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The map label for this gene is tag [H]

Identifier: 157368313

GI number: 157368313

Start: 63625

End: 64188

Strand: Reverse

Name: tag [H]

Synonym: Spro_0064

Alternate gene names: 157368313

Gene position: 64188-63625 (Counterclockwise)

Preceding gene: 157368316

Following gene: 157368312

Centisome position: 1.18

GC content: 56.03

Gene sequence:

>564_bases
ATGATCAACGAACGTTGCGGTTGGGTGACGGCAGATCCGTTATATCTCGAGTATCACGACAAGGAATGGGGCGCACCGAC
GACCGATGCGCGTGAACTGTTCGAAATGCTGTGTCTGGAAGGGCAACAGGCCGGGCTTTCCTGGATCACCGTGCTAAAGA
AACGTGAAAACTACCGTCGTGCCTTCCACAACTTCGATCCACAGCGGGTGGCGGCCATCAGCGAACAAGAGGTGGAAACC
TTGCTGCAGGACAGCGGCATTATCCGCCACCGGGGCAAAATCGAGGCCATCATTGCCAACGCCAGGGCCTACCTGGCGAT
GGAAGCAGCCGGTGAAGACTTCGTCAGCTTTATCTGGACCTTTGTCGACGGCAAGCCACTGCTCAACCGTTGGCAACAAT
TGAGCGAAGTACCGGCGAAAACCGAACGCTCGGACGCCATGTCCAAGGCGCTGAAAAAGCGCGGCTTCAAATTTATCGGA
TCCACCACCTGTTATGCCTTTATGCAGGCCAGTGGGCTGGTGAACGATCATCTGACCGGATGCCTCTGCTACCCAAAGCC
GTAG

Upstream 100 bases:

>100_bases
CCACGTCAAGCCGGTCGTTGATAAAACAATCAGGGGCTTGCCAAGTTCGCAACTTCGGGCGATGCTTACTGTAAATAAAC
ACAGCACAAGGACAGTCAGT

Downstream 100 bases:

>100_bases
CGATCCACCCTTGCGGCCCGGCCGATCTGCCGCGGCTGATGGCCCTGTGGTTGCCCAGCACCATCGCCGCCCATCCGTTC
GTGAAGGAAAGCTACTGGCG

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MINERCGWVTADPLYLEYHDKEWGAPTTDARELFEMLCLEGQQAGLSWITVLKKRENYRRAFHNFDPQRVAAISEQEVET
LLQDSGIIRHRGKIEAIIANARAYLAMEAAGEDFVSFIWTFVDGKPLLNRWQQLSEVPAKTERSDAMSKALKKRGFKFIG
STTCYAFMQASGLVNDHLTGCLCYPKP

Sequences:

>Translated_187_residues
MINERCGWVTADPLYLEYHDKEWGAPTTDARELFEMLCLEGQQAGLSWITVLKKRENYRRAFHNFDPQRVAAISEQEVET
LLQDSGIIRHRGKIEAIIANARAYLAMEAAGEDFVSFIWTFVDGKPLLNRWQQLSEVPAKTERSDAMSKALKKRGFKFIG
STTCYAFMQASGLVNDHLTGCLCYPKP
>Mature_187_residues
MINERCGWVTADPLYLEYHDKEWGAPTTDARELFEMLCLEGQQAGLSWITVLKKRENYRRAFHNFDPQRVAAISEQEVET
LLQDSGIIRHRGKIEAIIANARAYLAMEAAGEDFVSFIWTFVDGKPLLNRWQQLSEVPAKTERSDAMSKALKKRGFKFIG
STTCYAFMQASGLVNDHLTGCLCYPKP

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=182, Percent_Identity=69.2307692307692, Blast_Score=287, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 21298; Mature: 21298

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINERCGWVTADPLYLEYHDKEWGAPTTDARELFEMLCLEGQQAGLSWITVLKKRENYRR
CCCCCCCEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AFHNFDPQRVAAISEQEVETLLQDSGIIRHRGKIEAIIANARAYLAMEAAGEDFVSFIWT
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECHHHHEEHHHCCHHHHHHHHH
FVDGKPLLNRWQQLSEVPAKTERSDAMSKALKKRGFKFIGSTTCYAFMQASGLVNDHLTG
HCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHCHHHHHHHHHHCCCCHHCCCE
CLCYPKP
EEECCCC
>Mature Secondary Structure
MINERCGWVTADPLYLEYHDKEWGAPTTDARELFEMLCLEGQQAGLSWITVLKKRENYRR
CCCCCCCEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AFHNFDPQRVAAISEQEVETLLQDSGIIRHRGKIEAIIANARAYLAMEAAGEDFVSFIWT
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECHHHHEEHHHCCHHHHHHHHH
FVDGKPLLNRWQQLSEVPAKTERSDAMSKALKKRGFKFIGSTTCYAFMQASGLVNDHLTG
HCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHCHHHHHHHHHHCCCCHHCCCE
CLCYPKP
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]