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The map label for this gene is sthA [H]

Identifier: 157163439

GI number: 157163439

Start: 4189056

End: 4190456

Strand: Reverse

Name: sthA [H]

Synonym: EcHS_A4196

Alternate gene names: 157163439

Gene position: 4190456-4189056 (Counterclockwise)

Preceding gene: 157163442

Following gene: 157163434

Centisome position: 90.24

GC content: 52.18

Gene sequence:

>1401_bases
ATGCCACATTCCTACGATTACGATGCCATAGTAATAGGTTCCGGCCCCGGCGGCGAAGGCGCTGCAATGGGCCTGGTTAA
GCAAGGTGCGCGCGTCGCAGTTATCGAGCGTTATCAAAATGTTGGCGGCGGTTGCACCCACTGGGGCACCATCCCGTCGA
AAGCTCTCCGTCACGCCGTCAGCCGCATTATAGAATTCAATCAAAACCCACTTTACAGCGACCATTCCCGACTGCTCCGC
TCTTCTTTTGCCGATATCCTTAACCATGCCGATAACGTGATTAATCAACAAACGCGCATGCGTCAGGGATTTTACGAACG
TAATCACTGTGAAATATTGCAGGGGAACGCTCGCTTTGTTGACGAGCATACGTTGGCGCTGGATTGCCCGGACGGCAGCG
TTGAAACACTAACCGCTGAAAAATTTATTATTGCCTGCGGCTCTCGTCCATATCATCCAACAGATGTTGATTTCACCCAT
CCACGCATTTACGACAGCGACTCAATTCTTAGCATGCACCACGAACCGCGCCATGTACTTATCTATGGTGCTGGAGTGAT
CGGCTGTGAATATGCGTCGATCTTCCGCGGTATGGATGTAAAAGTGGATCTGATCAACACCCGCGATCGGCTGCTGGCAT
TTCTCGATCAAGAGATGTCAGATTCTCTCTCCTATCACTTCTGGAACAGTGGCGTAGTGATTCGCCACAACGAAGAGTAC
GAGAAGATCGAAGGCTGTGACGACGGTGTGATCATGCATCTGAAGTCGGGTAAAAAACTGAAAGCTGACTGCCTGCTCTA
TGCCAACGGTCGCACCGGTAATACTGATTCGCTGGCGTTACAGAACATTGGGCTGGAAACTGACAGTCGCGGACAGCTGA
AGGTCAACAGCATGTATCAGACCGCACAGCCGCACGTTTACGCGGTGGGCGACGTGATTGGTTATCCGAGCCTGGCGTCG
GCGGCCTATGACCAGGGGCGCATTGCCGCGCAGGCGCTGGTGAAAGGTGAAGCCACCGCACATCTGATTGAAGATATCCC
TACCGGCATTTACACCATCCCGGAAATCAGCTCTGTGGGCAAAACCGAACAGCAGCTGACCGCGATGAAAGTGCCATATG
AAGTGGGCCGCGCCCAGTTCAAACATCTGGCACGTGCACAAATCGTCGGCATGAACGTGGGCACGCTGAAAATTTTGTTC
CATCGGGAAACAAAAGAGATTCTGGGCATTCACTGCTTTGGCGAGCGCGCTGCCGAAATTATTCATATCGGTCAGGCGAT
TATGGAACAGAAAGGTGGCGGCAACACTATTGAGTACTTCGTCAACACCACCTTTAACTACCCGACGATGGCGGAAGCCT
ATCGGGTAGCTGCGCTAAATGGCTTAAACCGCCTGTTTTAA

Upstream 100 bases:

>100_bases
TCAGGATATAGCCAGATAAATGACGGGGATCAATTGGCTTACCCGCGATAAAATGTTACCATTATGTTGCTTTTATGTAT
AAGAACAGGTAAGCCCTACC

Downstream 100 bases:

>100_bases
CACTTTATCGAAATGGCCATCCATTCTTGCGCGGATGGCCTCTGCCAGCTGCTCATAGCGGCTGCGCAGCGGTGAGCCAG
GACGATAAACCAGGCCAATC

Product: soluble pyridine nucleotide transhydrogenase

Products: NA

Alternate protein names: STH; NAD(P)(+) transhydrogenase [B-specific] [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGGCTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLR
SSFADILNHADNVINQQTRMRQGFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFIIACGSRPYHPTDVDFTH
PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQEMSDSLSYHFWNSGVVIRHNEEY
EKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTDSLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLAS
AAYDQGRIAAQALVKGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMNVGTLKILF
HRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF

Sequences:

>Translated_466_residues
MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGGCTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLR
SSFADILNHADNVINQQTRMRQGFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFIIACGSRPYHPTDVDFTH
PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQEMSDSLSYHFWNSGVVIRHNEEY
EKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTDSLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLAS
AAYDQGRIAAQALVKGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMNVGTLKILF
HRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF
>Mature_465_residues
PHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGGCTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRS
SFADILNHADNVINQQTRMRQGFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFIIACGSRPYHPTDVDFTHP
RIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQEMSDSLSYHFWNSGVVIRHNEEYE
KIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTDSLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASA
AYDQGRIAAQALVKGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMNVGTLKILFH
RETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF

Specific function: Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=474, Percent_Identity=28.4810126582279, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI50301238, Length=462, Percent_Identity=26.4069264069264, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI22035672, Length=491, Percent_Identity=26.8839103869654, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI33519430, Length=400, Percent_Identity=25.75, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI33519428, Length=400, Percent_Identity=25.75, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI33519426, Length=400, Percent_Identity=25.75, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI148277071, Length=400, Percent_Identity=25.75, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI148277065, Length=400, Percent_Identity=25.75, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI291045266, Length=476, Percent_Identity=24.1596638655462, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI291045268, Length=350, Percent_Identity=25.1428571428571, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI65787454, Length=270, Percent_Identity=27.4074074074074, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI226437568, Length=270, Percent_Identity=27.4074074074074, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI21389617, Length=270, Percent_Identity=27.4074074074074, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=99.7854077253219, Blast_Score=971, Evalue=0.0,
Organism=Escherichia coli, GI1786307, Length=467, Percent_Identity=27.6231263383298, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87081717, Length=464, Percent_Identity=27.3706896551724, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789915, Length=431, Percent_Identity=27.6102088167053, Blast_Score=142, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=465, Percent_Identity=29.6774193548387, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17557007, Length=477, Percent_Identity=24.3186582809224, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=367, Percent_Identity=25.8855585831063, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71983419, Length=367, Percent_Identity=25.8855585831063, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=286, Percent_Identity=24.8251748251748, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=484, Percent_Identity=28.099173553719, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6325240, Length=471, Percent_Identity=25.6900212314225, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=24.5689655172414, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=29.5983086680761, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24640549, Length=487, Percent_Identity=29.7741273100616, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=29.7741273100616, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24640551, Length=487, Percent_Identity=29.7741273100616, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI17737741, Length=493, Percent_Identity=26.1663286004057, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327
- InterPro:   IPR022962 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.6.1.1 [H]

Molecular weight: Translated: 51575; Mature: 51444

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGGCTHWGTIPSKALRHAV
CCCCCCCCEEEEECCCCCCCHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCCHHHHHHHHH
SRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQGFYERNHCEILQGNARFV
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEE
DEHTLALDCPDGSVETLTAEKFIIACGSRPYHPTDVDFTHPRIYDSDSILSMHHEPRHVL
ECCEEEEECCCCCCEEEEECEEEEEECCCCCCCCCCCCCCCEEECCCCEEEECCCCCEEE
IYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQEMSDSLSYHFWNSGVVIRHNEEY
EEECCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCH
EKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTDSLALQNIGLETDSRGQLKVNSMYQ
HHHCCCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEECCHHH
TAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALVKGEATAHLIEDIPTGIYTIPEISSVG
CCCCCEEEECCHHCCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCEEECCCCHHCC
KTEQQLTAMKVPYEVGRAQFKHLARAQIVGMNVGTLKILFHRETKEILGIHCFGERAAEI
CCHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCHHHEEEEECCHHHHHH
IHIGQAIMEQKGGGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGGCTHWGTIPSKALRHAV
CCCCCCCEEEEECCCCCCCHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCCHHHHHHHHH
SRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQGFYERNHCEILQGNARFV
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEE
DEHTLALDCPDGSVETLTAEKFIIACGSRPYHPTDVDFTHPRIYDSDSILSMHHEPRHVL
ECCEEEEECCCCCCEEEEECEEEEEECCCCCCCCCCCCCCCEEECCCCEEEECCCCCEEE
IYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQEMSDSLSYHFWNSGVVIRHNEEY
EEECCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCH
EKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTDSLALQNIGLETDSRGQLKVNSMYQ
HHHCCCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCEEECCHHH
TAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALVKGEATAHLIEDIPTGIYTIPEISSVG
CCCCCEEEECCHHCCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCEEECCCCHHCC
KTEQQLTAMKVPYEVGRAQFKHLARAQIVGMNVGTLKILFHRETKEILGIHCFGERAAEI
CCHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCHHHEEEEECCHHHHHH
IHIGQAIMEQKGGGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA