Click here to switch to the map view.

The map label for this gene is dctA [H]

Identifier: 157368402

GI number: 157368402

Start: 171075

End: 172364

Strand: Direct

Name: dctA [H]

Synonym: Spro_0153

Alternate gene names: 157368402

Gene position: 171075-172364 (Clockwise)

Preceding gene: 157368401

Following gene: 157368403

Centisome position: 3.14

GC content: 56.05

Gene sequence:

>1290_bases
ATGAAACTCACTATCTTTAAGAGCCTCTATTTTCAGGTGCTGACAGCGATTACGCTGGGTGTCCTGCTTGGCCATTTCTA
CCCTGACATTGGCGCGCAGATGAAACCTCTGGGCGATGGATTCGTCAAACTGATTAAAATGATCATCGCTCCGGTGATCT
TCTGTACCGTGGTGACCGGTATCGCGGGCATGGAGAGCATGAAGGCCGTCGGTCGCACCGGTGCGATTGCACTGCTCTAT
TTTGAAATTGTCAGCACCATTGCGCTGCTGATTGGTTTGCTGATCGTCAACCTGGTACAGCCGGGTGCCGGGATGAACAT
CGACCCTGGCACGCTGGATGCCAAGGCGGTGGCGGTTTACGCCGAACAAGCCCAGCAGCAGGGCATTGTCCCGTTCCTGC
TGGACATCATCCCCGGCAGCGTGATCGGTGCTTTCGCCAGCGGCAATATTCTGCAGGTGCTGCTGTTTGCCGTGCTGTTT
GGTTTTGCCCTGCATCGCCTGGGCGATAAGGGCCAACTGATCTTTAATGTGATCGAAAGCTTCTCCCGCGTGATCTTCGG
CATCATCAATATGATTATGCGCCTGGCGCCGTTGGGTGCCTTTGGGGCAATGGCGTTCACCATAGGCAAGTATGGCGTGG
GCTCGCTGCTGCAGCTCGGTCAGCTGATCGCCTGCTTCTATCTCACCTGCATTCTGTTCGTGGTGGTGGTGCTGGGCTCG
ATTGCCCGCGCCAACGGCTTTAGCATTTTCAAGTTTGTTCGCTACATCAAAGAAGAGTTGTTGATCGTGCTCGGGACCTC
TTCTTCCGAGTCGGTACTGCCACGCATGCTCGACAAGATGGAAAAGCTGGGCTGCAAAAAATCGGTGGTGGGCCTGGTTA
TTCCGACCGGCTATTCCTTTAACCTGGACGGCACCTCAATCTACCTGACCATGGCCGCGGTGTTTATCGCGCAGGCGACC
AACACCCATATGGATGTTATCCATCAGGTCACGCTGCTGGTGGTGCTGTTGCTGTCCTCTAAAGGGGCTGCAGGGGTGAC
CGGCAGTGGCTTTATCGTGCTGGCCGCCACCATTTCCGCCGTCGGTCATCTGCCGCTGGCCGGTCTGGCGCTGATTCTGG
GCATTGACCGCTTTATGTCCGAAGCCCGTGCGCTGACCAACCTGGTCGGTAACGGTGTGGCCACGGTGGTGGTGGCAAAA
TGGTGTAAACAGCTGGATGAAAAGCAGCTGCATGACACCCTGTCCAACAAGCCTGGCTCAGGAGCCGATAAAACACTGCC
TTCGGCCTGA

Upstream 100 bases:

>100_bases
CCATGTTGTTAGGTGGGTGTTATTTTCCGCGCAAGCGATGAACAGGCGAGATAACTAGACTACCGCCTGGTGCGTGTCTT
CCCCCCCAAAGGATGTTCAT

Downstream 100 bases:

>100_bases
TTTTATCCTCAAATCACCGGTTGCCACGGCAATCGGTGATGTTTTATCCCCCCGCGTAATGATTTCTTGCATTAAAAATA
CGGTTATCCATTATTTTTCA

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKLTIFKSLYFQVLTAITLGVLLGHFYPDIGAQMKPLGDGFVKLIKMIIAPVIFCTVVTGIAGMESMKAVGRTGAIALLY
FEIVSTIALLIGLLIVNLVQPGAGMNIDPGTLDAKAVAVYAEQAQQQGIVPFLLDIIPGSVIGAFASGNILQVLLFAVLF
GFALHRLGDKGQLIFNVIESFSRVIFGIINMIMRLAPLGAFGAMAFTIGKYGVGSLLQLGQLIACFYLTCILFVVVVLGS
IARANGFSIFKFVRYIKEELLIVLGTSSSESVLPRMLDKMEKLGCKKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NTHMDVIHQVTLLVVLLLSSKGAAGVTGSGFIVLAATISAVGHLPLAGLALILGIDRFMSEARALTNLVGNGVATVVVAK
WCKQLDEKQLHDTLSNKPGSGADKTLPSA

Sequences:

>Translated_429_residues
MKLTIFKSLYFQVLTAITLGVLLGHFYPDIGAQMKPLGDGFVKLIKMIIAPVIFCTVVTGIAGMESMKAVGRTGAIALLY
FEIVSTIALLIGLLIVNLVQPGAGMNIDPGTLDAKAVAVYAEQAQQQGIVPFLLDIIPGSVIGAFASGNILQVLLFAVLF
GFALHRLGDKGQLIFNVIESFSRVIFGIINMIMRLAPLGAFGAMAFTIGKYGVGSLLQLGQLIACFYLTCILFVVVVLGS
IARANGFSIFKFVRYIKEELLIVLGTSSSESVLPRMLDKMEKLGCKKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NTHMDVIHQVTLLVVLLLSSKGAAGVTGSGFIVLAATISAVGHLPLAGLALILGIDRFMSEARALTNLVGNGVATVVVAK
WCKQLDEKQLHDTLSNKPGSGADKTLPSA
>Mature_429_residues
MKLTIFKSLYFQVLTAITLGVLLGHFYPDIGAQMKPLGDGFVKLIKMIIAPVIFCTVVTGIAGMESMKAVGRTGAIALLY
FEIVSTIALLIGLLIVNLVQPGAGMNIDPGTLDAKAVAVYAEQAQQQGIVPFLLDIIPGSVIGAFASGNILQVLLFAVLF
GFALHRLGDKGQLIFNVIESFSRVIFGIINMIMRLAPLGAFGAMAFTIGKYGVGSLLQLGQLIACFYLTCILFVVVVLGS
IARANGFSIFKFVRYIKEELLIVLGTSSSESVLPRMLDKMEKLGCKKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQAT
NTHMDVIHQVTLLVVLLLSSKGAAGVTGSGFIVLAATISAVGHLPLAGLALILGIDRFMSEARALTNLVGNGVATVVVAK
WCKQLDEKQLHDTLSNKPGSGADKTLPSA

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI40254478, Length=484, Percent_Identity=26.4462809917355, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI5032093, Length=401, Percent_Identity=27.6807980049875, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI223468564, Length=273, Percent_Identity=30.7692307692308, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI223468566, Length=273, Percent_Identity=30.7692307692308, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI21314632, Length=409, Percent_Identity=27.6283618581907, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI4827012, Length=409, Percent_Identity=26.4058679706601, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI194239697, Length=467, Percent_Identity=23.3404710920771, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI66773030, Length=404, Percent_Identity=25.7425742574257, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI169790839, Length=277, Percent_Identity=28.8808664259928, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI301601644, Length=151, Percent_Identity=30.4635761589404, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI262359914, Length=225, Percent_Identity=28.4444444444444, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1789947, Length=428, Percent_Identity=85.7476635514019, Blast_Score=751, Evalue=0.0,
Organism=Escherichia coli, GI1790514, Length=414, Percent_Identity=39.6135265700483, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI1788024, Length=388, Percent_Identity=22.680412371134, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789473, Length=218, Percent_Identity=25.2293577981651, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17537407, Length=435, Percent_Identity=24.8275862068966, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71983106, Length=469, Percent_Identity=24.5202558635394, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71983099, Length=469, Percent_Identity=24.5202558635394, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71996953, Length=436, Percent_Identity=25.2293577981651, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI193206505, Length=418, Percent_Identity=24.1626794258373, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI193206654, Length=462, Percent_Identity=22.5108225108225, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17541374, Length=402, Percent_Identity=24.6268656716418, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24583025, Length=422, Percent_Identity=21.8009478672986, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI17137668, Length=422, Percent_Identity=21.8009478672986, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24583023, Length=422, Percent_Identity=21.8009478672986, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI281360481, Length=459, Percent_Identity=23.7472766884532, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281360483, Length=459, Percent_Identity=23.7472766884532, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17137666, Length=459, Percent_Identity=23.7472766884532, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45387; Mature: 45387

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTIFKSLYFQVLTAITLGVLLGHFYPDIGAQMKPLGDGFVKLIKMIIAPVIFCTVVTG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAGMESMKAVGRTGAIALLYFEIVSTIALLIGLLIVNLVQPGAGMNIDPGTLDAKAVAVY
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
AEQAQQQGIVPFLLDIIPGSVIGAFASGNILQVLLFAVLFGFALHRLGDKGQLIFNVIES
HHHHHHCCCHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FSRVIFGIINMIMRLAPLGAFGAMAFTIGKYGVGSLLQLGQLIACFYLTCILFVVVVLGS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARANGFSIFKFVRYIKEELLIVLGTSSSESVLPRMLDKMEKLGCKKSVVGLVIPTGYSF
HHHCCCHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCHHHHHEEEEECCEEE
NLDGTSIYLTMAAVFIAQATNTHMDVIHQVTLLVVLLLSSKGAAGVTGSGFIVLAATISA
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
VGHLPLAGLALILGIDRFMSEARALTNLVGNGVATVVVAKWCKQLDEKQLHDTLSNKPGS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GADKTLPSA
CCCCCCCCC
>Mature Secondary Structure
MKLTIFKSLYFQVLTAITLGVLLGHFYPDIGAQMKPLGDGFVKLIKMIIAPVIFCTVVTG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAGMESMKAVGRTGAIALLYFEIVSTIALLIGLLIVNLVQPGAGMNIDPGTLDAKAVAVY
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
AEQAQQQGIVPFLLDIIPGSVIGAFASGNILQVLLFAVLFGFALHRLGDKGQLIFNVIES
HHHHHHCCCHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FSRVIFGIINMIMRLAPLGAFGAMAFTIGKYGVGSLLQLGQLIACFYLTCILFVVVVLGS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARANGFSIFKFVRYIKEELLIVLGTSSSESVLPRMLDKMEKLGCKKSVVGLVIPTGYSF
HHHCCCHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCHHHHHEEEEECCEEE
NLDGTSIYLTMAAVFIAQATNTHMDVIHQVTLLVVLLLSSKGAAGVTGSGFIVLAATISA
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
VGHLPLAGLALILGIDRFMSEARALTNLVGNGVATVVVAKWCKQLDEKQLHDTLSNKPGS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GADKTLPSA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA