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The map label for this gene is yhjJ

Identifier: 157163006

GI number: 157163006

Start: 3721756

End: 3723252

Strand: Reverse

Name: yhjJ

Synonym: EcHS_A3730

Alternate gene names: 157163006

Gene position: 3723252-3721756 (Counterclockwise)

Preceding gene: 157163007

Following gene: 157163004

Centisome position: 80.18

GC content: 53.44

Gene sequence:

>1497_bases
ATGCAGGGCACAAAAATTCGACTTTTAGCGGGCGGTTTGCTGATGATGGCCACTGCTGGCTATGTGCAGGCAGATGCGCT
CCAGCCTGATCCAGCATGGCAACAGGGGACGCTTTCCAACGGTTTACAGTGGCAAGTGCTGACCACCCCCCAGCGTCCCA
GCGATCGTGTTGAAATTCGCCTGCTGGTTAATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCC
ATCCCTCGTATTGCGCTAACGCAAAGCGGTGGCCTTGACGCAGCACAGGCGCGTTCATTGTGGCAGCAGGGGATCGACCC
TAAACGCCCGATGCCGCCGGTAATTGTCTCTTATGACACCACGCTGTTTAATCTGAGTTTGCCCAATAACCGTAACGATT
TGCTGAAAGAAGCGCTCTCTTATCTGGCAAATGCCACTGGCAAATTGACCATCACACCAGAAACCATCAACCACGCGCTG
CAAAGTCAGGACATGGTGGCAACCTGGCCTGCCGATACTAAAGAGGGCTGGTGGCGCTATCGTCTGAAAGGGTCAACCTT
GTTAGGTCACGATCCTGCCGATCCGCTGAAACAACCCGTTGAAGCGGAAAAAATTAAAGATTTCTATCAGAAATGGTACA
CCCCGGATGCAATGACGCTACTGGTGGTGGGAAACGTGGATGCGCGCTCGGTTGTCGACCAAATAAACAAAACGTTTGGC
GAACTGAAAGGCAAACGTGAAACGCCAGCTCCGGTGCCGACGCTTTCTCCGCTGCGTGCGGAAGCGGTGAGTATTATGAC
TGACGCGGTGCGTCAGGACCGGTTATCTATCATGTGGGATACGCCGTGGCAGCCGATTCGTGAATCAGCCGCACTGCTGC
GCTACTGGCGTGCGGACCTGGCCCGCGAGGCGCTGTTCTGGCATGTTCAGCAAGCGTTAAGTGCCAGTAACAGCAAAGAC
ATCGGTCTTGGATTTGACTGCCGTGTGCTGTATCTGCGTGCGCAGTGTGCCATCAACATCGAATCACCAAACGACAAGCT
GAACAGCAACCTTAATCTGGTGGCGCGTGAACTGGCGAAGGTTCGCGATAAAGGTCTGCCGGAAGAAGAGTTCAATGCGT
TAGTGGCGCAAAAGAAACTGGAGCTGCAGAAACTGTTTGCCGCCTATGCACGAGCTGATACCGATATTCTGATGGGTCAG
CGGATGCGTTCGTTGCAAAATCAGGTTGTCGATATCGCGCCGGAGCAATATCAGAAACTGCGGCAGGATTTCCTTAATAG
CCTGACGGTAGAGATGTTAAATCAGGATCTGCGTCAACAGTTGTCGAATGATATGGCGTTAATACTGCTGCAGCCGAAAG
GCGAGCCGGAATTTAACATGAAAGCGTTGCAGGCGGTCTGGGATCAAATCATGGCCCCATCTACCGCCGCTGCGACCACC
TCTGTCGCCACGGATGACGTACATCCTGAAGTGACGGATATTCCACCTGCACAGTAA

Upstream 100 bases:

>100_bases
TCATGTTCTTGCGGTCTAACACGAAGTGTTTTTAACGTCATTTAGACTGACCGACAACGGGCAGTTTTTTTATTACCAGG
ATAGTTGATCAGGGGTTCAC

Downstream 100 bases:

>100_bases
TGCCTGATGGTGATGAGTTAGCACAACCATCAGGTATTTATGGATGAGCTGGATAGAGGGGTAACACTTTATCCAGCCTT
TTGCATATGCTGCGTTTACG

Product: M16 family peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQWQVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHA
IPRIALTQSGGLDAAQARSLWQQGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAMTLLVVGNVDARSVVDQINKTFG
ELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLSIMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKD
IGLGFDCRVLYLRAQCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARADTDILMGQ
RMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILLQPKGEPEFNMKALQAVWDQIMAPSTAAATT
SVATDDVHPEVTDIPPAQ

Sequences:

>Translated_498_residues
MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQWQVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHA
IPRIALTQSGGLDAAQARSLWQQGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAMTLLVVGNVDARSVVDQINKTFG
ELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLSIMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKD
IGLGFDCRVLYLRAQCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARADTDILMGQ
RMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILLQPKGEPEFNMKALQAVWDQIMAPSTAAATT
SVATDDVHPEVTDIPPAQ
>Mature_498_residues
MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQWQVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHA
IPRIALTQSGGLDAAQARSLWQQGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAMTLLVVGNVDARSVVDQINKTFG
ELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLSIMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKD
IGLGFDCRVLYLRAQCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARADTDILMGQ
RMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILLQPKGEPEFNMKALQAVWDQIMAPSTAAATT
SVATDDVHPEVTDIPPAQ

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family

Homologues:

Organism=Escherichia coli, GI1789946, Length=498, Percent_Identity=100, Blast_Score=1016, Evalue=0.0,
Organism=Escherichia coli, GI1787770, Length=450, Percent_Identity=20.8888888888889, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YHJJ_ECOLI (P37648)

Other databases:

- EMBL:   U00039
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S47748
- RefSeq:   AP_004266.1
- RefSeq:   NP_417984.1
- ProteinModelPortal:   P37648
- SMR:   P37648
- IntAct:   P37648
- STRING:   P37648
- EnsemblBacteria:   EBESCT00000003064
- EnsemblBacteria:   EBESCT00000014919
- GeneID:   948040
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3495
- KEGG:   eco:b3527
- EchoBASE:   EB2164
- EcoGene:   EG12254
- eggNOG:   COG0612
- GeneTree:   EBGT00050000008280
- HOGENOM:   HBG417164
- OMA:   EVNERIQ
- ProtClustDB:   CLSK880707
- BioCyc:   EcoCyc:EG12254-MONOMER
- Genevestigator:   P37648
- GO:   GO:0006508
- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR007863
- Gene3D:   G3DSA:3.30.830.10

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C; SSF63411 Metalloenz_metal-bd

EC number: NA

Molecular weight: Translated: 55528; Mature: 55528

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00143 INSULINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQWQVLTTPQRPSDRVEIR
CCCCEEEEECCCHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
LLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQQGIDPKRPMPPVIVSYDT
EEEECCCHHHHHHHCCCHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECC
TLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHALQSQDMVATWPADTKEGWWRY
EEEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCEEEECCCCCCCCCEEE
RLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAMTLLVVGNVDARSVVDQINKTFG
EEECCEEECCCCCHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHH
ELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLSIMWDTPWQPIRESAALLRYWRADL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHH
AREALFWHVQQALSASNSKDIGLGFDCRVLYLRAQCAINIESPNDKLNSNLNLVARELAK
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHCCCHHHHHHHHHH
VRDKGLPEEEFNALVAQKKLELQKLFAAYARADTDILMGQRMRSLQNQVVDIAPEQYQKL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
RQDFLNSLTVEMLNQDLRQQLSNDMALILLQPKGEPEFNMKALQAVWDQIMAPSTAAATT
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHC
SVATDDVHPEVTDIPPAQ
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQWQVLTTPQRPSDRVEIR
CCCCEEEEECCCHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
LLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQQGIDPKRPMPPVIVSYDT
EEEECCCHHHHHHHCCCHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECC
TLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHALQSQDMVATWPADTKEGWWRY
EEEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCEEEECCCCCCCCCEEE
RLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAMTLLVVGNVDARSVVDQINKTFG
EEECCEEECCCCCHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHH
ELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLSIMWDTPWQPIRESAALLRYWRADL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHH
AREALFWHVQQALSASNSKDIGLGFDCRVLYLRAQCAINIESPNDKLNSNLNLVARELAK
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHCCCHHHHHHHHHH
VRDKGLPEEEFNALVAQKKLELQKLFAAYARADTDILMGQRMRSLQNQVVDIAPEQYQKL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
RQDFLNSLTVEMLNQDLRQQLSNDMALILLQPKGEPEFNMKALQAVWDQIMAPSTAAATT
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHC
SVATDDVHPEVTDIPPAQ
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 9298646