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The map label for this gene is cytR [H]

Identifier: 157163409

GI number: 157163409

Start: 4151440

End: 4152471

Strand: Reverse

Name: cytR [H]

Synonym: EcHS_A4166

Alternate gene names: 157163409

Gene position: 4152471-4151440 (Counterclockwise)

Preceding gene: 157163410

Following gene: 157163408

Centisome position: 89.42

GC content: 52.03

Gene sequence:

>1032_bases
GTGAGTGTGAAAGCGAAGAAGCAGGAAACTGCCGCGACCATGAAAGACGTTGCCCTCAAGGCAAAAGTCTCTACAGCGAC
CGTCTCCCGAGCATTAATGAATCCCGATAAAGTCTCCCAGGCCACCCGTAATCGGGTTGAAAAAGCGGCCCGGGAAGTGG
GTTATTTACCGCAGCCTATGGGGCGCAACGTCAAGCGTAATGAATCCCGCACCATTCTGGTGATTGTCCCGGATATCTGC
GATCCCTTCTTTAGCGAAATTATTCGCGGTATCGAAGTTACGGCGGCAAATCACGGATATCTGGTGCTGATTGGCGACTG
TGCGCATCAAAATCAGCAGGAAAAAACCTTTATCGATTTGATCATCACCAAGCAAATTGATGGCATGTTGTTGCTGGGTT
CAAGGCTGCCGTTTGATGCCAGCATTGAGGAACAGCGTAATCTGCCGCCGATGGTGATGGCGAACGAATTTGCACCGGAG
CTGGAGCTGCCTACAGTTCATATCGACAATCTGACCGCCGCATTTGATGCAGTAAATTATTTATATGAGCAAGGGCATAA
ACGGATTGGCTGTATAGCCGGTCCCGAAGAGATGCCGCTGTGTCACTACCGCCTGCAAGGCTATGTTCAGGCGCTGCGTC
GCTGCGGCATTATGGTTGATCCGCAATACATCGCCCGTGGCGACTTCACCTTCGAAGCCGGAAGCAAAGCGATGCAGCAG
CTGCTTGATCTTCCACAACCGCCTACTGCTGTCTTCTGCCATAGCGATGTGATGGCGCTCGGCGCACTTTCTCAGGCAAA
ACGCCAGGGGCTGAAAGTCCCGGAAGACCTTTCCATAATCGGTTTTGATAACATCGACCTGACGCAATTTTGTGATCCGC
CGCTGACAACCATCGCGCAGCCGCGTTACGAAATCGGTCGGGAAGCTATGCTGTTATTGCTTGATCAAATGCAGGGGCAA
CACGTTGGCAGTGGCTCTCGTTTAATGGACTGCGAACTTATCATCCGGGGATCAACACGCGCGTTACCTTAA

Upstream 100 bases:

>100_bases
TTCATGAAAATTCTGTAACCGTTTTCACGCGCTATCTGCTAAAAATGTTGCCGATGTGAAGTAAACATGGATGTAGTACG
CCTGACGTGCCAGGCGAGGA

Downstream 100 bases:

>100_bases
AGTAAACCTTTAAGACTGCCGTGACTGGTCAAAGGCCCGCCGCTTAAGTAACATGGCGGGCTGACGAACGAATAAATACA
GCGAAACGATAGTGGCACAA

Product: DNA-binding transcriptional regulator CytR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSVKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPMGRNVKRNESRTILVIVPDIC
DPFFSEIIRGIEVTAANHGYLVLIGDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPE
LELPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQ
LLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQ
HVGSGSRLMDCELIIRGSTRALP

Sequences:

>Translated_343_residues
MSVKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPMGRNVKRNESRTILVIVPDIC
DPFFSEIIRGIEVTAANHGYLVLIGDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPE
LELPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQ
LLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQ
HVGSGSRLMDCELIIRGSTRALP
>Mature_342_residues
SVKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPMGRNVKRNESRTILVIVPDICD
PFFSEIIRGIEVTAANHGYLVLIGDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPEL
ELPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQL
LDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQH
VGSGSRLMDCELIIRGSTRALP

Specific function: This protein negatively controls the transcription initiation of genes such as deoCABD, udp, and cdd encoding catabolizing enzymes and nupC, nupG, and tsx encoding transporting and pore-forming proteins. Binds cytidine and adenosine as effectors [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=341, Percent_Identity=99.7067448680352, Blast_Score=704, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=309, Percent_Identity=37.2168284789644, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=35.5987055016181, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789202, Length=291, Percent_Identity=33.6769759450172, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=35.1791530944625, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789068, Length=311, Percent_Identity=30.5466237942122, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI48994940, Length=312, Percent_Identity=29.4871794871795, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1787580, Length=316, Percent_Identity=27.5316455696203, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1790715, Length=313, Percent_Identity=26.517571884984, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=27.7611940298507, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787906, Length=316, Percent_Identity=26.2658227848101, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1789456, Length=340, Percent_Identity=27.0588235294118, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786268, Length=312, Percent_Identity=23.0769230769231, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790689, Length=315, Percent_Identity=25.0793650793651, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38007; Mature: 37875

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLIGDCAHQNQQEKTFIDL
CCCCCCCCCCEEEEEECCCCCHHHHHHHCCEEEEECCCCEEEEEECCCCCCCCHHHHEEE
IITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELELPTVHIDNLTAAFDAVNY
EEECCCCCEEEECCCCCCCCCCHHHCCCCCEEEECCCCCCCCCCEEEECCHHHHHHHHHH
LYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQ
HHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEECCHHHHCCCCEEHHHHHHHHH
LLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQ
HHCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHCCCCHHHHCC
PRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIRGSTRALP
CHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCC
>Mature Secondary Structure 
SVKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRVEKAAREVGYLPQPM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLIGDCAHQNQQEKTFIDL
CCCCCCCCCCEEEEEECCCCCHHHHHHHCCEEEEECCCCEEEEEECCCCCCCCHHHHEEE
IITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELELPTVHIDNLTAAFDAVNY
EEECCCCCEEEECCCCCCCCCCHHHCCCCCEEEECCCCCCCCCCEEEECCHHHHHHHHHH
LYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVDPQYIARGDFTFEAGSKAMQQ
HHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEECCHHHHCCCCEEHHHHHHHHH
LLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLSIIGFDNIDLTQFCDPPLTTIAQ
HHCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHCCCCHHHHCC
PRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIRGSTRALP
CHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]