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The map label for this gene is bisC [H]

Identifier: 157163026

GI number: 157163026

Start: 3743364

End: 3745697

Strand: Reverse

Name: bisC [H]

Synonym: EcHS_A3752

Alternate gene names: 157163026

Gene position: 3745697-3743364 (Counterclockwise)

Preceding gene: 157163029

Following gene: 157163023

Centisome position: 80.66

GC content: 54.84

Gene sequence:

>2334_bases
TTGGCCAACTCATCCTCACGATATTCCGTTCTGACTGCCGCCCACTGGGGGCCCATGCTGGTTGAAACCGACGGCGAAAC
CGTGTTTAGCTCGCGTGGCGCGTTAGCCACAGGAATGGAAAACTCCTTGCAGAGCGCGGTTCGCGACCAGGTTCACAGCA
ATACGCGGGTACGATTTCCAATGGTGCGCAAAGGCTTTCTCGCGTCACCGGAAAACCCGCAAGGCATTCGTGGGCAGGAT
GAATTTGTTCGCGTGAGTTGGGATGAGGCGCTGGATCTTATTCACCAACAACATAAACGCATTCGTGAGGCTTATGGTCC
GGCATCGATTTTTGCTGGTTCCTACGGCTGGCGTTCAAACGGCGTGCTGCATAAGGCCTCGACATTATTACAACGCTATA
TGGCGCTGGCAGGCGGTTATACCGGGCATCTGGGGGATTATTCGACCGGCGCGGCACAGGCGATCATGCCGTATGTCGTG
GGTGGTAGCGAAGTTTATCAACAGCAGACCAGTTGGCCGCTGGTGCTGGAACATAGCGATGTCGTGGTGCTGTGGAGTGC
TAATCCACTCAATACGCTGAAAATTGCGTGGAATGCATCCGATGAGCAGGGGCTTTCTTACTTTTCTGCACTGCGTGACA
GTGGGAAAAAGCTGATCTGCATTGATCCAATGCGATCGGAAACCGTCGATTTCTTTGGCGATAAAATGGAGTGGGTGGCA
CCGCACATGGGCACCGATGTTGCGCTGATGCTGGGGATCGCCCATACGCTGGTGGAAAATGGTTGGCACGACGAAGCGTT
TCTGGCGCGTTGCACCACAGGTTATGCCGTCTTCGCCTCTTATTTGCTGGGCGAGAGTGACGGAATAGCGAAAACCGCCG
AATGGGCAGCAGAGATTTGTGGTGTTGGCGCAGCGAAAATCCGCGAGCTGGCGGCTATTTTCCACCAAAATACCACCATG
CTGATGGCAGGCTGGGGAATGCAGCGCCAACAGTTTGGTGAGCAAAAACACTGGATGATCGTCACGCTGGCAGCAATGTT
GGGGCAAATCGGCACACCCGGCGGCGGTTTTGGTCTTTCTTACCATTTTGCCAATGGTGGTAACCCCACGCGGCGTTCTG
CGGTGCTCTCTTCCATGCAGGGCAGCTTGCCGGGTGGCTGCGATGCGGTAGATAAAATCCCTGTGGCCCGCATTGTTGAA
GCACTGGAAAACCCTGGTGGCGCATATCAACACAACGGTATGGACCGACATTTTCCGGATATTCGTTTTATCTGGTGGGC
GGGCGGTGCCAACTTTACTCATCATCAGGATACCAACCGCCTGATCCGTGCCTGGCAAAAACCGGAGCTGGTGGTGATCT
CCGAATGTTTCTGGACGGCGGCGGCGAAACACGCGGATATCGTTCTGCCTGCGACCACCTCGTTTGAGCGTAATGATCTC
ACCATGACCGGCGATTACAGTAATCAGCATCTGGTGCCCATGAAGCAAGTGGTGCCGCCACGCTATGAAGCGCGTAATGA
TTTTGATGTTTTTGCCGAGTTAAGTGAACGCTGGGAGAAGGGCGGTTATGCACGTTTTACGGAAGGAAAAAGTGAGCTGC
AATGGCTGGAAACGTTTTATAACGTTGCCCGACAGCGCGGGGCAAGCCAGCAGGTTGAATTGCCGCCATTTGCTGAGTTC
TGGCAAGCCAACCAGTTAATTGAGATGCCGGAAAACCCGGACAGCGAGCGGTTTATTCGCTTCGCTGATTTTTGCCGCGA
TCCGCAGGCGCATCCATTAAAAACCGCCAGCGGTAAGATTGAAATCTTCTCGCAGCGTATTGCCGATTACGCTTATCCGG
ATTGCCCGGGGCATCCAATGTGGCTGGAGCCGGACGAATGGCAGGGCAATGCCGAACCAGAACAGTTGCAGGTACTTTCT
GCCCATCCGGCGCACCGCCTGCACAGCCAGCTGAATTACAGTTCTCTGCGCGAATTGTACGCGGTAGCAAACCGTGAGCC
TGTCACCATTCATCCTGACGATGCTCAGGCGCGCGGCATAACCGAAGGGGATATGGTGCGGGTGTGGAACTCCCGTGGGC
AGATCCTTGCCGGAGCGGTCATTAGCGAGGGAATTAAACCTGGCGTGATTTGCATTCACGAAGGGGCATGGCCGGATCTG
GATTTAACCGCTGACGGTATTTGTAAAAACGGCGCAGTGAACGTGCTGACCAAAGATCTCCCCAGCTCGCGGCTGGGGAA
TGGCTGTGCGGGTAATACGGCGCTGGCATGGCTGGAAAAATACAACGGTCCGGAACTGACACTTACAGCGTTTGAACCAC
CGGCCAGCTCATAA

Upstream 100 bases:

>100_bases
ATTATGCCGCGTGAACATGAAGATTATTCCTGGGAATACTCGGAAATTTGTAAGTAATATTTAACTGCTCAATACATCTA
ACCTTTCAGGAGTCTTCGGT

Downstream 100 bases:

>100_bases
TCCAGGTGGGTAGTTGGGTTTCATCCTGCCATGCGCAATCGACAATGTGAAAACCCTGCGCACGGTAAAAATCTATCGCC
GGTTGATTTTTTTGATAAAC

Product: biotin sulfoxide reductase

Products: Biotin. [C]

Alternate protein names: BDS reductase; BSO reductase [H]

Number of amino acids: Translated: 777; Mature: 776

Protein sequence:

>777_residues
MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQSAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQD
EFVRVSWDEALDLIHQQHKRIREAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVA
PHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYAVFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTM
LMAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDKIPVARIVE
ALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDL
TMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEF
WQANQLIEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEPDEWQGNAEPEQLQVLS
AHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDL
DLTADGICKNGAVNVLTKDLPSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS

Sequences:

>Translated_777_residues
MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQSAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQD
EFVRVSWDEALDLIHQQHKRIREAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVA
PHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYAVFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTM
LMAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDKIPVARIVE
ALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDL
TMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEF
WQANQLIEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEPDEWQGNAEPEQLQVLS
AHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDL
DLTADGICKNGAVNVLTKDLPSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS
>Mature_776_residues
ANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQSAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDE
FVRVSWDEALDLIHQQHKRIREAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVVG
GSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAP
HMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYAVFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTML
MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDKIPVARIVEA
LENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLT
MTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFW
QANQLIEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEPDEWQGNAEPEQLQVLSA
HPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLD
LTADGICKNGAVNVLTKDLPSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS

Specific function: This enzyme may serve as a scavenger, allowing the cell to utilize biotin sulfoxide as a biotin source. It reduces a spontaneous oxidation product of biotin, D-biotin D-sulfoxide (BSO or BDS), back to biotin [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI145693196, Length=777, Percent_Identity=98.8416988416988, Blast_Score=1604, Evalue=0.0,
Organism=Escherichia coli, GI87081994, Length=773, Percent_Identity=63.6481241914618, Blast_Score=1060, Evalue=0.0,
Organism=Escherichia coli, GI1787231, Length=790, Percent_Identity=42.9113924050633, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI87081797, Length=757, Percent_Identity=32.4966974900925, Blast_Score=309, Evalue=5e-85,
Organism=Escherichia coli, GI1787870, Length=753, Percent_Identity=30.9428950863214, Blast_Score=297, Evalue=2e-81,
Organism=Escherichia coli, GI171474008, Length=753, Percent_Identity=30.6772908366534, Blast_Score=280, Evalue=3e-76,
Organism=Escherichia coli, GI3868721, Length=474, Percent_Identity=23.6286919831224, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006658
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006655 [H]

Pfam domain/function: PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 85842; Mature: 85711

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQSAVRDQVHSNTRVRFP
CCCCCCCEEEEEEECCCCEEEECCCCCEECCCCCHHHCHHHHHHHHHHHHHHCCCEEEEH
MVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIREAYGPASIFAGSYGWRSN
HHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCC
GVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSD
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEECCHHHHHHCCCCCEEEECCC
VVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVA
EEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCHHHHC
PHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYAVFASYLLGESDGIAKTAEWAAEIC
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
GVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLS
CCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEE
YHFANGGNPTRRSAVLSSMQGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMDRHFPD
EEECCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
IRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDL
EEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCEEEECCCCCCCCCC
TMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSELQWLETFY
EEECCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
NVARQRGASQQVELPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPQAHPLKTASGKI
HHHHHCCCCCCCCCCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH
EIFSQRIADYAYPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELY
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHEEEECCCCHHHHHHHCCHHHHHHHH
AVANREPVTIHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDL
HHCCCCCEEECCCCHHHCCCCCCCEEEEECCCCCEEEHHHHHCCCCCCEEEEECCCCCCC
DLTADGICKNGAVNVLTKDLPSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS
CCCCCCCCCCCCCEEEECCCCCHHCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
ANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQSAVRDQVHSNTRVRFP
CCCCCCEEEEEEECCCCEEEECCCCCEECCCCCHHHCHHHHHHHHHHHHHHCCCEEEEH
MVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIREAYGPASIFAGSYGWRSN
HHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCC
GVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSD
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEECCHHHHHHCCCCCEEEECCC
VVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVA
EEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCHHHHC
PHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYAVFASYLLGESDGIAKTAEWAAEIC
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
GVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLS
CCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEE
YHFANGGNPTRRSAVLSSMQGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMDRHFPD
EEECCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
IRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDL
EEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCEEEECCCCCCCCCC
TMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSELQWLETFY
EEECCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
NVARQRGASQQVELPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPQAHPLKTASGKI
HHHHHCCCCCCCCCCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH
EIFSQRIADYAYPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELY
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHEEEECCCCHHHHHHHCCHHHHHHHH
AVANREPVTIHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDL
HHCCCCCEEECCCCHHHCCCCCCCEEEEECCCCCEEEHHHHHCCCCCCEEEEECCCCCCC
DLTADGICKNGAVNVLTKDLPSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS
CCCCCCCCCCCCCEEEECCCCCHHCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Spontaneous Oxidation Product Of Biotin, D-Biotin D-Sulfoxide (Bso Or Bds), [C]

Specific reaction: Reduces A Spontaneous Oxidation Product Of Biotin, D-Biotin D-Sulfoxide (Bso Or Bds), Back To Biotin. [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180922; 8041620; 9278503 [H]