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The map label for this gene is dgoD1 [H]

Identifier: 157163171

GI number: 157163171

Start: 3899021

End: 3900169

Strand: Reverse

Name: dgoD1 [H]

Synonym: EcHS_A3904

Alternate gene names: 157163171

Gene position: 3900169-3899021 (Counterclockwise)

Preceding gene: 157163172

Following gene: 157163170

Centisome position: 83.99

GC content: 52.74

Gene sequence:

>1149_bases
ATGAAAATCACCAAAATTACCACGTATCGTTTACCTCCCCGCTGGATGTTCCTGAAAATTGAAACCGATGAAGGCGTGGT
CGGTTGGGGCGAGCCCGTGATCGAAGGCCGCGCCCGTACGGTGGAAGCGGCAGTTCACGAGCTGGGTGACTATTTGATTG
GTCAGGATCCATCGCGCATCAATGACTTATGGCAAGTGATGTATCGCGCCGGATTCTATCGCGGCGGTCCGATCCTGATG
AGCGCCATCGCCGGGATTGACCAGGCGTTATGGGATATCAAAGGTAAAGTGCTGAATGCGCCGGTCTGGCAACTGATGGG
CGGCCTGGTTCGCGACAAAATTAAAGCCTACAGTTGGGTCGGCGGCGATCGTCCGGCGGATGTTATCGGCGGCATTAAAA
CCCTGCGCGAAATCGGCTTCGATACCTTCAAACTGAACGGTTGTGAAGAACTGGGGCTAATTGATAACTCCCGCGCGGTA
GATGCGGCAGTCAACACCGTGGCACAAATTCGTGAAGCTTTTGGCAATCAGATTGAGTTTGGTCTTGATTTCCACGGTCG
CGTCAGCGCGCCGATGGCGAAAGTGCTGATTAAAGAACTGGAGCCGTATCGCCCGCTGTTTATTGAAGAGCCGGTGCTGG
CAGAACAAGCCGAATACTACCCGAAACTGGCGGCACAAACGCATATTCCACTGGCGGCGGGTGAGCGCATGTTCTCACGC
TTCGATTTTAAACGCGTGCTGGAGGCAGGCGGTATTTCGATTCTGCAACCGGATCTCTCCCATGCAGGCGGTATTACTGA
ATGCTACAAAATTGCCGGAATGGCAGAAGCCTATGATGTGACCCTTGCGCCGCACTGTCCGCTCGGACCGATTGCACTGG
CGGCTTGCCTGCATATCGACTTTGTTTCCTATAACGCCGTACTTCAGGAACAAAGTATGGGCATTCATTACAACAAAGGC
GCGGAGTTACTCGACTTTGTGAAAAACAAAGAGGACTTCAGCATGGTTGGCGGCTTCTTTAAACCGCTAACGAAACCGGG
CTTAGGCGTGGAAATCGACGAAGCTAAAGTTATTGAGTTCAGTAAAAATGCCCCGGACTGGCGTAATCCGCTCTGGCGTC
ATGAAGATAACAGCGTAGCAGAGTGGTAA

Upstream 100 bases:

>100_bases
GGGCGGGCTTAGGCAGCGATCTCTATCGCGCCGGGCAATCCGTAGAGCGCACCGCGCAGCAGGCAGCAGCATTTGTTAAG
GCGTATCGAGAGGCAGTGCA

Downstream 100 bases:

>100_bases
TTCCTGCCACGTAAGCCCCTCATCGGGCACTAAAACAGCAATACAAAAATATAACCCTCTGTAAATTACAGGGCATGGTG
AGCGGCTTCGCTATGCCCAA

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD 1 [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILM
SAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWVGGDRPADVIGGIKTLREIGFDTFKLNGCEELGLIDNSRAV
DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR
FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKG
AELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW

Sequences:

>Translated_382_residues
MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILM
SAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWVGGDRPADVIGGIKTLREIGFDTFKLNGCEELGLIDNSRAV
DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR
FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKG
AELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW
>Mature_382_residues
MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILM
SAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWVGGDRPADVIGGIKTLREIGFDTFKLNGCEELGLIDNSRAV
DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR
FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKG
AELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=99.738219895288, Blast_Score=783, Evalue=0.0,
Organism=Escherichia coli, GI1787864, Length=414, Percent_Identity=30.1932367149758, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI226510960, Length=243, Percent_Identity=30.8641975308642, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 42465; Mature: 42465

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRI
CCCEEEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
NDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHCC
GGDRPADVIGGIKTLREIGFDTFKLNGCEELGLIDNSRAVDAAVNTVAQIREAFGNQIEF
CCCCCHHHHHHHHHHHHCCCCEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
GLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR
CCCCCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHID
HHHHHHHHHCCCEEECCCHHHCCCHHHHHHHHCCCHHEEEEECCCCCCHHHHHHHHHHHH
FVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEF
HHHHHHHHHHHCCCEEECCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEE
SKNAPDWRNPLWRHEDNSVAEW
CCCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRI
CCCEEEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
NDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHCC
GGDRPADVIGGIKTLREIGFDTFKLNGCEELGLIDNSRAVDAAVNTVAQIREAFGNQIEF
CCCCCHHHHHHHHHHHHCCCCEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
GLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR
CCCCCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHID
HHHHHHHHHCCCEEECCCHHHCCCHHHHHHHHCCCHHEEEEECCCCCCHHHHHHHHHHHH
FVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEF
HHHHHHHHHHHCCCEEECCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEE
SKNAPDWRNPLWRHEDNSVAEW
CCCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA