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The map label for this gene is phnL [H]

Identifier: 157163562

GI number: 157163562

Start: 4341785

End: 4342465

Strand: Reverse

Name: phnL [H]

Synonym: EcHS_A4337

Alternate gene names: 157163562

Gene position: 4342465-4341785 (Counterclockwise)

Preceding gene: 157163563

Following gene: 157163561

Centisome position: 93.52

GC content: 60.21

Gene sequence:

>681_bases
ATGATTAACGTACAAAACGTCAGTAAAACCTTCATCCTGCACCAGCAAAACGGCGTGCGCCTGCCCGTCTTACAACGCGC
CTCGCTCACCGTCAACGCAGGTGAATGCGTGGTGCTCCACGGCCATTCCGGCAGCGGCAAATCAACTCTGCTACGCTCGC
TGTACGCCAACTATCTGCCCGACGAAGGGCAAATCCAGATCAAACACGGTGACGAATGGGTAGACCTGGTCACCGCGCCA
GCGCGCAAAGTGGTGGAGATCCGCAAAACTACCATCGGCTGGGTGAGCCAGTTTCTGCGCGTCATCCCGCGTATCTCAGC
ACTGGAAGTGGTGATGCAGCCGCTGCTCGATACAGGCGTTCCGCGTGAAGCCTGCGCCGCTAAAGCCGCGCGTCTTCTCA
CCCGCCTGAACGTGCCGGAACGCCTGTGGCACCTGGCACCATCGACCTTCTCCGGCGGCGAACAGCAGCGCGTCAACATC
GCCCGCGGCTTTATCGTCGACTACCCCATTCTGCTGCTCGACGAACCTACCGCCTCGCTGGACGCCAAAAACAGCGCCGC
GGTGGTGGAACTGATTCACGAAGCCAAAGCCCGTGGCGCAGCCATCGTCGGCATCTTCCATGACGAAGCTGTACGTAATG
ACGTCGCCGACCGCCTGCACCCAATGGGAGCCTCTTCATGA

Upstream 100 bases:

>100_bases
GATGCGGCGTAAACGCCTTATCCGGCCTACAAATGCGCTCCCCGTAGGTCGGATAAGACGCGTCAGCGTCGCATCCGACA
CCCGAACCACGAGGCGAAAA

Downstream 100 bases:

>100_bases
TTATCAATAACGTTAAGCTGGTGCTGGAAAACGAGGTGGTGCACGGTTCGCTGGAGGTGCAGGATGGCGAAATCCGCGCC
TTTGCCGAAAGCCAGAGCCG

Product: phosphonate C-P lyase system protein PhnL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSGSGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAP
ARKVVEIRKTTIGWVSQFLRVIPRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADRLHPMGASS

Sequences:

>Translated_226_residues
MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSGSGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAP
ARKVVEIRKTTIGWVSQFLRVIPRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADRLHPMGASS
>Mature_226_residues
MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSGSGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAP
ARKVVEIRKTTIGWVSQFLRVIPRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADRLHPMGASS

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates. Probably responsible for energy coupling to the transport system [H]

COG id: COG4778

COG function: function code P; ABC-type phosphonate transport system, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI116734710, Length=223, Percent_Identity=25.5605381165919, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790534, Length=226, Percent_Identity=98.2300884955752, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI87081834, Length=209, Percent_Identity=33.9712918660287, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787029, Length=222, Percent_Identity=38.2882882882883, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788225, Length=220, Percent_Identity=33.6363636363636, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787105, Length=192, Percent_Identity=31.7708333333333, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1787089, Length=193, Percent_Identity=32.6424870466321, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789672, Length=222, Percent_Identity=31.0810810810811, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1786703, Length=194, Percent_Identity=35.5670103092784, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786398, Length=219, Percent_Identity=30.1369863013699, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1789032, Length=221, Percent_Identity=33.0316742081448, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1789962, Length=197, Percent_Identity=28.9340101522843, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789873, Length=208, Percent_Identity=29.3269230769231, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790544, Length=225, Percent_Identity=32.4444444444444, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI1788644, Length=204, Percent_Identity=30.3921568627451, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI48994997, Length=190, Percent_Identity=32.6315789473684, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789891, Length=188, Percent_Identity=31.9148936170213, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787759, Length=216, Percent_Identity=30.5555555555556, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788761, Length=230, Percent_Identity=33.0434782608696, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081709, Length=218, Percent_Identity=31.1926605504587, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1786872, Length=224, Percent_Identity=30.8035714285714, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI87081791, Length=220, Percent_Identity=28.6363636363636, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787500, Length=197, Percent_Identity=26.9035532994924, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI145693107, Length=202, Percent_Identity=29.7029702970297, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI48995001, Length=226, Percent_Identity=26.9911504424779, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789991, Length=217, Percent_Identity=27.6497695852535, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787792, Length=211, Percent_Identity=30.8056872037915, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI48994883, Length=235, Percent_Identity=26.3829787234043, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788506, Length=237, Percent_Identity=29.957805907173, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790467, Length=219, Percent_Identity=27.8538812785388, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788450, Length=216, Percent_Identity=31.0185185185185, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082267, Length=187, Percent_Identity=30.4812834224599, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790190, Length=213, Percent_Identity=28.6384976525822, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787112, Length=180, Percent_Identity=30, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787370, Length=198, Percent_Identity=29.2929292929293, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787712, Length=193, Percent_Identity=29.0155440414508, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786253, Length=157, Percent_Identity=35.6687898089172, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790739, Length=209, Percent_Identity=26.7942583732057, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790525, Length=217, Percent_Identity=27.1889400921659, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788897, Length=194, Percent_Identity=30.4123711340206, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787758, Length=197, Percent_Identity=28.4263959390863, Blast_Score=60, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323217, Length=221, Percent_Identity=28.9592760180996, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322640, Length=170, Percent_Identity=37.0588235294118, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319856, Length=198, Percent_Identity=27.7777777777778, Blast_Score=62, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17136662, Length=192, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24659289, Length=197, Percent_Identity=32.48730964467, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593
- InterPro:   IPR012701 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 24685; Mature: 24685

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSGSGKSTLLRSLYANYLP
CCCCCCCCEEEEEECCCCCCCCEEECCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCC
DEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVIPRISALEVVMQPLLDTGV
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
PREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNIARGFIVDYPILLLDEPTASL
CHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCC
DAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADRLHPMGASS
CCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSGSGKSTLLRSLYANYLP
CCCCCCCCEEEEEECCCCCCCCEEECCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCC
DEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVIPRISALEVVMQPLLDTGV
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
PREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNIARGFIVDYPILLLDEPTASL
CHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCC
DAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADRLHPMGASS
CCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]