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The map label for this gene is wecG

Identifier: 157368421

GI number: 157368421

Start: 194339

End: 195079

Strand: Direct

Name: wecG

Synonym: Spro_0172

Alternate gene names: 157368421

Gene position: 194339-195079 (Clockwise)

Preceding gene: 157368420

Following gene: 157368422

Centisome position: 3.57

GC content: 58.03

Gene sequence:

>741_bases
ATGGAAGCAAAAATCTCGGTTCCCAAATATGAGCTGCGCGGTTTCAGCCTGTGGGGATTTCGCGATATGGCGCAATGCAT
GGATTTTCTGTTTGACGGTGGCCGGGTCAAGCGCGGCACGCTGGTTGCCATGAACGCCGAAAAAATCCTGACGGCAGAGC
AGGACACTGAGCTGCATGCCTTGCTGGACGAGGCTGAGTATAAGTATGCCGACGGCATCAGCATGGTGCGCTCAATCCGC
CGTAAATATCCGGGTGCCGACGTGTCTCGGGTAGCGGGAGCCGATCTGTGGGAGGCGCTGATGCAGCGCGCCGGTCGTGA
AGGCACGCCGGTGTTCCTGATTGGCGGCAAGCCGTCGGTGTTGGCAGAAACCGAACAGAAGCTGCGCAGCCAGTGGAATG
TGAATCTGGTCGGTAGCCAGGACGGTTATTTCAAGCCGGAGCAACGTGAACCCCTGTTTGAACGTATCCGTGCCAGCGGG
GCCGCCATAGTAACGGTCGCGATGGGATCGCCAAAACAGGAGATCCTGATGCGTGACTGTCGCAAGGTGCATCCGCAAGC
GTTGTATATGGGCGTAGGCGGGACCTACGACGTATTCACCGGCCATGTTAAGCGTGCGCCAAAGGTCTGGCAGAACCTCG
GCCTGGAATGGCTCTACCGGCTGCTCAGCCAGCCGAGCCGTCTTGGGCGCCAACTGCGGTTGCTGAAGTTCGTGAGTTAT
TATTATCGCGGCAAGATGTAA

Upstream 100 bases:

>100_bases
ACTGCCGGATTGATTAAGGCGCGAGTGCAGCGTTCGCGAACGCTGACACCGCCTGCTTCTTCTGTGCGGGCCGACGGCCT
TTTGTAAGGGTAATAATAAG

Downstream 100 bases:

>100_bases
TCAAGGCGTTGGGGCGGATTTTTCTGCCCCGGCGCAATGAGTTGTTCAATTAACCTGCGTTGTGGCCAATTATCCAGCAC
TCTATAACCTCAGCCGCTAA

Product: putative UDP-N-acetyl-D-mannosaminuronic acid transferase

Products: C55-PP-GlcNAc-ManNAcA; UDP [C]

Alternate protein names: UDP-ManNAcA transferase

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MEAKISVPKYELRGFSLWGFRDMAQCMDFLFDGGRVKRGTLVAMNAEKILTAEQDTELHALLDEAEYKYADGISMVRSIR
RKYPGADVSRVAGADLWEALMQRAGREGTPVFLIGGKPSVLAETEQKLRSQWNVNLVGSQDGYFKPEQREPLFERIRASG
AAIVTVAMGSPKQEILMRDCRKVHPQALYMGVGGTYDVFTGHVKRAPKVWQNLGLEWLYRLLSQPSRLGRQLRLLKFVSY
YYRGKM

Sequences:

>Translated_246_residues
MEAKISVPKYELRGFSLWGFRDMAQCMDFLFDGGRVKRGTLVAMNAEKILTAEQDTELHALLDEAEYKYADGISMVRSIR
RKYPGADVSRVAGADLWEALMQRAGREGTPVFLIGGKPSVLAETEQKLRSQWNVNLVGSQDGYFKPEQREPLFERIRASG
AAIVTVAMGSPKQEILMRDCRKVHPQALYMGVGGTYDVFTGHVKRAPKVWQNLGLEWLYRLLSQPSRLGRQLRLLKFVSY
YYRGKM
>Mature_246_residues
MEAKISVPKYELRGFSLWGFRDMAQCMDFLFDGGRVKRGTLVAMNAEKILTAEQDTELHALLDEAEYKYADGISMVRSIR
RKYPGADVSRVAGADLWEALMQRAGREGTPVFLIGGKPSVLAETEQKLRSQWNVNLVGSQDGYFKPEQREPLFERIRASG
AAIVTVAMGSPKQEILMRDCRKVHPQALYMGVGGTYDVFTGHVKRAPKVWQNLGLEWLYRLLSQPSRLGRQLRLLKFVSY
YYRGKM

Specific function: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II), the second lipid-linked intermediate involved in ECA synthesis

COG id: COG1922

COG function: function code M; Teichoic acid biosynthesis proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 26 family

Homologues:

Organism=Escherichia coli, GI2367289, Length=246, Percent_Identity=71.9512195121951, Blast_Score=367, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WECG_SERP5 (A8G842)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476410.1
- STRING:   A8G842
- GeneID:   5605354
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0172
- eggNOG:   COG1922
- HOGENOM:   HBG685240
- OMA:   ENFIQNN
- ProtClustDB:   PRK03692
- BioCyc:   SPRO399741:SPRO_0172-MONOMER
- HAMAP:   MF_01001
- InterPro:   IPR023085
- InterPro:   IPR004629
- TIGRFAMs:   TIGR00696

Pfam domain/function: PF03808 Glyco_tran_WecB

EC number: 2.4.1.- [C]

Molecular weight: Translated: 27961; Mature: 27961

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKISVPKYELRGFSLWGFRDMAQCMDFLFDGGRVKRGTLVAMNAEKILTAEQDTELHA
CCCCCCCCCCHHCCEEECCHHHHHHHHHHHHCCCCCCCCEEEEECHHHHCCCCCCCHHHH
LLDEAEYKYADGISMVRSIRRKYPGADVSRVAGADLWEALMQRAGREGTPVFLIGGKPSV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHCCCCCCEEEEECCCCCH
LAETEQKLRSQWNVNLVGSQDGYFKPEQREPLFERIRASGAAIVTVAMGSPKQEILMRDC
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
RKVHPQALYMGVGGTYDVFTGHVKRAPKVWQNLGLEWLYRLLSQPSRLGRQLRLLKFVSY
HHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHH
YYRGKM
HHHCCC
>Mature Secondary Structure
MEAKISVPKYELRGFSLWGFRDMAQCMDFLFDGGRVKRGTLVAMNAEKILTAEQDTELHA
CCCCCCCCCCHHCCEEECCHHHHHHHHHHHHCCCCCCCCEEEEECHHHHCCCCCCCHHHH
LLDEAEYKYADGISMVRSIRRKYPGADVSRVAGADLWEALMQRAGREGTPVFLIGGKPSV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHCCCCCCEEEEECCCCCH
LAETEQKLRSQWNVNLVGSQDGYFKPEQREPLFERIRASGAAIVTVAMGSPKQEILMRDC
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
RKVHPQALYMGVGGTYDVFTGHVKRAPKVWQNLGLEWLYRLLSQPSRLGRQLRLLKFVSY
HHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHH
YYRGKM
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosaminuronic-acid; undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate [C]

Specific reaction: UDP-N-acetyl-D-mannosaminuronic-acid + undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate = C55-PP-GlcNAc-ManNAcA + UDP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA