKWLVAAYARL
CCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHH
ETV
CCC
>Mature Secondary Structure
MVPMDVSDLLDSLNEKQREAVAAPRGNLLVLAGAGSGKTRVLVHRIAWLLAVENCSPYSI
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEE
MAVTFTNKAAAEMRHRIEHLIGTSQGGMWIGTFHGLAHRLLRAHHLDANLPQDFQILDSD
EEEEECCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCEECCCC
DQFRLLKRIVKALNIDEKQWPPRQAMWYINGKKDEGLRPQHVETYNNPVEATWLRIYQAY
CHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHH
QEACDRAGLVDFAELLLRAHELWLNKPHILNHYRERFTNVLVDEFQDTNSIQYAWIRLLA
HHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
GNNSNVMIVGDDDQSIYGWRGAQVENIQRFLKDFPGAQSVLLEQNYRSTSNILKSANTLI
CCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCEE
ANNGGRMGKNLWTEGGEGEPISVYCAFNELDESRFVVGRIKAWQENGGALNDCAILYRSN
ECCCCCCCCHHCCCCCCCCCEEEEEEECCCCCCCEEEHHHHHHHCCCCCCCCEEEEEECC
AQSRVLEEALLQTAMPYRIYGGQRFFERQEIKDALAYLRLIANRNDDAAFERVVNTPTRG
CHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
IGDRTLDEVRRTARDRQLTMWQATRVLLQDKVLAGRAASALQRFTELLDSLAHETADMPL
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
HVQTDRVIRDSGLFIMYEQEKGEKGQARIENLEELVTATRQYSYQDEDQDLMPLQAFLSH
EEECCCEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
AALEAGEGQADAYQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERR
HHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHH
LAYVGLTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRASVSRPANHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHCCCCCCC
RMGTPISENDTGYKLGQRVRHPKFGEGTIVNLEGSGEHSRLQIAFPGEGIKWLVAAYARL
CCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHH
ETV
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]