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The map label for this gene is yihU [H]

Identifier: 157163349

GI number: 157163349

Start: 4095659

End: 4096537

Strand: Direct

Name: yihU [H]

Synonym: EcHS_A4104

Alternate gene names: 157163349

Gene position: 4095659-4096537 (Clockwise)

Preceding gene: 157163346

Following gene: 157163350

Centisome position: 88.2

GC content: 51.99

Gene sequence:

>879_bases
ATGGCAACCATTGCATTTCTCGGGCTGGGGCAGATGGGTAGCCCAATGGCTAACAATCTATTGCAAAAAGGGCATTCATT
GCAGGTATTTGATGTCAATGCACAGGCCGTCGATGCGCTAGTTACGCAGGGGGCCACTGCGGCACAAACCCCAGCGGAAG
CGGCAGCAGGCGCTGAATTTATCATCACTATGCTTCCCAATGGTGACATTGTTCGACAGGTTCTGTTGGGGGAAAAAGGC
GTCTGTGAGGCTGTTTCATCTGACGCGTTATTGATTGATATGTCGACTATTCATCCCTTACAGACCGATGCATTAATACG
GGAGTTACAAGAAAAAGGCATCAGTATGATGGACGCCCCCGTGGGCAGAACGTCCGTGAATGCGATTGATGGCACATTGC
TGATTTTAGCGGGTGGCACTGATGAGCAGATTGCACGTGCACGCCCGATTCTCATGTGCATGGGTAATGAGCTGGTCGAG
GCTGGCGGCCCAGGAATGGGGATCCGCGTGAAGCTAATTAACAACTATATGAGCATTGCGCTTAATGCGCTGTCCTCCGA
GGCCGCTGTGTTATGTGAGTCACTGGGATTAAATCTTGATGTCGCAATTAAGGTGATGAGTGGTACGGCAGCAGGGAAAG
GACATTTTACGACCACCTGGCCTTGCAAAGTGCTGAAAGGCGATCTTTCACCCGCCTTCATGGTGGATCTGGCGTTGAAA
GATCTGCGTATTGCAGTTGACGTCGCTCGGGAGACGGGGGCACCGCTCAATATGGGGATTGCAGCAGAAAGTTATTACGC
TGCAGCAAGTCAAGACGGCAAAGGGCGGCAGGACTGGAGTGCCCTGCTAAATCAGGTTCGCCAGCAGGCTGGTCTTTAA

Upstream 100 bases:

>100_bases
GTGTCGTGTTTTTGAACATAAATTACAAATATGCTCAAAAACAGACGAAAACGAATTTTCAATTGTCATTATTTTGTCAA
TGTCAGACAGGAGAGTATTC

Downstream 100 bases:

>100_bases
TTTTATCTATTAACAGGAGTTTCTATGTCATCCCACTTCACCCCATTTACGCTGAAAGATATCTCCCGCCCAGGTGGTGG
ATTTGCCATGTTAGCTGTCG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQGATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKG
VCEAVSSDALLIDMSTIHPLQTDALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTTTWPCKVLKGDLSPAFMVDLALK
DLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGRQDWSALLNQVRQQAGL

Sequences:

>Translated_292_residues
MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQGATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKG
VCEAVSSDALLIDMSTIHPLQTDALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTTTWPCKVLKGDLSPAFMVDLALK
DLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGRQDWSALLNQVRQQAGL
>Mature_291_residues
ATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQGATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGV
CEAVSSDALLIDMSTIHPLQTDALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVEA
GGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTTTWPCKVLKGDLSPAFMVDLALKD
LRIAVDVARETGAPLNMGIAAESYYAAASQDGKGRQDWSALLNQVRQQAGL

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=294, Percent_Identity=32.312925170068, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI40556376, Length=284, Percent_Identity=26.056338028169, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1790315, Length=292, Percent_Identity=72.6027397260274, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=38.2142857142857, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1786719, Length=282, Percent_Identity=30.4964539007092, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789092, Length=283, Percent_Identity=29.3286219081272, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17557316, Length=313, Percent_Identity=28.7539936102236, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24655230, Length=295, Percent_Identity=32.2033898305085, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI19922568, Length=295, Percent_Identity=32.2033898305085, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24655240, Length=65, Percent_Identity=49.2307692307692, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28574115, Length=287, Percent_Identity=21.9512195121951, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 30433; Mature: 30301

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQGATAAQTPAEAAAGAEF
CCEEEEEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCHHHHCCCEE
IITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQTDALIRELQEKGISMMDAP
EEEECCCCHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEECCC
VGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVEAGGPGMGIRVKLINNYMSIA
CCCCCCCCCCCEEEEEECCCCHHHHHHCCEEEECCCHHHHCCCCCCEEEEEEHHHHHHHH
LNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTTTWPCKVLKGDLSPAFMVDLALK
HHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCEEECCHHEEECCCCHHHHHHHHHH
DLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGRQDWSALLNQVRQQAGL
HHHHHEEEHHHCCCCCEECEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQGATAAQTPAEAAAGAEF
CEEEEEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCHHHHCCCEE
IITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQTDALIRELQEKGISMMDAP
EEEECCCCHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEECCC
VGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVEAGGPGMGIRVKLINNYMSIA
CCCCCCCCCCCEEEEEECCCCHHHHHHCCEEEECCCHHHHCCCCCCEEEEEEHHHHHHHH
LNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTTTWPCKVLKGDLSPAFMVDLALK
HHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCEEECCHHEEECCCCHHHHHHHHHH
DLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGRQDWSALLNQVRQQAGL
HHHHHEEEHHHCCCCCEECEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8346018; 9278503 [H]