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The map label for this gene is ilvD [H]

Identifier: 157163246

GI number: 157163246

Start: 3981891

End: 3983741

Strand: Direct

Name: ilvD [H]

Synonym: EcHS_A3988

Alternate gene names: 157163246

Gene position: 3981891-3983741 (Clockwise)

Preceding gene: 157163245

Following gene: 157163247

Centisome position: 85.75

GC content: 56.35

Gene sequence:

>1851_bases
ATGCCTAAGTATCGTTCCGCTACCACCACCCATGGCCGTAATATGGCGGGGGCCCGCGCACTGTGGCGCGCCACCGGGAT
GACCGACGCCGATTTCGGTAAGCCGATTATCGCGGTTGTGAACTCGTTCACCCAATTTGTACCGGGTCACGTCCATCTGC
GCGATCTCGGTAAACTGGTCGCCGAACAAATTGAAGCGGCTGGCGGCGTTGCCAAAGAGTTCAACACCATTGCGGTGGAT
GATGGGATTGCCATGGGCCACGGGGGGATGCTTTATTCACTGCCATCTCGCGAACTGATCGCTGATTCCGTTGAGTATAT
GGTCAACGCCCACTGCGCCGATGCCATGGTCTGTATCTCCAACTGCGACAAAATCACCCCTGGGATGTTGATGGCTTCCC
TGCGATTAAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAAGCCGGGAAAACCAAGCTGTCCGATCGGATAATC
AAGCTCGATCTGGTTGATGCGATGATCCAGGGCGCAGACCCGAAAGTCTCTGACTCCCAGAGCGATCAGGTTGAACGTTC
CGCCTGCCCAACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGTTTGT
CGCAGCCAGGCAACGGCTCGCTGCTGGCAACCCACGCGGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTT
GAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATATCGCCAGTAAGGCGGCGTTTGAAAA
CGCCATGACGCTGGATATCGCGATGGGTGGATCGACTAACACCGTACTTCACCTGCTGGCGGCGGCGCAGGAAGCGGAAA
TCGACTTCACCATGAGTGATATCGATAAGCTCTCCCGCAAGGTTCCGCAGCTGTGTAAAGTTGCGCCGAGCACCCAGAAA
TACCATATGGAAGATGTTCATCGTGCTGGTGGTGTTATCGGTATTCTCGGCGAACTGGATCGTGCGGGGTTACTGAACCG
TGATGTGAAAAACGTACTTGGCCTGACGTTGCCGCAAACGCTGGAACAATACGACGTTATGCTGACCCAGGATGACGCGG
TAAAAAATATGTTCCGCGCAGGCCCGGCGGGCATTCGGACTACACAGGCATTCTCGCAGGATTGCCGTTGGGATTCTCTC
GATGACGATCGCGCAAACGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGTGCTCTACGG
TAATTTCGCAGAAAACGGCTGCATCGTTAAAACCGCGGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAG
TGTACGAAAGCCAGGACGACGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTAT
GAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGCTCTACCCAACCAGCTTCCTGAAATCAATGGGGCTCGGTAAAGC
CTGTGCGCTGATCACCGACGGTCGTTTCTCTGGCGGCACCTCTGGCCTTTCTATCGGGCACGTCTCACCGGAAGCGGCAA
GCGGCGGCAGCATTGGCCTGATTGAAGACGGCGATCTTATCGCTATCGACATTCCGAACCGTGGTATTCAGTTACAGGTA
AGCGATGCCGAACTGGCGGCGCGTCGTGAAGCGCAGGAAGCCCGGGGTGACAAAGCCTGGACGCCGAAAAACCGTGAACG
TCAGGTTTCCTTTGCGCTGCGTGCCTACGCCAGCCTGGCGACCAGCGCCGACAAAGGTGCGGTGCGCGATAAATCGAAAC
TGGGGGGTTAA

Upstream 100 bases:

>100_bases
GATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAG
ACACTGGGAGTAAATAAAGC

Downstream 100 bases:

>100_bases
TGATGGCTGACTCGCAACCCCTGTCCGGCACCCCGGAAGGTGCCGAATATTTAAGAGCGGTGCTACGCGCGCCGGTCTAT
GAAGCGGCGCAGGTTACGCC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFTQFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVD
DGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDRII
KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLNAGKRIV
ELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQK
YHMEDVHRAGGVIGILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFSQDCRWDSL
DDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTGPAKVYESQDDAVEAILGGKVVAGDVVVIRY
EGPKGGPGMQEMLYPTSFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQV
SDAELAARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG

Sequences:

>Translated_616_residues
MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFTQFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVD
DGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDRII
KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLNAGKRIV
ELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQK
YHMEDVHRAGGVIGILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFSQDCRWDSL
DDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTGPAKVYESQDDAVEAILGGKVVAGDVVVIRY
EGPKGGPGMQEMLYPTSFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQV
SDAELAARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG
>Mature_615_residues
PKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFTQFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDD
GIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDRIIK
LDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLNAGKRIVE
LTKRYYEQNDESALPRNIASKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKY
HMEDVHRAGGVIGILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFSQDCRWDSLD
DDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTGPAKVYESQDDAVEAILGGKVVAGDVVVIRYE
GPKGGPGMQEMLYPTSFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVS
DAELAARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=616, Percent_Identity=99.512987012987, Blast_Score=1263, Evalue=0.0,
Organism=Escherichia coli, GI1788157, Length=539, Percent_Identity=30.0556586270872, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI2367371, Length=570, Percent_Identity=30.1754385964912, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786464, Length=520, Percent_Identity=30.3846153846154, Blast_Score=173, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322476, Length=615, Percent_Identity=37.3983739837398, Blast_Score=343, Evalue=4e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 65560; Mature: 65429

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFTQFVPGHVHLRDLGKLV
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHHCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDRIIKLDLVDAMIQGADPKVSDSQ
CCCCCCHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHEEEHHHHHHHHCCCCCCCCCCC
SDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLNAGKRIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHH
ELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSD
HHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEHHH
IDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIGILGELDRAGLLNRDVKNVLGLTLPQT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHH
LEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFSQDCRWDSLDDDRANGCIRSLEHAYSKDG
HHHHCEEEECCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
GLAVLYGNFAENGCIVKTAGVDDSILKFTGPAKVYESQDDAVEAILGGKVVAGDVVVIRY
CEEEEECCCCCCCCEEEECCCCCCHHEECCCHHHHCCCHHHHHHHHCCEEEECCEEEEEE
EGPKGGPGMQEMLYPTSFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGSIGL
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCEEE
IEDGDLIAIDIPNRGIQLQVSDAELAARREAQEARGDKAWTPKNRERQVSFALRAYASLA
EECCCEEEEECCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
TSADKGAVRDKSKLGG
CCCCCCCCCCHHHCCC
>Mature Secondary Structure 
PKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFTQFVPGHVHLRDLGKLV
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHHCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDRIIKLDLVDAMIQGADPKVSDSQ
CCCCCCHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHEEEHHHHHHHHCCCCCCCCCCC
SDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLNAGKRIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHH
ELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSD
HHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEHHH
IDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIGILGELDRAGLLNRDVKNVLGLTLPQT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHH
LEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFSQDCRWDSLDDDRANGCIRSLEHAYSKDG
HHHHCEEEECCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
GLAVLYGNFAENGCIVKTAGVDDSILKFTGPAKVYESQDDAVEAILGGKVVAGDVVVIRY
CEEEEECCCCCCCCEEEECCCCCCHHEECCCHHHHCCCHHHHHHHHCCEEEECCEEEEEE
EGPKGGPGMQEMLYPTSFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGSIGL
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCCEEE
IEDGDLIAIDIPNRGIQLQVSDAELAARREAQEARGDKAWTPKNRERQVSFALRAYASLA
EECCCEEEEECCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
TSADKGAVRDKSKLGG
CCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA