BacMap

Definition	Escherichia coli HS, complete genome.
Accession	NC_009800
Length	4,643,538

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The map label for this gene is selB [H]

Identifier: 157163069

GI number: 157163069

Start: 3787320

End: 3789164

Strand: Reverse

Name: selB [H]

Synonym: EcHS_A3795

Alternate gene names: 157163069

Gene position: 3789164-3787320 (Counterclockwise)

Preceding gene: 157163070

Following gene: 157163068

Centisome position: 81.6

GC content: 56.42

Gene sequence:

>1845_bases
ATGATTATTGCGACTGCCGGACACGTTGACCACGGCAAAACAACCTTATTGCAGGCGATTACTGGCGTAAATGCTGACCG
TCTGCCGGAAGAAAAAAAGCGCGGCATGACCATCGATCTCGGCTATGCCTACTGGCCGCAGCCGGATGGTCGCGTGCCTG
GTTTTATCGACGTTCCCGGTCATGAAAAGTTTCTTTCCAACATGCTGGCGGGCGTTGGTGGTATCGATCACGCGCTGTTG
GTGGTGGCGTGCGATGACGGCGTGATGGCACAGACCCGTGAGCATCTGGCGATTTTGCAGCTGACCGGTAACCCGATGCT
GACAGTGGCGCTGACCAAAGCCGATCGCGTGGACGAAGCGCGTGTTGATGAGGTTGAACGCCAGGTAAAGGAGGTTCTGC
GGGAATACGGTTTTGCTGAGGCAAAACTGTTTATCACCGCAGCAACCGAAGGTCGGGGAATGGATGCCCTGCGCGAGCAT
CTGCTTCAGTTGCCGGAACGCGAGCACGCCAGCCAACATAGTTTCCGCCTCGCGATTGACCGCGCATTTACCGTAAAAGG
TGCCGGGCTGGTCGTCACCGGTACGGCGTTAAGCGGGGAAGTGAAGGTAGGCGATTCACTCTGGCTGACTGGTGTAAATA
AACCGATGCGTGTACGTGCGCTGCATGCGCAAAACCAGCCAACAGAAACCGCCAATGCCGGGCAGCGTATCGCGCTTAAC
ATCGCGGGTGATGCGGAAAAAGAGCAGATTAACCGTGGCGACTGGCTGCTTGCCGATGTGCCGCCAGAGCCGTTCACACG
GGTGATTGTCGAGCTTCAAACCCATACACCGCTGACCCAGTGGCAGCCGCTGCATATTCACCACGCCGCCAGCCACGTCA
CGGGACGCGTTTCACTGCTGGAAGATAACCTTGCTGAACTGGTCTTCGACACCCCGTTATGGCTGGCAGATAACGACCGC
CTGGTATTGCGCGATATCTCTGCCCGCAACACGCTGGCCGGAGCGCGCGTCGTGATGCTTAACCCGCCGCGTCGCGGTAA
ACGTAAGCCGGAATATCTGCAATGGCTGGCGTCTCTTGCACGGGCGCAGAGCGATGCCGATGCGTTATCTGTTCATCTGG
AACGCGGCGCGGTTAACCTTGCGGATTTCGCCTGGGCGCGCCAGCTCAACGGCGAAGGGATGCGCGAATTGCTGCAACAG
CCTGGTTATATTCAGGCTGGTTATAGCTTGTTGAATGCGCCGGTTGCCGCCCGCTGGCAGCGGAAAATTCTCGACACATT
AGCGACTTATCATGAGCAACATCGCGATGAACCTGGTCCTGGGCGCGAACGTCTGCGACGTATGGCGTTGCCAATGGAAG
ATGAAGCGCTGGTACTGTTGCTGATTGAAAAGATGCGCGAAAGCGGCGACATCCTCAGTCATCACGGTTGGCTGCATCTG
CCAGATCACAAAGCGGGCTTCAGCGAAGAGCAGCAGGCCATCTGGCAAAAAGCAGAGCCGCTGTTTGGTGACGAGCCGTG
GTGGGTGCGTGACCTGGCAAAAGAGACGGGAACCGACGAGCAGGCAATGCGCCTGACGCTGCGCCAGGCGGCGCAGCAAG
GAATAATTACCGCGATCGTCAAAGATCGTTATTACCGTAACGATCGGATTGTCGAATTTGCCAATATGATCCGCGATCTC
GATCAGGAGTGTGGTTCAACCTGCGCGGCGGATTTCCGCGATCGCTTAGGCGTAGGCCGAAAGCTGGCAATTCAGATTCT
GGAATATTTTGACCGCATTGGCTTTACGCGTCGTCGTGGAAATGATCATTTATTGCGCGACGCATTATTATTTCCGGAAA
AATAA

Upstream 100 bases:

>100_bases
AATTGCCAGTGCCGGTGATTGGTCGTATTTATGACGGACGATTGTGGCTGGATTTACGCTGCCTTGAAGATGAGCAACGG
TTTTTGGAGATGTTGTTGAA

Downstream 100 bases:

>100_bases
GGAAATGATTAATTAATTTTTAAAATAAATTAATACAAATTTCTTATGATTTTAAAAAAAGCACATTGTTTAATGAATAC
AATGTGCTTTTTATTAGATT

Product: selenocysteinyl-tRNA-specific translation factor

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLAILQLTGNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGMDALREH
LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRVRALHAQNQPTETANAGQRIALN
IAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHTPLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDR
LVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGEGMRELLQQ
PGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDILSHHGWLHL
PDHKAGFSEEQQAIWQKAEPLFGDEPWWVRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDL
DQECGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK

Sequences:

>Translated_614_residues
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLAILQLTGNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGMDALREH
LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRVRALHAQNQPTETANAGQRIALN
IAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHTPLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDR
LVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGEGMRELLQQ
PGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDILSHHGWLHL
PDHKAGFSEEQQAIWQKAEPLFGDEPWWVRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDL
DQECGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK
>Mature_614_residues
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLAILQLTGNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGMDALREH
LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRVRALHAQNQPTETANAGQRIALN
IAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHTPLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDR
LVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGEGMRELLQQ
PGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDILSHHGWLHL
PDHKAGFSEEQQAIWQKAEPLFGDEPWWVRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDL
DQECGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=371, Percent_Identity=29.6495956873315, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI54607086, Length=285, Percent_Identity=25.9649122807018, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI5729864, Length=376, Percent_Identity=25.531914893617, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI223555963, Length=376, Percent_Identity=25.531914893617, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI45593140, Length=328, Percent_Identity=25, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI53729339, Length=238, Percent_Identity=29.8319327731092, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI53729337, Length=238, Percent_Identity=29.8319327731092, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI82546879, Length=401, Percent_Identity=24.1895261845387, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI194018520, Length=311, Percent_Identity=24.1157556270096, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI194018522, Length=387, Percent_Identity=22.9974160206718, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI194097354, Length=311, Percent_Identity=24.1157556270096, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI2367247, Length=614, Percent_Identity=99.8371335504886, Blast_Score=1248, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=383, Percent_Identity=30.2872062663185, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1789737, Length=377, Percent_Identity=30.5039787798408, Blast_Score=132, Evalue=9e-32,
Organism=Escherichia coli, GI1789559, Length=251, Percent_Identity=29.4820717131474, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789108, Length=293, Percent_Identity=26.6211604095563, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25141371, Length=276, Percent_Identity=32.6086956521739, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17556456, Length=407, Percent_Identity=27.5184275184275, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17506081, Length=289, Percent_Identity=25.9515570934256, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI115532067, Length=328, Percent_Identity=27.1341463414634, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115532065, Length=328, Percent_Identity=26.219512195122, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17552884, Length=404, Percent_Identity=23.5148514851485, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17569207, Length=404, Percent_Identity=23.5148514851485, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994658, Length=234, Percent_Identity=29.9145299145299, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=303, Percent_Identity=32.013201320132, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320863, Length=253, Percent_Identity=28.4584980237154, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6325337, Length=401, Percent_Identity=22.6932668329177, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319594, Length=401, Percent_Identity=22.6932668329177, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324550, Length=175, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320377, Length=310, Percent_Identity=27.0967741935484, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=281, Percent_Identity=34.5195729537367, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI17864358, Length=281, Percent_Identity=34.5195729537367, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI19921738, Length=289, Percent_Identity=32.8719723183391, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922690, Length=260, Percent_Identity=29.2307692307692, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI45550900, Length=375, Percent_Identity=23.2, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652838, Length=407, Percent_Identity=21.8673218673219, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137572, Length=407, Percent_Identity=21.8673218673219, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137380, Length=350, Percent_Identity=24.5714285714286, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45553807, Length=371, Percent_Identity=23.4501347708895, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45553816, Length=371, Percent_Identity=23.4501347708895, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24651721, Length=371, Percent_Identity=23.4501347708895, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17864154, Length=371, Percent_Identity=23.4501347708895, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28572034, Length=161, Percent_Identity=32.9192546583851, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 68844; Mature: 68844

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVPGFIDVPG
CEEEECCCCCCCHHHHHHHHHCCCHHCCCHHHHCCCEEEECEEECCCCCCCCCCEEECCC
HEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLTGNPMLTVALTKADRVDEA
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCEEEEEECCCCEEEEEEECCCCCCHH
RVDEVERQVKEVLREYGFAEAKLFITAATEGRGMDALREHLLQLPEREHASQHSFRLAID
HHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCHHHCCCCCEEEEEE
RAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRVRALHAQNQPTETANAGQRIALN
EEEEEECCCEEEEECEECCCEEECCCEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEEE
IAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHTPLTQWQPLHIHHAASHVTGRVSLL
ECCCCCHHHCCCCCEEEECCCCCHHHHHHEEEECCCCCCCCCCEEEEHHHHHHHHHHHHH
EDNLAELVFDTPLWLADNDRLVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLA
HHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
RAQSDADALSVHLERGAVNLADFAWARQLNGEGMRELLQQPGYIQAGYSLLNAPVAARWQ
HCCCCCHHHEEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHH
RKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDILSHHGWLHL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCEEEC
PDHKAGFSEEQQAIWQKAEPLFGDEPWWVRDLAKETGTDEQAMRLTLRQAAQQGIITAIV
CCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHH
KDRYYRNDRIVEFANMIRDLDQECGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG
HHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCC
NDHLLRDALLFPEK
CCHHHHHHHCCCCC
>Mature Secondary Structure
MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAYWPQPDGRVPGFIDVPG
CEEEECCCCCCCHHHHHHHHHCCCHHCCCHHHHCCCEEEECEEECCCCCCCCCCEEECCC
HEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLTGNPMLTVALTKADRVDEA
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHCCEEEEEECCCCEEEEEEECCCCCCHH
RVDEVERQVKEVLREYGFAEAKLFITAATEGRGMDALREHLLQLPEREHASQHSFRLAID
HHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCHHHCCCCCEEEEEE
RAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRVRALHAQNQPTETANAGQRIALN
EEEEEECCCEEEEECEECCCEEECCCEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEEE
IAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHTPLTQWQPLHIHHAASHVTGRVSLL
ECCCCCHHHCCCCCEEEECCCCCHHHHHHEEEECCCCCCCCCCEEEEHHHHHHHHHHHHH
EDNLAELVFDTPLWLADNDRLVLRDISARNTLAGARVVMLNPPRRGKRKPEYLQWLASLA
HHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
RAQSDADALSVHLERGAVNLADFAWARQLNGEGMRELLQQPGYIQAGYSLLNAPVAARWQ
HCCCCCHHHEEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHH
RKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDILSHHGWLHL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCEEEC
PDHKAGFSEEQQAIWQKAEPLFGDEPWWVRDLAKETGTDEQAMRLTLRQAAQQGIITAIV
CCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHH
KDRYYRNDRIVEFANMIRDLDQECGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG
HHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCC
NDHLLRDALLFPEK
CCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2531290; 8041620; 9278503; 2140572 [H]