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The map label for this gene is cynX [H]

Identifier: 157368355

GI number: 157368355

Start: 113483

End: 114682

Strand: Direct

Name: cynX [H]

Synonym: Spro_0106

Alternate gene names: 157368355

Gene position: 113483-114682 (Clockwise)

Preceding gene: 157368353

Following gene: 157368356

Centisome position: 2.08

GC content: 63.08

Gene sequence:

>1200_bases
GTGTTAAATCAATTTGTTGCTCCCCCTTTCTTACGCCGCTGGTTCAACCCCCTGCTGTGGGCACTGGTGCTGATTGGCCT
GAATATGCGGCCGCTGTTGACCTCGATCGGCCCGTTATTGCCGACGTTGCGACAGGCCACCGGGCTGAGCTTTGGCGGTG
CGGCGTTGCTGACCACCTTGCCGGTGCTGATGATGGGGTTGATGGCGCTGGCGGGTGGGGCGATCAACCGCCTGTTCGAC
GAGCGTACCAGCGTAGTGCTGAGCCTGTTGGCCATTGGCCTGGGGGCCGCCTGGCGTGAGCTGGCACCGGGCAGCACGCA
ATTACTGCTGAGCGCGGTGTTGGGTGGACTGGGGATTGGGGTGATCCAGGCGGTGATGCCCGGCATTATCAAACATCACT
TTCTCAAGCAAATGGCGCTGGTGGCCGGGCTGTGGTCGGCGGCTCTGATGGGCGGTGGCGGTCTGGGGGCGGCAATGACC
CCCTGGCTGATGAGTGTCGGCCATGACTGGCACCGTGCATTGGCCTGGTGGGCGGTACCTGCACTGGTGGCGTTAATCGC
CTGGTGGCCGGTCAGCCGTGGCCTGGCACGCCTGACGCCATCCGGAACCTCCTCTGGGCCGAGCCTGTGGTTCAGCCGTC
GTGCCTGGTTGCTCGGTGCCTATTTTGGTTTGATCAATGGCGGCTATACCAGCCTGATCGCCTGGCTGCCGCCGTATTAT
ATGCAGCTCGGCTGGCAGCCGCAGGCCAGCGGATCGCTGTTGGCACTGATGACCTTCGGGCAGGTCGTCGGGGCGCTGCT
GTTGCCGGCGCTGGCGCGATCGCTGGATCGACGGCCGTTGTTATTGCTGGCATTAGTCATGCAGTTGATCGGCTTCATCG
GCCTGATTTATCTGCCGCAGACGTTGCCGTGGCTATGGGTGCTGGTTTCCGGCCTGGGGCTGGGCGGCGCCTTCCCGCTG
TGCCTGGTGTTGGCGCTCGATCATATTCCGCACCCGGCGGCCGCGGGGCGATTGGTGGCCTTTATGCAGGGCATAGGTTT
CCTGCTGGCGGGAGCCACGCCTTATATTTCCGGCCTGTTGCGTGATTACAGCGGCGGCTTTGTGCTGGATTGGCAAATCC
ATGCGCTGCTGGTGGTGGTGCTGATCGCCATCACCTGGTGCTTCCATCCGCACAGCTATCGGCGGGCGTTCGCCGAATAA

Upstream 100 bases:

>100_bases
TTAATTTCCCAATACTAACGGGGAAGCCTAGAGTAAGGCAACGTTGCGGTAGCCACTCTGTGACCCCGCACTCTTCATCC
CGGAAGTTCAGGAATAGATC

Downstream 100 bases:

>100_bases
TATTACTGTCATTGCGGTTCCCTATAGTGACGTTGCTTTTACTTCTGGGTGATAACAACAAAAAGGGAACCAAGACATGC
ACATAACCAAAACCCGCCTG

Product: putative cyanate transporter

Products: Proton [Cytoplasm]; Cyanate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MLNQFVAPPFLRRWFNPLLWALVLIGLNMRPLLTSIGPLLPTLRQATGLSFGGAALLTTLPVLMMGLMALAGGAINRLFD
ERTSVVLSLLAIGLGAAWRELAPGSTQLLLSAVLGGLGIGVIQAVMPGIIKHHFLKQMALVAGLWSAALMGGGGLGAAMT
PWLMSVGHDWHRALAWWAVPALVALIAWWPVSRGLARLTPSGTSSGPSLWFSRRAWLLGAYFGLINGGYTSLIAWLPPYY
MQLGWQPQASGSLLALMTFGQVVGALLLPALARSLDRRPLLLLALVMQLIGFIGLIYLPQTLPWLWVLVSGLGLGGAFPL
CLVLALDHIPHPAAAGRLVAFMQGIGFLLAGATPYISGLLRDYSGGFVLDWQIHALLVVVLIAITWCFHPHSYRRAFAE

Sequences:

>Translated_399_residues
MLNQFVAPPFLRRWFNPLLWALVLIGLNMRPLLTSIGPLLPTLRQATGLSFGGAALLTTLPVLMMGLMALAGGAINRLFD
ERTSVVLSLLAIGLGAAWRELAPGSTQLLLSAVLGGLGIGVIQAVMPGIIKHHFLKQMALVAGLWSAALMGGGGLGAAMT
PWLMSVGHDWHRALAWWAVPALVALIAWWPVSRGLARLTPSGTSSGPSLWFSRRAWLLGAYFGLINGGYTSLIAWLPPYY
MQLGWQPQASGSLLALMTFGQVVGALLLPALARSLDRRPLLLLALVMQLIGFIGLIYLPQTLPWLWVLVSGLGLGGAFPL
CLVLALDHIPHPAAAGRLVAFMQGIGFLLAGATPYISGLLRDYSGGFVLDWQIHALLVVVLIAITWCFHPHSYRRAFAE
>Mature_399_residues
MLNQFVAPPFLRRWFNPLLWALVLIGLNMRPLLTSIGPLLPTLRQATGLSFGGAALLTTLPVLMMGLMALAGGAINRLFD
ERTSVVLSLLAIGLGAAWRELAPGSTQLLLSAVLGGLGIGVIQAVMPGIIKHHFLKQMALVAGLWSAALMGGGGLGAAMT
PWLMSVGHDWHRALAWWAVPALVALIAWWPVSRGLARLTPSGTSSGPSLWFSRRAWLLGAYFGLINGGYTSLIAWLPPYY
MQLGWQPQASGSLLALMTFGQVVGALLLPALARSLDRRPLLLLALVMQLIGFIGLIYLPQTLPWLWVLVSGLGLGGAFPL
CLVLALDHIPHPAAAGRLVAFMQGIGFLLAGATPYISGLLRDYSGGFVLDWQIHALLVVVLIAITWCFHPHSYRRAFAE

Specific function: This protein is part of an active transport system that transports exogenous cyanate into E.coli cells [H]

COG id: COG2807

COG function: function code P; Cyanate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Cyanate porter (TC 2.A.1.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786536, Length=376, Percent_Identity=61.436170212766, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1788092, Length=369, Percent_Identity=30.0813008130081, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004747
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42818; Mature: 42818

Theoretical pI: Translated: 11.02; Mature: 11.02

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure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HHCCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; Cyanate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + Cyanate [Periplasm] = Proton [Cytoplasm] + Cyanate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3049588; 9278503 [H]