Click here to switch to the map view.

The map label for this gene is ulaG

Identifier: 157163657

GI number: 157163657

Start: 4443297

End: 4444361

Strand: Reverse

Name: ulaG

Synonym: EcHS_A4436

Alternate gene names: 157163657

Gene position: 4444361-4443297 (Counterclockwise)

Preceding gene: 157163664

Following gene: 157163656

Centisome position: 95.71

GC content: 52.3

Gene sequence:

>1065_bases
ATGAGTAAAGTGAAAAGTATCACCCGAGAATCCTGGATCCTGAGCACTTTCCCGGAGTGGGGTAGCTGGCTGAATGAAGA
AATTGAACAAGAGCAGGTCGCCCCTGGCACATTTGCGATGTGGTGGCTAGGCTGCACCGGGATCTGGTTGAAATCAGAAG
GTGGCACCAACGTTTGCGTTGATTTCTGGTGCGGCACTGGCAAACAAAGTCACGGTAACCCGTTAATGAAACAGGGTCAC
CAGATGCAGCGCATGGCTGGCGTGAAAAAACTGCAGCCAAACCTACGCACCACCCCGTTTGTTCTTGATCCGTTTGCGAT
TCGCCAGATCGACGCGGTACTGGCGACTCACGATCACAACGATCATATCGACGTTAACGTCGCTGCTGCCGTGATGCAGA
ACTGTGCTGATGACGTACCATTCATTGGACCGAAAACCTGCGTTGATTTGTGGATTGGCTGGGGCGTACCGAAAGAGCGT
TGCATCGTGGTCAAACCGGGCGATGTTGTAAAAGTGAAAGACATTGAAATTCATGCGCTTGACGCTTTCGACCGTACTGC
ACTGATCACCCTGCCTGCCGATCAAAAAGCGGCTGGCGTACTGCCGGACGGCATGGACGATCGCGCGGTGAACTATCTGT
TCAAAACGCCTGGCGGCTCCCTGTATCACAGCGGCGACTCCCACTACTCTAACTATTATGCGAAGCACGGTAACGAACAT
CAGATCGATGTGGCGTTAGGATCGTATGGTGAGAACCCGCGCGGTATTACCGACAAAATGACCAGCGCCGATATGCTGCG
TATGGGTGAAGCCCTGAATGCGAAAGTGGTTATCCCGTTCCACCACGACATCTGGTCAAACTTCCAGGCCGATCCGCAAG
AGATCCGCGTGCTGTGGGAGATGAAAAAAGATCGTCTGAAGTATGGCTTCAAGCCGTTTATCTGGCAGGTTGGCGGCAAA
TTTACCTGGCCGCTGGATAAAGACAACTTCGAGTACCACTATCCGCGCGGTTTCGATGATTGCTTCACTATTGAACCGGA
TCTGCCGTTCAAGTCATTCCTGTAA

Upstream 100 bases:

>100_bases
GTCAAAAAATGGTCATTGCCTTCCTGAAAAAATAGTGTCAGGATTAATCAAAACCAATCACATATTGATTCGAATTGAAC
TATCATGAAGGTAAATGGCG

Downstream 100 bases:

>100_bases
TCTGTCGTTTATACCGGATAGCGTGGAGAAGTTCGCGCTATCCGGCGTAGTATTTCCTGGCTATTTCAAATATCATCTAA
AAAAATCAAATTTTATCGGA

Product: putative L-ascorbate 6-phosphate lactonase

Products: NA

Alternate protein names: L-ascorbate utilization protein G

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGH
QMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPGGSLYHSGDSHYSNYYAKHGNEH
QIDVALGSYGENPRGITDKMTSADMLRMGEALNAKVVIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGK
FTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL

Sequences:

>Translated_354_residues
MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGH
QMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPGGSLYHSGDSHYSNYYAKHGNEH
QIDVALGSYGENPRGITDKMTSADMLRMGEALNAKVVIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGK
FTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL
>Mature_353_residues
SKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQ
MQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKERC
IVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPGGSLYHSGDSHYSNYYAKHGNEHQ
IDVALGSYGENPRGITDKMTSADMLRMGEALNAKVVIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKF
TWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL

Specific function: Probably catalyzes the hydrolysis of L-ascorbate-6-P into 3-keto-L-gulonate-6-P. Is essential for L-ascorbate utilization under anaerobic conditions

COG id: COG2220

COG function: function code R; Predicted Zn-dependent hydrolases of the beta-lactamase fold

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ulaG family

Homologues:

Organism=Escherichia coli, GI87082387, Length=354, Percent_Identity=100, Blast_Score=740, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ULAG_ECO24 (A7ZV63)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001465692.1
- ProteinModelPortal:   A7ZV63
- SMR:   A7ZV63
- STRING:   A7ZV63
- EnsemblBacteria:   EBESCT00000021802
- GeneID:   5589543
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_4752
- eggNOG:   COG2220
- GeneTree:   EBGT00050000011692
- HOGENOM:   HBG296934
- OMA:   HWDMWKG
- ProtClustDB:   PRK11709
- BioCyc:   ECOL331111:ECE24377A_4752-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01266
- InterPro:   IPR001279
- SMART:   SM00849

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 40061; Mature: 39930

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCV
CCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCEEEE
DFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHN
EEEECCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHEECCCC
DHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKERCIVVKPGDVVKVKDIEIHAL
CCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEEEEEE
DAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPGGSLYHSGDSHYSNYYAKHGNEH
CCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCHHHHHHHHCCCCE
QIDVALGSYGENPRGITDKMTSADMLRMGEALNAKVVIPFHHDIWSNFQADPQEIRVLWE
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHCCCCCCHHHHHHHHH
MKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL
HHHHHHHCCCCCEEEECCCEEECCCCCCCCCEECCCCCCCCEEECCCCCHHHCC
>Mature Secondary Structure 
SKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCV
CHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCEEEE
DFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHN
EEEECCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHEECCCC
DHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKERCIVVKPGDVVKVKDIEIHAL
CCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEEEEEE
DAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPGGSLYHSGDSHYSNYYAKHGNEH
CCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCHHHHHHHHCCCCE
QIDVALGSYGENPRGITDKMTSADMLRMGEALNAKVVIPFHHDIWSNFQADPQEIRVLWE
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHCCCCCCHHHHHHHHH
MKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL
HHHHHHHCCCCCEEEECCCEEECCCCCCCCCEECCCCCCCCEEECCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA