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The map label for this gene is dcuB

Identifier: 157163589

GI number: 157163589

Start: 4371426

End: 4372766

Strand: Reverse

Name: dcuB

Synonym: EcHS_A4364

Alternate gene names: 157163589

Gene position: 4372766-4371426 (Counterclockwise)

Preceding gene: 157163590

Following gene: 157163588

Centisome position: 94.17

GC content: 52.13

Gene sequence:

>1341_bases
ATGTTATTTACTATCCAACTTATCATAATACTGATATGTCTGTTTTATGGTGCCAGAAAGGGTGGTATCGCGCTGGGTTT
ATTAGGCGGTATCGGTCTGGTCATTCTGGTCTTCGTCTTCCACCTTCAGCCAGGTAAACCACCAGTTGATGTCATGCTGG
TTATCATTGCGGTGGTGGCGGCATCGGCGACCTTGCAAGCTTCGGGCGGTCTTGATGTCATGCTGCAAATTGCCGAGAAG
TTGCTGCGCCGCAACCCGAAATATGTCTCAATTGTCGCGCCGTTTGTGACCTGTACACTGACCATTCTTTGCGGTACGGG
TCATGTGGTTTACACCATTCTGCCGATCATCTACGACGTCGCCATTAAGAACAACATCCGTCCGGAACGTCCGATGGCGG
CAAGTTCTATCGGTGCACAGATGGGGATTATCGCCAGTCCGGTGTCGGTTGCGGTCGTGTCTCTGGTTGCAATGCTGGGT
AATGTCACCTTTGATGGTCGCCATCTTGAGTTCCTCGACCTGCTGGCAATCACCATTCCATCGACGTTAATCGGTATCCT
GGCGATCGGTATCTTCAGCTGGTTCCGCGGTAAAGATCTGGATAAAGACGAAGAGTTCCAGAAATTCATCTCCGTACCGG
AAAACCGTGAGTATGTTTACGGTGATACCGCGACGCTGCTCGATAAAAAACTGCCGAAAAGCAACTGGCTGGCAATGTGG
ATTTTCCTCGGTGCAATCGCTGTAGTCGCCCTTCTTGGTGCTGATTCGGACCTGCGTCCATCCTTCGGCGGCAAACCGCT
GTCGATGGTACTGGTTATTCAGATGTTTATGCTGCTGACCGGGGCGCTGATTATTATCCTGACCAAAACCAATCCCGCGT
CTATCTCAAAAAACGAAGTCTTCCGTTCCGGTATGATCGCCATCGTGGCGGTGTACGGTATCGCATGGATGGCAGAAACC
ATGTTCGGTGCGCATATGTCTGAAATTCAGGGCGTACTGGGTGAAATGGTGAAAGAGTATCCGTGGGCCTATGCCATTGT
TCTGCTGCTGGTTTCCAAGTTTGTAAACTCTCAGGCTGCGGCGCTGGCGGCGATTGTTCCGGTCGCGCTGGCGATCGGCG
TTGATCCGGCATACATCGTGGCTTCAGCACCGGCTTGCTACGGTTATTACATCCTGCCGACTTATCCGAGCGATCTGGCA
GCGATTCAGTTTGACCGTTCCGGCACCACCCACATCGGTCGCTTCGTCATCAACCACAGCTTTATTCTGCCGGGGTTGAT
TGGTGTGAGCGTATCGTGCGTCTTCGGCTGGATCTTCGCCGCGATGTACGGGTTCTTATAA

Upstream 100 bases:

>100_bases
CGGATATTTAGTCAGGCTCTGAAAACAGTTCATACAAAACAGAACGTGACTGTGATCTATTCAGCAAAAATTTAAATAGG
ATTATCGCGAGGGTTCACAC

Downstream 100 bases:

>100_bases
ATGCACTTTGCGTGCCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATACAGAGTTACAGGCTGGAAGCTATG
TCAAACAAACCCTTTATCTA

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEK
LLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREYVYGDTATLLDKKLPKSNWLAMW
IFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMFMLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAET
MFGAHMSEIQGVLGEMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLA
AIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL

Sequences:

>Translated_446_residues
MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEK
LLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREYVYGDTATLLDKKLPKSNWLAMW
IFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMFMLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAET
MFGAHMSEIQGVLGEMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLA
AIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL
>Mature_446_residues
MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEK
LLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREYVYGDTATLLDKKLPKSNWLAMW
IFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMFMLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAET
MFGAHMSEIQGVLGEMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLA
AIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family

Homologues:

Organism=Escherichia coli, GI1790565, Length=446, Percent_Identity=100, Blast_Score=890, Evalue=0.0,
Organism=Escherichia coli, GI1790580, Length=444, Percent_Identity=36.4864864864865, Blast_Score=267, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCUB_ECO57 (P0ABP1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91267
- PIR:   F86107
- RefSeq:   NP_290756.1
- RefSeq:   NP_313132.1
- ProteinModelPortal:   P0ABP1
- EnsemblBacteria:   EBESCT00000028671
- EnsemblBacteria:   EBESCT00000059050
- GeneID:   914219
- GeneID:   960001
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5725
- KEGG:   ecs:ECs5105
- GeneTree:   EBGT00050000010823
- HOGENOM:   HBG297083
- OMA:   MGITASP
- ProtClustDB:   PRK12489
- BioCyc:   ECOL83334:ECS5105-MONOMER
- InterPro:   IPR004668
- PIRSF:   PIRSF004539
- TIGRFAMs:   TIGR00770

Pfam domain/function: PF03605 DcuA_DcuB

EC number: NA

Molecular weight: Translated: 47936; Mature: 47936

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x18337838)-; HASH(0x1940d2a8)-; HASH(0x1928cd10)-; HASH(0x18716dd0)-; HASH(0x1931fe2c)-; HASH(0x193786e4)-; HASH(0x18c6aa30)-; HASH(0x40506b94)-; HASH(0x193785e8)-; HASH(0x170e7d8c)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVA
CEEHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDV
HHHHEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLGNVTFDGRHLEFLDLLAITIP
HHHCCCCCCCCCHHHHHCCHHCHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
STLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREYVYGDTATLLDKKLPKSNWLAMW
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEECCHHHHHHHCCCCCCHHHHH
IFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMFMLLTGALIIILTKTNPASISKNEV
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHH
FRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLGEMVKEYPWAYAIVLLLVSKFVNSQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHH
ALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLAAIQFDRSGTTHIGRFVINHS
HHHHHHHHHHHHCCCCHHHEECCCCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHCC
FILPGLIGVSVSCVFGWIFAAMYGFL
HHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVA
CEEHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDV
HHHHEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLGNVTFDGRHLEFLDLLAITIP
HHHCCCCCCCCCHHHHHCCHHCHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
STLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREYVYGDTATLLDKKLPKSNWLAMW
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEECCHHHHHHHCCCCCCHHHHH
IFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMFMLLTGALIIILTKTNPASISKNEV
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHH
FRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLGEMVKEYPWAYAIVLLLVSKFVNSQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHH
ALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLAAIQFDRSGTTHIGRFVINHS
HHHHHHHHHHHHCCCCHHHEECCCCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHCC
FILPGLIGVSVSCVFGWIFAAMYGFL
HHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796