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The map label for this gene is intS [H]

Identifier: 157368357

GI number: 157368357

Start: 116464

End: 117633

Strand: Direct

Name: intS [H]

Synonym: Spro_0108

Alternate gene names: 157368357

Gene position: 116464-117633 (Clockwise)

Preceding gene: 157368356

Following gene: 157368358

Centisome position: 2.14

GC content: 49.49

Gene sequence:

>1170_bases
ATGCTCCTAACTGACATACAAATCCGACGTTCCAAACCGCAAGAAAAGCCCTATACCCTCAACGATGGTCAAGGCTTGTC
CCTTCTTATCAATACTGACGGCAGTAAAGGCTGGCGCTTTCGCTATCGCTATGCCGGAAAAGCCAAGTTGATGTCCTTTG
GCACCTACCCCTTGGTAACGCTGGCTGAGGCGCGAGAAAAGCGTGGGGAAGCCCGTAAGCAGGTTGCGAATGGCATTGAC
CCAGTGGCAGATAAAAAGGCGCAAAAGCTCGCGCAGAAACTTTCTTTCGAGAACTCCTTTGAAGCGATTAGCCGTGAATG
GCATACGAACAAGGCTGACCGTTGGACTCTAGCCTACAGGGAAGAGATCATGCGTACCTTTGAGCAGGATGTGTTCCCTC
ATATTGGCCAACGCCCCATTGCCGAAATTAAGCCCTTGGAACTGCTGGAAGTACTGCGGCGTATTGAAAAACGCGGAGCA
TTGGAGAAGACCCGCAAGATTCGCCAGCGCTGTGGAGAGGTATTTCGTTATGCAATTATTACCGGTAGGGCGGAGTATAA
CCCAGCCCCAGATCTCGCCAGCGCGCTAGCCGTTCCCAAACAAAAGCACCATCCGTTCCTTTCGGCCGAGGAACTACCTT
ACTTTGTTAAGGATTTGGAAGGATATACCGGTAGCATCATCACTAAGAATGCAGCGAAGATCGTTATGCTGACCGGCGTG
CGCACGCAAGAAATGCGCTTTGCCACGTGGGAAGAGATCGATTTTGAGCGTGCAATCTGGGAGATCCCCGCAGCACGCAT
GAAAATGCGCCGCCTGCACATTGTGCCGCTCTCTACTCAGGTGGTTGAGCTGTTCAAACAGCTACAACCGATCACCGGTC
ACTATCCTTACATTTTCATTGGACGAAACAACCGCAGAAAGCCTATAAGTAAGGAAAGTGTGAATCAGGTTATAGAGCTG
CTGGGATATAAGGGGCGAGCCACCGGTCACGGTTTCAGGCATACGATGTCCACTATCCTACATGAACAAGGGTTTGATTC
CGCATGGATTGAGATGCAACTTGCGCATGTTGACAAGAATGGTATTCGAGGTACTTATAATCATGCTCAATATTTTGCTC
AGCGACAGTCTATGATGCAATGGTATTCTGACAATATTTTAGTGTTATGA

Upstream 100 bases:

>100_bases
ATTTTTTGTCCGTATAAGTCCGTACATGACTGCCTGAATCTGTGAAAAATGTGTATAGTGATGCGTATAGATTTTTTATA
CACAGAATCCACTTATACAC

Downstream 100 bases:

>100_bases
GGAGGGCTTTTGTGGCTAAAGAAAAGATTGAGGTAAATGATAAGGGTATTCGTAATCGTCTTAAAGCGATTAAGCCTTTT
AATGCAATGTCAGAATATAT

Product: integrase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MLLTDIQIRRSKPQEKPYTLNDGQGLSLLINTDGSKGWRFRYRYAGKAKLMSFGTYPLVTLAEAREKRGEARKQVANGID
PVADKKAQKLAQKLSFENSFEAISREWHTNKADRWTLAYREEIMRTFEQDVFPHIGQRPIAEIKPLELLEVLRRIEKRGA
LEKTRKIRQRCGEVFRYAIITGRAEYNPAPDLASALAVPKQKHHPFLSAEELPYFVKDLEGYTGSIITKNAAKIVMLTGV
RTQEMRFATWEEIDFERAIWEIPAARMKMRRLHIVPLSTQVVELFKQLQPITGHYPYIFIGRNNRRKPISKESVNQVIEL
LGYKGRATGHGFRHTMSTILHEQGFDSAWIEMQLAHVDKNGIRGTYNHAQYFAQRQSMMQWYSDNILVL

Sequences:

>Translated_389_residues
MLLTDIQIRRSKPQEKPYTLNDGQGLSLLINTDGSKGWRFRYRYAGKAKLMSFGTYPLVTLAEAREKRGEARKQVANGID
PVADKKAQKLAQKLSFENSFEAISREWHTNKADRWTLAYREEIMRTFEQDVFPHIGQRPIAEIKPLELLEVLRRIEKRGA
LEKTRKIRQRCGEVFRYAIITGRAEYNPAPDLASALAVPKQKHHPFLSAEELPYFVKDLEGYTGSIITKNAAKIVMLTGV
RTQEMRFATWEEIDFERAIWEIPAARMKMRRLHIVPLSTQVVELFKQLQPITGHYPYIFIGRNNRRKPISKESVNQVIEL
LGYKGRATGHGFRHTMSTILHEQGFDSAWIEMQLAHVDKNGIRGTYNHAQYFAQRQSMMQWYSDNILVL
>Mature_389_residues
MLLTDIQIRRSKPQEKPYTLNDGQGLSLLINTDGSKGWRFRYRYAGKAKLMSFGTYPLVTLAEAREKRGEARKQVANGID
PVADKKAQKLAQKLSFENSFEAISREWHTNKADRWTLAYREEIMRTFEQDVFPHIGQRPIAEIKPLELLEVLRRIEKRGA
LEKTRKIRQRCGEVFRYAIITGRAEYNPAPDLASALAVPKQKHHPFLSAEELPYFVKDLEGYTGSIITKNAAKIVMLTGV
RTQEMRFATWEEIDFERAIWEIPAARMKMRRLHIVPLSTQVVELFKQLQPITGHYPYIFIGRNNRRKPISKESVNQVIEL
LGYKGRATGHGFRHTMSTILHEQGFDSAWIEMQLAHVDKNGIRGTYNHAQYFAQRQSMMQWYSDNILVL

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=384, Percent_Identity=48.1770833333333, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI145693166, Length=387, Percent_Identity=41.343669250646, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1788974, Length=384, Percent_Identity=38.28125, Blast_Score=274, Evalue=8e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45094; Mature: 45094

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTDIQIRRSKPQEKPYTLNDGQGLSLLINTDGSKGWRFRYRYAGKAKLMSFGTYPLVT
CCCCCCCHHCCCCCCCCEEECCCCCEEEEEECCCCCCCEEEEEECCCCCEEECCCCHHHH
LAEAREKRGEARKQVANGIDPVADKKAQKLAQKLSFENSFEAISREWHTNKADRWTLAYR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHH
EEIMRTFEQDVFPHIGQRPIAEIKPLELLEVLRRIEKRGALEKTRKIRQRCGEVFRYAII
HHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TGRAEYNPAPDLASALAVPKQKHHPFLSAEELPYFVKDLEGYTGSIITKNAAKIVMLTGV
CCCCCCCCCHHHHHHHHCCCHHCCCCCCHHHCCHHHHHHCCCCCCEEECCCCEEEEEECC
RTQEMRFATWEEIDFERAIWEIPAARMKMRRLHIVPLSTQVVELFKQLQPITGHYPYIFI
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEECCHHHHHHHHHCCCCCCCCCEEEE
GRNNRRKPISKESVNQVIELLGYKGRATGHGFRHTMSTILHEQGFDSAWIEMQLAHVDKN
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEEEEEECCCC
GIRGTYNHAQYFAQRQSMMQWYSDNILVL
CCCCCHHHHHHHHHHHHHHHHHCCCEEEC
>Mature Secondary Structure
MLLTDIQIRRSKPQEKPYTLNDGQGLSLLINTDGSKGWRFRYRYAGKAKLMSFGTYPLVT
CCCCCCCHHCCCCCCCCEEECCCCCEEEEEECCCCCCCEEEEEECCCCCEEECCCCHHHH
LAEAREKRGEARKQVANGIDPVADKKAQKLAQKLSFENSFEAISREWHTNKADRWTLAYR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHH
EEIMRTFEQDVFPHIGQRPIAEIKPLELLEVLRRIEKRGALEKTRKIRQRCGEVFRYAII
HHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TGRAEYNPAPDLASALAVPKQKHHPFLSAEELPYFVKDLEGYTGSIITKNAAKIVMLTGV
CCCCCCCCCHHHHHHHHCCCHHCCCCCCHHHCCHHHHHHCCCCCCEEECCCCEEEEEECC
RTQEMRFATWEEIDFERAIWEIPAARMKMRRLHIVPLSTQVVELFKQLQPITGHYPYIFI
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEECCHHHHHHHHHCCCCCCCCCEEEE
GRNNRRKPISKESVNQVIELLGYKGRATGHGFRHTMSTILHEQGFDSAWIEMQLAHVDKN
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEEEEEECCCC
GIRGTYNHAQYFAQRQSMMQWYSDNILVL
CCCCCHHHHHHHHHHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]