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The map label for this gene is dgoD [H]

Identifier: 157368293

GI number: 157368293

Start: 44376

End: 45524

Strand: Direct

Name: dgoD [H]

Synonym: Spro_0044

Alternate gene names: 157368293

Gene position: 44376-45524 (Clockwise)

Preceding gene: 157368292

Following gene: 157368294

Centisome position: 0.81

GC content: 57.35

Gene sequence:

>1149_bases
ATGAAAATAACCAAAATGACCACCTATCGGCTACCGCCGCGCTGGATGTTCCTGAAGATTGAAACCGATGAAGGCATCGT
CGGTTGGGGTGAACCGGTGATTGAAGGGCGCGCACGCAGTGTGGAGGCGGCGGTGCACGAGCTGTCGGAATATGTGGTCG
GCCAGGATCCGGCGCGGATTAACGATATCTGGCAAACCCTCTACCGCGGCGGTTTCTATCGCGGCGGCCCGATCCTGATG
AGTGCGATCGCCGGTATTGATCAGGCGCTGTGGGATATCAAAGGCAAGGCGCTGGGCGTACCGGTTTATCAGCTATTGGG
CGGATTGGTGCGTGACAAAATCAAGGCGTATAGCTGGGTGGGCGGCGATCGTCCTTCTGACGTGATTGCCGGCATCAACA
AGCTGACGGAAATCGGCTTCGACACCTTTAAGCTGAACGGTTGCGAAGAGATGGGCATTATCGACAGTGCGCGCAAGGTG
GATGCTGCGGTGGCGGTGGTGGCGCAGATCCGCGAAGCCTTTGGTAACCGTATCGAGTTCGGTCTGGATTTCCATGGTCG
CGTCGATGCGCCGATGGCAAAAATCCTGATCAAAGAGCTGGAGCCGTACCGTCCGCTGTTTATTGAAGAGCCGGTGCTGG
CGGAACAGGCAGAGTACTATCCGCGCCTGGCGGCGCAGACGCATATCCCGATTGCCGCCGGTGAACGTATGTATTCGCGT
TTTGATTTCAAACGGGTGCTGGCCGATGGCGGCCTGGCGATTATTCAGCCTGATCTGTCTCATGCCGGTGGCATCACCGA
ATGCTTCAAGATTGCCGCCATGGCGGAGACCTATGATGTGGCGCTGGCTCCGCATTGCCCACTGGGGCCGATTGCGCTGG
CTGCCTGTCTGCATGTGGACTTTGTGGCGCGTAACGCGGTGTTCCAGGAGCAGAGCATGGGCATTCACTACAATCAGGGG
GCCGAACTGCTGGACTACGTAGTGAATAAAGACGATTTCAAAATGACCGACGGCCACTTCTACCCACTGACTAAACCGGG
CCTCGGGGTGGAAATTAACGAAGAGCTGGTGATCGAGCGCAGTAAACAAGCGACGGACTGGCGTAATCCCGTGTGGCGTT
ATCCGGACGGCGCAGTTGCCGAGTGGTAA

Upstream 100 bases:

>100_bases
GTGCCGGGTTGGGGGGCGATCTCTATCGCGCCGGCCAATCGGTGGAACGCACCGCTCAGCAGGCTGCCGCTTTCGTTCGG
GCCTATAAGGAAGCCACTCA

Downstream 100 bases:

>100_bases
TTAGCCGTCATCTTTCAAACCGCAGCGTTATTACCTGCAGATACCGACCCCAGTCACGGATTGACGATTTACAGGGTAAT
AACCGATAGCGTTAAGGCTA

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKITKMTTYRLPPRWMFLKIETDEGIVGWGEPVIEGRARSVEAAVHELSEYVVGQDPARINDIWQTLYRGGFYRGGPILM
SAIAGIDQALWDIKGKALGVPVYQLLGGLVRDKIKAYSWVGGDRPSDVIAGINKLTEIGFDTFKLNGCEEMGIIDSARKV
DAAVAVVAQIREAFGNRIEFGLDFHGRVDAPMAKILIKELEPYRPLFIEEPVLAEQAEYYPRLAAQTHIPIAAGERMYSR
FDFKRVLADGGLAIIQPDLSHAGGITECFKIAAMAETYDVALAPHCPLGPIALAACLHVDFVARNAVFQEQSMGIHYNQG
AELLDYVVNKDDFKMTDGHFYPLTKPGLGVEINEELVIERSKQATDWRNPVWRYPDGAVAEW

Sequences:

>Translated_382_residues
MKITKMTTYRLPPRWMFLKIETDEGIVGWGEPVIEGRARSVEAAVHELSEYVVGQDPARINDIWQTLYRGGFYRGGPILM
SAIAGIDQALWDIKGKALGVPVYQLLGGLVRDKIKAYSWVGGDRPSDVIAGINKLTEIGFDTFKLNGCEEMGIIDSARKV
DAAVAVVAQIREAFGNRIEFGLDFHGRVDAPMAKILIKELEPYRPLFIEEPVLAEQAEYYPRLAAQTHIPIAAGERMYSR
FDFKRVLADGGLAIIQPDLSHAGGITECFKIAAMAETYDVALAPHCPLGPIALAACLHVDFVARNAVFQEQSMGIHYNQG
AELLDYVVNKDDFKMTDGHFYPLTKPGLGVEINEELVIERSKQATDWRNPVWRYPDGAVAEW
>Mature_382_residues
MKITKMTTYRLPPRWMFLKIETDEGIVGWGEPVIEGRARSVEAAVHELSEYVVGQDPARINDIWQTLYRGGFYRGGPILM
SAIAGIDQALWDIKGKALGVPVYQLLGGLVRDKIKAYSWVGGDRPSDVIAGINKLTEIGFDTFKLNGCEEMGIIDSARKV
DAAVAVVAQIREAFGNRIEFGLDFHGRVDAPMAKILIKELEPYRPLFIEEPVLAEQAEYYPRLAAQTHIPIAAGERMYSR
FDFKRVLADGGLAIIQPDLSHAGGITECFKIAAMAETYDVALAPHCPLGPIALAACLHVDFVARNAVFQEQSMGIHYNQG
AELLDYVVNKDDFKMTDGHFYPLTKPGLGVEINEELVIERSKQATDWRNPVWRYPDGAVAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=82.1989528795812, Blast_Score=670, Evalue=0.0,
Organism=Escherichia coli, GI1787864, Length=414, Percent_Identity=30.9178743961353, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI226510960, Length=351, Percent_Identity=29.0598290598291, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 42484; Mature: 42484

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITKMTTYRLPPRWMFLKIETDEGIVGWGEPVIEGRARSVEAAVHELSEYVVGQDPARI
CCCEEEEEEECCCCEEEEEEECCCCCEECCCHHHCCCHHHHHHHHHHHHHHHCCCCCHHH
NDIWQTLYRGGFYRGGPILMSAIAGIDQALWDIKGKALGVPVYQLLGGLVRDKIKAYSWV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHCC
GGDRPSDVIAGINKLTEIGFDTFKLNGCEEMGIIDSARKVDAAVAVVAQIREAFGNRIEF
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
GLDFHGRVDAPMAKILIKELEPYRPLFIEEPVLAEQAEYYPRLAAQTHIPIAAGERMYSR
CCCCCCCCCCHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
FDFKRVLADGGLAIIQPDLSHAGGITECFKIAAMAETYDVALAPHCPLGPIALAACLHVD
HHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHH
FVARNAVFQEQSMGIHYNQGAELLDYVVNKDDFKMTDGHFYPLTKPGLGVEINEELVIER
HHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCEECCCEEEECCCCCCCEEECHHHHEEC
SKQATDWRNPVWRYPDGAVAEW
CCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITKMTTYRLPPRWMFLKIETDEGIVGWGEPVIEGRARSVEAAVHELSEYVVGQDPARI
CCCEEEEEEECCCCEEEEEEECCCCCEECCCHHHCCCHHHHHHHHHHHHHHHCCCCCHHH
NDIWQTLYRGGFYRGGPILMSAIAGIDQALWDIKGKALGVPVYQLLGGLVRDKIKAYSWV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHCC
GGDRPSDVIAGINKLTEIGFDTFKLNGCEEMGIIDSARKVDAAVAVVAQIREAFGNRIEF
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
GLDFHGRVDAPMAKILIKELEPYRPLFIEEPVLAEQAEYYPRLAAQTHIPIAAGERMYSR
CCCCCCCCCCHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
FDFKRVLADGGLAIIQPDLSHAGGITECFKIAAMAETYDVALAPHCPLGPIALAACLHVD
HHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHH
FVARNAVFQEQSMGIHYNQGAELLDYVVNKDDFKMTDGHFYPLTKPGLGVEINEELVIER
HHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCEECCCEEEECCCCCCCEEECHHHHEEC
SKQATDWRNPVWRYPDGAVAEW
CCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA