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The map label for this gene is gadX [H]

Identifier: 157162995

GI number: 157162995

Start: 3706297

End: 3707121

Strand: Reverse

Name: gadX [H]

Synonym: EcHS_A3719

Alternate gene names: 157162995

Gene position: 3707121-3706297 (Counterclockwise)

Preceding gene: 157162996

Following gene: 157162994

Centisome position: 79.83

GC content: 40.85

Gene sequence:

>825_bases
ATGCAATCACTACATGGGAATTGTCTAATTGCGTATGCAAGACATAAATATATTCTCACCATGGTTAATGGTGAATATCG
CTATTTTAATGGCGGTGACCTGGTTTTTGCGGATGCAAGCCAAATTCGAGTAGATAAGTGTGTTGAAAATTTTGTATTCG
TGTCAAGAGACACGCTTTCATTATTTCTCCCGATGCTCAAGGAGGAGGCATTAAATCTTCATGCACATAAAAAAGTTTCT
TCATTACTCGTTCATCACTGTAGTAGAGATATTCCTGTTTTTCAGGAAGTTGCGCAACTATCGCAGAATAAGAATCTTCG
CTATGCAGAAATGCTACGTAAAAGAGCATTAATCTTTGCGTTGTTATCTGTTTTTCTTGAGGATGAGCACTTTATACCGC
TGCTTCTGAACGTTTTACAACCGAACATGCGAACACGAGTTTGTACGGTTATCAATAATAATATCGCCCATGAGTGGACA
CTAGCCCGAATCGCCAGCGAGCTGTTGATGAGTCCAAGTCTGTTAAAGAAAAAATTGCGCGAAGAAGAGACATCATATTC
ACAGTTGCTTACTGAGTGTAGAATGCAACGTGCTTTGCAACTTATTGTTATACATGGTTTTTCAATTAAGCGAGTTGCAG
TATCCTGTGGATATCACAGCGTGTCGTATTTCATTTACGTCTTTCGAAATTATTATGGGATGACGCCCACAGAGTATCAG
GAGCGATCGGCGCAGAGATTGTCGAACCGTGACTCGGCGGCAAGTATTGTTGCGCAAGGGAATTTTTACGGCACTGACCG
TTCTGCGGAAGGAATAAGATTATAG

Upstream 100 bases:

>100_bases
CTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATGTT
TATGTAATATTAAGTCAACT

Downstream 100 bases:

>100_bases
AGTTTTACTCAGACATAAAAAAAACCCGGCATAGGGGACCGGGAAGAGGATAGTCTGCCGTCTCCAGACTAATAAACCGT
TATAACACTCCCTGTTGGCA

Product: DNA-binding transcriptional regulator GadX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVFVSRDTLSLFLPMLKEEALNLHAHKKVS
SLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQRLSNRDSAASIVAQGNFYGTDRSAEGIRL

Sequences:

>Translated_274_residues
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVFVSRDTLSLFLPMLKEEALNLHAHKKVS
SLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQRLSNRDSAASIVAQGNFYGTDRSAEGIRL
>Mature_274_residues
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVFVSRDTLSLFLPMLKEEALNLHAHKKVS
SLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
ERSAQRLSNRDSAASIVAQGNFYGTDRSAEGIRL

Specific function: Positively regulates the expression of about fifteen genes involved in acid resistance such as gadA, gadB and gadC. Depending on the conditions (growth phase and medium), can repress gadW. Negatively regulates perA expression in acidic conditions and posi

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789933, Length=274, Percent_Identity=100, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1790557, Length=228, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786778, Length=194, Percent_Identity=39.6907216494845, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787776, Length=121, Percent_Identity=45.4545454545455, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786776, Length=215, Percent_Identity=30.6976744186047, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789932, Length=209, Percent_Identity=32.5358851674641, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 31563; Mature: 31563

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVFVSRDTLS
CCCCCCCEEEEEECCEEEEEEECCCEEEECCCCEEEECHHHHHHHHHHHHHHHHCCHHHH
LFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPSLLKKKLR
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
ERSAQRLSNRDSAASIVAQGNFYGTDRSAEGIRL
HHHHHHHHCCCHHHHEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVFVSRDTLS
CCCCCCCEEEEEECCEEEEEEECCCEEEECCCCEEEECHHHHHHHHHHHHHHHHCCHHHH
LFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLRKRALIFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMSPSLLKKKLR
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQ
HHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
ERSAQRLSNRDSAASIVAQGNFYGTDRSAEGIRL
HHHHHHHHCCCHHHHEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA