Click here to switch to the map view.

The map label for this gene is dapF

Identifier: 157368431

GI number: 157368431

Start: 205915

End: 206739

Strand: Direct

Name: dapF

Synonym: Spro_0182

Alternate gene names: 157368431

Gene position: 205915-206739 (Clockwise)

Preceding gene: 157368430

Following gene: 157368432

Centisome position: 3.78

GC content: 56.36

Gene sequence:

>825_bases
ATGCAGTTCTCCAAAATGCACGGTCTGGGCAACGACTTTATGGTGGTCGATGCCGTTACACAGAATGTCTATTTTTCACC
CGAGCTGATCCGCCGTTTGGCCGATCGGCACCTGGGCGTTGGCTTTGACCAGCTGCTGGTGGTAGAACCGCCTTACGATC
CGGAACTGGATTTCCACTACCGCATTTTTAACGCCGACGGCAGTGAAGTGGCGCAGTGTGGCAACGGCGCTCGCTGTTTT
GCCCGCTTTGTGCGGCTGAAAGGGCTGACCAACAAGCGCGATATTCGCGTGAGCACCCAGACCGGACGTATGGTGCTGAG
TGTCACCGATGACGATTTGGTGTGTGTGAACATGGGCGAGCCGAACTTTGATCCACAGACGGTGCCCTTTCGCGCGACCA
AAGAAGAAAAGACTTACATCATGCGCGCGGCGGAACATACGGTGCTGTGCGGCGTGGTGTCGATGGGCAACCCACATTGT
GTTTTACAGGTGGATGATGTAAAAACCGCCAAGGTGGAATTGCTCGGGCCGGTGCTGGAAGGGCATGAGCGCTTCCCTGA
ACGGGCCAACATTGGTTTTATGCAAATTGTCAGCCGTGAGCACATCAAATTGCGCGTGTATGAGCGCGGTGCCGGCGAAA
CCCAGGCCTGCGGAAGCGGCGCCTGTGCGGCAGTGGCAGTGGGGATCCAACAGGAATTGCTGTCAGAAGAGGTGCATGTA
GAACTGCCGGGCGGCAGCCTGCATATTCGCTGGAAAGGACCGGGCAGCCCGCTGTTTATGACCGGCCCGGCAACCCATGT
ATATGACGGATTTATTCATCTATGA

Upstream 100 bases:

>100_bases
ACTTTCCAGCTCTCAGCAAAAACCGTCGATGAGCGCTCAGTAACACCCTATTTGCGGCGGCACAGGCCACCGCAAACCAA
GACGGATAGCGGAGTATGAT

Downstream 100 bases:

>100_bases
AGAGTGTTGAGGACCAGGCTGTCGCAGGCATTGAGCTTGACGACGACACCGTGATGCAGTTTCTGCTGCAAAACCCGGAC
TTCTTTATCCGCAATGCGCG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQFSKMHGLGNDFMVVDAVTQNVYFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCF
ARFVRLKGLTNKRDIRVSTQTGRMVLSVTDDDLVCVNMGEPNFDPQTVPFRATKEEKTYIMRAAEHTVLCGVVSMGNPHC
VLQVDDVKTAKVELLGPVLEGHERFPERANIGFMQIVSREHIKLRVYERGAGETQACGSGACAAVAVGIQQELLSEEVHV
ELPGGSLHIRWKGPGSPLFMTGPATHVYDGFIHL

Sequences:

>Translated_274_residues
MQFSKMHGLGNDFMVVDAVTQNVYFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCF
ARFVRLKGLTNKRDIRVSTQTGRMVLSVTDDDLVCVNMGEPNFDPQTVPFRATKEEKTYIMRAAEHTVLCGVVSMGNPHC
VLQVDDVKTAKVELLGPVLEGHERFPERANIGFMQIVSREHIKLRVYERGAGETQACGSGACAAVAVGIQQELLSEEVHV
ELPGGSLHIRWKGPGSPLFMTGPATHVYDGFIHL
>Mature_274_residues
MQFSKMHGLGNDFMVVDAVTQNVYFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCF
ARFVRLKGLTNKRDIRVSTQTGRMVLSVTDDDLVCVNMGEPNFDPQTVPFRATKEEKTYIMRAAEHTVLCGVVSMGNPHC
VLQVDDVKTAKVELLGPVLEGHERFPERANIGFMQIVSREHIKLRVYERGAGETQACGSGACAAVAVGIQQELLSEEVHV
ELPGGSLHIRWKGPGSPLFMTGPATHVYDGFIHL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=89.4160583941606, Blast_Score=513, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_SERP5 (A8G852)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476420.1
- ProteinModelPortal:   A8G852
- SMR:   A8G852
- STRING:   A8G852
- GeneID:   5605411
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0182
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   SPRO399741:SPRO_0182-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30339; Mature: 30339

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 73-73 ACT_SITE 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSKMHGLGNDFMVVDAVTQNVYFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHY
CCCHHHCCCCCCEEEEEEEECCEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEE
RIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTQTGRMVLSVTDDDLVCVNMGE
EEECCCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCC
PNFDPQTVPFRATKEEKTYIMRAAEHTVLCGVVSMGNPHCVLQVDDVKTAKVELLGPVLE
CCCCCCCCCEEECCCCCEEEEEECCCEEEEEEEECCCCEEEEEECCCCEEEHEEHHHHHH
GHERFPERANIGFMQIVSREHIKLRVYERGAGETQACGSGACAAVAVGIQQELLSEEVHV
HHHHCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
ELPGGSLHIRWKGPGSPLFMTGPATHVYDGFIHL
EECCCEEEEEEECCCCCEEEECCCHHHCCCEEEC
>Mature Secondary Structure
MQFSKMHGLGNDFMVVDAVTQNVYFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHY
CCCHHHCCCCCCEEEEEEEECCEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEE
RIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTQTGRMVLSVTDDDLVCVNMGE
EEECCCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCC
PNFDPQTVPFRATKEEKTYIMRAAEHTVLCGVVSMGNPHCVLQVDDVKTAKVELLGPVLE
CCCCCCCCCEEECCCCCEEEEEECCCEEEEEEEECCCCEEEEEECCCCEEEHEEHHHHHH
GHERFPERANIGFMQIVSREHIKLRVYERGAGETQACGSGACAAVAVGIQQELLSEEVHV
HHHHCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
ELPGGSLHIRWKGPGSPLFMTGPATHVYDGFIHL
EECCCEEEEEEECCCCCEEEECCCHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA