Click here to switch to the map view.

The map label for this gene is shiA [H]

Identifier: 157163353

GI number: 157163353

Start: 4100094

End: 4101443

Strand: Direct

Name: shiA [H]

Synonym: EcHS_A4108

Alternate gene names: 157163353

Gene position: 4100094-4101443 (Clockwise)

Preceding gene: 157163352

Following gene: 157163354

Centisome position: 88.3

GC content: 46.44

Gene sequence:

>1350_bases
ATGTCTGAAAAGTTACCCGCACCGCGTGAAGGGCTTTCCGGTAAAGCCATGAGACGTGTCGTTATGGGCAGTTTCGCAGG
CGCGTTAATGGAATGGTATGACTTCTTTATTTTTGGCACGGCAGCAGGACTGGTATTTGCACCGCTGTTTTATCCAGACA
GCGATCCTTTTATAGGTCTTATAGCCGCCTTTGCTACCTTTGGCGTTGGTTTTTTGACCCGTCCTTTAGGTGGTATCGTC
TTTGGCCATTTTGGCGATAAAATTGGGCGAAAAATAACACTTATCTGGACACTGGCTATTGTCGGTTGCTCCACATTTTT
GATTGGTTTTATTCCGACGTATCAGGAAATTGGGATCTGGGCACCGCTCATATTAATGGCGCTGCGTTTAATTCAGGGCT
TTGGTCTTGGTGGCGAGTATGGCGGAGCGGCATTGATGACCATAGAATCAGCGCCCGAATCCCGACGCGGATTTTTAGGT
TCACTCCCGCAAACGGCTGCGTCTGTGGGTATTATGCTGGCAACCGGTATTTTTGCTCTCTGTAACCATTTTCTTACTTC
GGAGCAGTTTCTCTCCTGGGGATGGCGCATTCCGTTTTGGCTTTCTGCGGTGATGTTGATCGTTGGGCTGTTTATCCGCC
TGCATACCGAAGAAACGCTGGATTTTCAAAAGCAAAAAACGACGAATAATAAAGAAAAGTCCGTTCCTCCGTTGATTGAA
TTATTCAAAAAACATCCACGAAATATTTTATTGGCACTGGGTGCGAGGCTGGCGGAAAGTGTCTCCTCTAATATTATTAA
CGCCTTTGGTATTGTCTATATTTCCAGCCAATTAGCATTGTCGCGAGATATTCCCCTGACGGGTATGTTGATTGCCTCGG
CGATCGGTATTTTCAGTTGTCCATTGGTTGGATGGTTATCAGATCGTATAGGTCAAAAAAGTCTGTATTTGTCGGGAGCA
GGATTTTGTGTCCTGTTCGCGTTTCCTTTCTTCTTATTACTGGATAGCAAAAGTACGCTCATCATCTGGTGCAGTATGAT
CCTCGGCTACAACTTAGGTCCGACGATGATGTTTGCCGTGCAGCCAACACTTTTCACCCGCATGTTCGGTACCAAAGTAC
GATATACCGGCCTTTCTTTTGCTTATCAATTTTCCGCGATTCTTGGCGGACTAAGCCCCCTTATCGCCTCAAGTTTGCTG
GCGCTAGGGGGAGGAAAACCGTGGTATGTCGCTCTGTTTTTATTTGCTGTCTCAGTTCTTTCCTTTGTTTGCGTCTGGCT
GATTGAGCCGACCGATGAACAAGAAACCGCTTCTTACCGCTACATCAGGGAACAATCTCATGAAAACTGA

Upstream 100 bases:

>100_bases
AAATTAATGCGCATCGGCTTAAACCGATGCGCATTAATTCAAACTGCCCACGTTTAGTGGTTCGTATCCCAGATTTCTCG
CCTACCAGGAGAGGAAAATT

Downstream 100 bases:

>100_bases
ACGAAAAAACGAGCATTACCTGGCGCTACAACAGGCGTTTGACGCGCCATGGCCTGGGCCTGTAGGAGAGTTAGTGACGC
TGGAAAAAGGCAATATTCAC

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGLVFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIV
FGHFGDKIGRKITLIWTLAIVGCSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEETLDFQKQKTTNNKEKSVPPLIE
LFKKHPRNILLALGARLAESVSSNIINAFGIVYISSQLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGA
GFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLSPLIASSLL
ALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN

Sequences:

>Translated_449_residues
MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGLVFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIV
FGHFGDKIGRKITLIWTLAIVGCSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEETLDFQKQKTTNNKEKSVPPLIE
LFKKHPRNILLALGARLAESVSSNIINAFGIVYISSQLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGA
GFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLSPLIASSLL
ALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN
>Mature_448_residues
SEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGLVFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVF
GHFGDKIGRKITLIWTLAIVGCSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLGS
LPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEETLDFQKQKTTNNKEKSVPPLIEL
FKKHPRNILLALGARLAESVSSNIINAFGIVYISSQLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAG
FCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLSPLIASSLLA
LGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=428, Percent_Identity=41.1214953271028, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1790550, Length=402, Percent_Identity=35.5721393034826, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1789941, Length=435, Percent_Identity=37.2413793103448, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788942, Length=418, Percent_Identity=29.4258373205742, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87082404, Length=397, Percent_Identity=25.6926952141058, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082231, Length=451, Percent_Identity=20.3991130820399, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49166; Mature: 49034

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00024 HEMOPEXIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGLVFAPLFYPDSDPFIGL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
IAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVGCSTFLIGFIPTYQEIGIW
HHHHHHHHHHHHHHHHHHHEEHHHHHHHCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHH
APLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLGSLPQTAASVGIMLATGIFAL
HHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHHHH
CNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEETLDFQKQKTTNNKEKSVPPLIE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHH
LFKKHPRNILLALGARLAESVSSNIINAFGIVYISSQLALSRDIPLTGMLIASAIGIFSC
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
PLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAV
HHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEE
QPTLFTRMFGTKVRYTGLSFAYQFSAILGGLSPLIASSLLALGGGKPWYVALFLFAVSVL
CHHHHHHHHCCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SFVCVWLIEPTDEQETASYRYIREQSHEN
HHHHHHHCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]