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The map label for this gene is frwD

Identifier: 157163430

GI number: 157163430

Start: 4177050

End: 4177391

Strand: Direct

Name: frwD

Synonym: EcHS_A4187

Alternate gene names: 157163430

Gene position: 4177050-4177391 (Clockwise)

Preceding gene: 157163429

Following gene: 157163435

Centisome position: 89.95

GC content: 56.14

Gene sequence:

>342_bases
ATGGCATACCTGGTGGCAGTAACCGCCTGCGTCAGTGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTT
GTGCCTGTTAGAGAAGTGGGGAGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGACGAGGATA
TCCGTCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTG
CAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCA
AACCCATCTTATTCTGGAGTAG

Upstream 100 bases:

>100_bases
TGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCCGATGTGACAACGATGCGCGAAATGGCAGAACGGGCCGGATTTCA
GGTTACCGTGGGAGGTTAAA

Downstream 100 bases:

>100_bases
TCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGACGATATTCCGACGGCGAGCGTTCTGTGTTTTTACGAAACAGACGG
CAGAAGTAGTTGCTGTCGAC

Product: putative fructose-like phosphotransferase EIIB subunit 3

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 3

Number of amino acids: Translated: 113; Mature: 112

Protein sequence:

>113_residues
MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYV
QCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE

Sequences:

>Translated_113_residues
MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYV
QCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE
>Mature_112_residues
AYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYVQ
CSIYAFLREPQRVMSAVRKVLSAPQQTHLILE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain

Homologues:

Organism=Escherichia coli, GI1790390, Length=113, Percent_Identity=100, Blast_Score=231, Evalue=1e-62,
Organism=Escherichia coli, GI1790387, Length=98, Percent_Identity=43.8775510204082, Blast_Score=86, Evalue=5e-19,
Organism=Escherichia coli, GI87082348, Length=70, Percent_Identity=45.7142857142857, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1788492, Length=65, Percent_Identity=44.6153846153846, Blast_Score=62, Evalue=7e-12,
Organism=Escherichia coli, GI1788730, Length=104, Percent_Identity=31.7307692307692, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTFB3_ECOLI (P32676)

Other databases:

- EMBL:   U00006
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D65202
- RefSeq:   AP_003857.1
- RefSeq:   NP_418388.1
- ProteinModelPortal:   P32676
- SMR:   P32676
- STRING:   P32676
- EnsemblBacteria:   EBESCT00000003911
- EnsemblBacteria:   EBESCT00000016492
- GeneID:   948452
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3925
- KEGG:   eco:b3953
- EchoBASE:   EB1856
- EcoGene:   EG11912
- eggNOG:   COG1445
- GeneTree:   EBGT00070000031719
- HOGENOM:   HBG564028
- OMA:   VENRITA
- ProtClustDB:   PRK10427
- BioCyc:   EcoCyc:EG11912-MONOMER
- Genevestigator:   P32676
- GO:   GO:0005737
- GO:   GO:0016020
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353
- TIGRFAMs:   TIGR00829

Pfam domain/function: PF02302 PTS_IIB

EC number: =2.7.1.69

Molecular weight: Translated: 12637; Mature: 12506

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: ACT_SITE 10-10

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALGTENRLADEDIRRADVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHH
LLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE
HEEECHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHCEECC
>Mature Secondary Structure 
AYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALGTENRLADEDIRRADVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHH
LLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE
HEEECHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398