Click here to switch to the map view.

The map label for this gene is rep [H]

Identifier: 157368405

GI number: 157368405

Start: 174559

End: 176583

Strand: Direct

Name: rep [H]

Synonym: Spro_0156

Alternate gene names: 157368405

Gene position: 174559-176583 (Clockwise)

Preceding gene: 157368403

Following gene: 157368408

Centisome position: 3.2

GC content: 54.91

Gene sequence:

>2025_bases
ATGCGATTAAATCCCAGCCAACAACAAGCCGTCGAATTCGTTACCGGGCCCTGTCTGGTGCTGGCCGGTGCCGGTTCCGG
CAAGACGCGCGTCATCACCAACAAAATCGCTCACCTGATCCACAACTGTGGTTATCAGGCGCGGCACATCGCCGCCGTGA
CCTTTACCAATAAGGCGGCGCGTGAGATGAAAGAGCGTGTGGCGCAAACCATGGGGCGCAAAGAAGCACGCGGACTGATG
ATTTCCACTTTCCACACGCTGGGGCTGGAAATCATCAAACGTGAATATGTCGCGCTGGGGATGAAGTCCAACTTCTCACT
TTTCGACGATCAGGATCAGCTGGCGCTGTTGAAAGAACTGACCGAGAAATGGCTGGAGAATGACAAGACGCTGGTAGCGC
AGCTGATTTCCACCATCTCCAACTGGAAGAATGATCTGGTCGATCCCCAGAAGGCGATGGAACTGGCGCGCTCTGAACGC
GACAAGCTGTTCGCGCATTGCTACGGCCTGTATCATGCACATATGCGTGCCTGTAACGTGCTGGATTTTGACGATTTAAT
CCTGCTGCCGACTCTGCTGCTGCAGCGCAATGAAGAAGTGCGCGAACGCTGGCAGAACCGTATTCGTTATCTGTTGGTGG
ATGAATATCAGGACACCAACACCAGTCAGTACGAACTGGTGAAGTTGCTGGTGGGTAATCGCGCACGTTTCACCGTGGTG
GGTGACGACGATCAGTCGATTTACTCCTGGCGTGGTGCGCGTCCACAGAACCTGGTGCTGTTGAAGGAAGACTTCCCGGC
TCTGCAGGTGATTAAGCTGGAGCAGAACTACCGCTCCAGCGAGCGTATCCTGAAGGCGGCGAATATCCTGATCGCCAATA
ACCCGCACGTGTTTGAAAAACGGCTGTTTTCCGAGCTGGGTTACGGCGAAGAGCTGAAGGTGGTGACCGCCAATAATGAG
GATCATGAGGCCGAGCGCGTCGTGGGTGAGCTGATTGCTCATCACTTCGTGAAGAAAACCAACTACGGCGATTATGCGAT
CCTTTATCGTGGTAACCATCAGTCACGGCTGTTTGAAAAAATGCTGATGCAAAACCGCATCCCTTACAAAATTTCCGGTG
GCACCTCGTTTTTCTCGCGGCCGGAAATCAAGGATCTGCTGGCCTACCTGCGTGTTCTGACTAACCAGGATGATGACAGC
GCATTTCTGCGCATCGTTAACACGCCAAAGCGCGAGATTGGCCCGGCAACGCTGCAAAAACTGGGTGAGTGGGCTAATCA
ACGTAACAAAAGCCTGTTCCGCGCCAGTTTCGATCTCGGCCTGAGTCAGCACCTGACCGGTCGTGGGTTAGAGTCGCTGC
AACGCTTCACTCACTGGCTGGACGGGATTGCTCAGTTGGCGGAGCGTGAACCGATAGCCGCGGTGCGCGATCTGATCCGC
GGCGTAGATTACGAAAGTTGGCTGTTTGAAACTTCACCCAGCCCGAAAGCCGCCGAAATGCGCATGAAAAACGTCAATAC
CCTGTTCGGCTGGATGACGGAAATGCTCGAAGGCAGCGAGCTGGACGAGCCAATGACGCTGACCCAGGTGGTAACGCGCT
TTACCCTGCGCGACATGATGGAACGCGGCGAAAGCGACGAGGAACTGGATCAGGTTCAGTTGATGACGCTGCATGCTTCC
AAAGGGCTGGAGTTCCCCTATGTCTTCCTGGTGGGGATGGAGGAGGGGTTACTGCCGCATCAGAGCAGCATTGACGAGGA
TAACGTCGATGAAGAGCGGCGTTTGGCCTATGTAGGGATCACTCGCGCCCAAAAAGAGCTGATTTTCACCCTGTGTCGCG
AACGTCGCCAATATGGCGAACTGGTGCGGCCGGAACCCAGTCGTTTCTTGCTGGAGCTGCCGCAGGATGACCTGGCGTGG
GAAAGTGAACGTAAGGTGGTCAGTCCGCAAGAACGGATGCAAAAAGGGCAGAGCCATCTGGCGAATATTCGCGCCCAGTT
GGCCAAGGCTAAAGGCGGAAGTTAA

Upstream 100 bases:

>100_bases
TTTATCGATACAGAAATTGCGCTGATTATACCCGTTAAATCTCATCGACGCTTTCTGCTACAATCCCCTCCTACGTTATA
AAGTCGAGCAATAAAGCGCC

Downstream 100 bases:

>100_bases
AATTAAGGGCGCTATTGGCGCCCTTCAGACTACTGACAAAGCCCGTTATTAGGGAGAGCGTGCCGAAGGGGTCGTAGCGG
CTTGCCCGCCGGAGCGCCCC

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MRLNPSQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIHNCGYQARHIAAVTFTNKAAREMKERVAQTMGRKEARGLM
ISTFHTLGLEIIKREYVALGMKSNFSLFDDQDQLALLKELTEKWLENDKTLVAQLISTISNWKNDLVDPQKAMELARSER
DKLFAHCYGLYHAHMRACNVLDFDDLILLPTLLLQRNEEVRERWQNRIRYLLVDEYQDTNTSQYELVKLLVGNRARFTVV
GDDDQSIYSWRGARPQNLVLLKEDFPALQVIKLEQNYRSSERILKAANILIANNPHVFEKRLFSELGYGEELKVVTANNE
DHEAERVVGELIAHHFVKKTNYGDYAILYRGNHQSRLFEKMLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNQDDDS
AFLRIVNTPKREIGPATLQKLGEWANQRNKSLFRASFDLGLSQHLTGRGLESLQRFTHWLDGIAQLAEREPIAAVRDLIR
GVDYESWLFETSPSPKAAEMRMKNVNTLFGWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESDEELDQVQLMTLHAS
KGLEFPYVFLVGMEEGLLPHQSSIDEDNVDEERRLAYVGITRAQKELIFTLCRERRQYGELVRPEPSRFLLELPQDDLAW
ESERKVVSPQERMQKGQSHLANIRAQLAKAKGGS

Sequences:

>Translated_674_residues
MRLNPSQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIHNCGYQARHIAAVTFTNKAAREMKERVAQTMGRKEARGLM
ISTFHTLGLEIIKREYVALGMKSNFSLFDDQDQLALLKELTEKWLENDKTLVAQLISTISNWKNDLVDPQKAMELARSER
DKLFAHCYGLYHAHMRACNVLDFDDLILLPTLLLQRNEEVRERWQNRIRYLLVDEYQDTNTSQYELVKLLVGNRARFTVV
GDDDQSIYSWRGARPQNLVLLKEDFPALQVIKLEQNYRSSERILKAANILIANNPHVFEKRLFSELGYGEELKVVTANNE
DHEAERVVGELIAHHFVKKTNYGDYAILYRGNHQSRLFEKMLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNQDDDS
AFLRIVNTPKREIGPATLQKLGEWANQRNKSLFRASFDLGLSQHLTGRGLESLQRFTHWLDGIAQLAEREPIAAVRDLIR
GVDYESWLFETSPSPKAAEMRMKNVNTLFGWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESDEELDQVQLMTLHAS
KGLEFPYVFLVGMEEGLLPHQSSIDEDNVDEERRLAYVGITRAQKELIFTLCRERRQYGELVRPEPSRFLLELPQDDLAW
ESERKVVSPQERMQKGQSHLANIRAQLAKAKGGS
>Mature_674_residues
MRLNPSQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIHNCGYQARHIAAVTFTNKAAREMKERVAQTMGRKEARGLM
ISTFHTLGLEIIKREYVALGMKSNFSLFDDQDQLALLKELTEKWLENDKTLVAQLISTISNWKNDLVDPQKAMELARSER
DKLFAHCYGLYHAHMRACNVLDFDDLILLPTLLLQRNEEVRERWQNRIRYLLVDEYQDTNTSQYELVKLLVGNRARFTVV
GDDDQSIYSWRGARPQNLVLLKEDFPALQVIKLEQNYRSSERILKAANILIANNPHVFEKRLFSELGYGEELKVVTANNE
DHEAERVVGELIAHHFVKKTNYGDYAILYRGNHQSRLFEKMLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNQDDDS
AFLRIVNTPKREIGPATLQKLGEWANQRNKSLFRASFDLGLSQHLTGRGLESLQRFTHWLDGIAQLAEREPIAAVRDLIR
GVDYESWLFETSPSPKAAEMRMKNVNTLFGWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESDEELDQVQLMTLHAS
KGLEFPYVFLVGMEEGLLPHQSSIDEDNVDEERRLAYVGITRAQKELIFTLCRERRQYGELVRPEPSRFLLELPQDDLAW
ESERKVVSPQERMQKGQSHLANIRAQLAKAKGGS

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=672, Percent_Identity=87.2023809523809, Blast_Score=1211, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=656, Percent_Identity=39.4817073170732, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1787196, Length=342, Percent_Identity=27.1929824561404, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=722, Percent_Identity=27.7008310249307, Blast_Score=208, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6324477, Length=687, Percent_Identity=22.707423580786, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77492; Mature: 77492

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLNPSQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIHNCGYQARHIAAVTFTNKAA
CCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHEEEEEEECCHHH
REMKERVAQTMGRKEARGLMISTFHTLGLEIIKREYVALGMKSNFSLFDDQDQLALLKEL
HHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
TEKWLENDKTLVAQLISTISNWKNDLVDPQKAMELARSERDKLFAHCYGLYHAHMRACNV
HHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDFDDLILLPTLLLQRNEEVRERWQNRIRYLLVDEYQDTNTSQYELVKLLVGNRARFTVV
CCCCHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEE
GDDDQSIYSWRGARPQNLVLLKEDFPALQVIKLEQNYRSSERILKAANILIANNPHVFEK
CCCCCHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHCCHHHHHHHHHCEEEECCCHHHHH
RLFSELGYGEELKVVTANNEDHEAERVVGELIAHHFVKKTNYGDYAILYRGNHQSRLFEK
HHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
MLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNQDDDSAFLRIVNTPKREIGPATLQK
HHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCHHHHHH
LGEWANQRNKSLFRASFDLGLSQHLTGRGLESLQRFTHWLDGIAQLAEREPIAAVRDLIR
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GVDYESWLFETSPSPKAAEMRMKNVNTLFGWMTEMLEGSELDEPMTLTQVVTRFTLRDMM
CCCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ERGESDEELDQVQLMTLHASKGLEFPYVFLVGMEEGLLPHQSSIDEDNVDEERRLAYVGI
HCCCCHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHEEEEEH
TRAQKELIFTLCRERRQYGELVRPEPSRFLLELPQDDLAWESERKVVSPQERMQKGQSHL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCHHCCCCCCCCCCCHHHHHHHHHHHH
ANIRAQLAKAKGGS
HHHHHHHHHCCCCC
>Mature Secondary Structure
MRLNPSQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIHNCGYQARHIAAVTFTNKAA
CCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHEEEEEEECCHHH
REMKERVAQTMGRKEARGLMISTFHTLGLEIIKREYVALGMKSNFSLFDDQDQLALLKEL
HHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
TEKWLENDKTLVAQLISTISNWKNDLVDPQKAMELARSERDKLFAHCYGLYHAHMRACNV
HHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDFDDLILLPTLLLQRNEEVRERWQNRIRYLLVDEYQDTNTSQYELVKLLVGNRARFTVV
CCCCHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEE
GDDDQSIYSWRGARPQNLVLLKEDFPALQVIKLEQNYRSSERILKAANILIANNPHVFEK
CCCCCHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHCCHHHHHHHHHCEEEECCCHHHHH
RLFSELGYGEELKVVTANNEDHEAERVVGELIAHHFVKKTNYGDYAILYRGNHQSRLFEK
HHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
MLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNQDDDSAFLRIVNTPKREIGPATLQK
HHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCHHCCHHHHHH
LGEWANQRNKSLFRASFDLGLSQHLTGRGLESLQRFTHWLDGIAQLAEREPIAAVRDLIR
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GVDYESWLFETSPSPKAAEMRMKNVNTLFGWMTEMLEGSELDEPMTLTQVVTRFTLRDMM
CCCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ERGESDEELDQVQLMTLHASKGLEFPYVFLVGMEEGLLPHQSSIDEDNVDEERRLAYVGI
HCCCCHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHEEEEEH
TRAQKELIFTLCRERRQYGELVRPEPSRFLLELPQDDLAWESERKVVSPQERMQKGQSHL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEECCCHHCCCCCCCCCCCHHHHHHHHHHHH
ANIRAQLAKAKGGS
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]