Click here to switch to the map view.

The map label for this gene is garK

Identifier: 157162606

GI number: 157162606

Start: 3319245

End: 3320390

Strand: Reverse

Name: garK

Synonym: EcHS_A3313

Alternate gene names: 157162606

Gene position: 3320390-3319245 (Counterclockwise)

Preceding gene: 157162607

Following gene: 157162605

Centisome position: 71.51

GC content: 53.75

Gene sequence:

>1146_bases
ATGAAAATCGTAATCGCCCCAGACTCTTATAAAGAAAGTTTATCTGCCAGCGAGGTTGCGCAGGCGATAGAAAAAGGATT
TCGGGAAATTTTTCCTGATGCACAGTACGTTTCTGTTCCGGTTGCCGACGGTGGCGAAGGAACGGTGGAAGCGATGATTG
CAGCCACCCAGGGGGCTGAACGTCACGCCTGGGTTACAGGGCCGCTGGGCGAGAAAGTGAATGCCAGTTGGGGGATCTCC
GGCGATGGCAAAACCGCGTTTATTGAAATGGCGGCGGCCAGTGGGCTGGAGCTGGTACCTGCGGAAAAACGCGATCCACT
CGTGACCACTTCACGCGGCACAGGCGAGTTAATCCTGCAGGCGCTGGAGAGCGGTGCGACAAACATTATTATCGGCATTG
GCGGCAGCGCTACAAATGATGGCGGCGCAGGCATGGTACAGGCGCTGGGGGCGAAATTATGCGACGCCAACGGCAATGAA
ATTGGTTTTGGCGGCGGTAGTCTTAATACTCTGAATGATATTGATATTTCCGGCCTCGATCCGCGCTTAAAAGATTGCGT
CATTCGCGTCGCTTGTGATGTCACCAATCCGCTGGTGGGCGATAACGGCGCATCGCGCATCTTTGGCCCACAAAAGGGAG
CCAGTGAAGCGATGATTGTTGAGCTGGACAATAACCTCTCTCACTATGCCGAGGTCATTAAAAAAGCGCTGCATGTTGAT
GTGAAAGATGTCCCCGGTGCAGGAGCTGCGGGTGGTATGGGCGCGGCGCTAATGGCGTTTCTTGGTGCGGAACTGAAAAG
TGGTATTGAAATCGTCACTACGGCGCTGAATCTGGAGGAACATATTCACGATTGTACGCTGGTGATCACCGGTGAAGGGC
GTATTGACAGCCAGAGTATTCACGGGAAGGTACCGATTGGTGTCGCAAACGTGGCGAAGAAGTACCATAAACCGGTGATT
GGCATTGCGGGTAGCCTGACCGATGATGTTGGCGTTGTACATCAGCATGGCATTGATGCGGTCTTCAGCGTATTGACCAG
CATAGGTACGTTGGACGAAGCATTCCGCGGGGCTTATGACAATATCTGCCGTGCTTCACGTAATATCGCCGCGACACTGG
CGATTGGAATGCGCAACGCGGGGTGA

Upstream 100 bases:

>100_bases
TTAATGACGTCGTGCCCGGATGGCGTATTGCAATCCGGGCCTGGAATCCAGGCCGAATAAGAGAAACGCCCTCCGGCGTC
ATGTGGTAACAGGCATAGGT

Downstream 100 bases:

>100_bases
CAAGGGCGCGCAAACCCTCTATACTGCGCGCCGAAGCTGACCAGACAGTCGCCGCTTCGTCGTCGTCCTCTTCGGGGGAG
ACGGGCGGAGGGGAGGAAAG

Product: glycerate kinase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADGGEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGIS
GDGKTAFIEMAAASGLELVPAEKRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAMIVELDNNLSHYAEVIKKALHVD
VKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTALNLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVI
GIAGSLTDDVGVVHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG

Sequences:

>Translated_381_residues
MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADGGEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGIS
GDGKTAFIEMAAASGLELVPAEKRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAMIVELDNNLSHYAEVIKKALHVD
VKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTALNLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVI
GIAGSLTDDVGVVHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG
>Mature_381_residues
MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADGGEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGIS
GDGKTAFIEMAAASGLELVPAEKRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAMIVELDNNLSHYAEVIKKALHVD
VKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTALNLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVI
GIAGSLTDDVGVVHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG

Specific function: Unknown

COG id: COG1929

COG function: function code G; Glycerate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycerate kinase type-1 family

Homologues:

Organism=Escherichia coli, GI145693185, Length=381, Percent_Identity=100, Blast_Score=765, Evalue=0.0,
Organism=Escherichia coli, GI1786724, Length=376, Percent_Identity=56.3829787234042, Blast_Score=391, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLXK2_ECOLI (P23524)

Other databases:

- EMBL:   D90212
- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   JQ0614
- RefSeq:   AP_003670.1
- RefSeq:   NP_417593.4
- ProteinModelPortal:   P23524
- SMR:   P23524
- DIP:   DIP-28063N
- IntAct:   P23524
- STRING:   P23524
- PRIDE:   P23524
- EnsemblBacteria:   EBESCT00000001651
- EnsemblBacteria:   EBESCT00000016231
- GeneID:   947632
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3093
- KEGG:   eco:b3124
- EchoBASE:   EB1162
- EcoGene:   EG11175
- eggNOG:   COG1929
- GeneTree:   EBGT00050000009704
- HOGENOM:   HBG697930
- OMA:   QGATYTF
- ProtClustDB:   PRK10342
- BioCyc:   EcoCyc:GKI-MONOMER
- BioCyc:   MetaCyc:GKI-MONOMER
- Genevestigator:   P23524
- InterPro:   IPR018193
- InterPro:   IPR004381
- Gene3D:   G3DSA:3.90.1510.10
- PANTHER:   PTHR21599
- TIGRFAMs:   TIGR00045

Pfam domain/function: PF02595 Gly_kinase; SSF110738 Glyc_kinase

EC number: =2.7.1.31

Molecular weight: Translated: 39105; Mature: 39105

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADGGEGTVEAMIAATQGAE
CEEEECCCHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCC
RHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAEKRDPLVTTSRGTGELILQ
CCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCEEEECCCHHHHHHH
ALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNEIGFGGGSLNTLNDIDISGLD
HHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCC
PRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAMIVELDNNLSHYAEVIKKALHVD
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCC
VKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTALNLEEHIHDCTLVITGEGRIDSQSI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCEEEEEEECCCCCCCCCC
HGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVHQHGIDAVFSVLTSIGTLDEAFRGAYD
CCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHH
NICRASRNIAATLAIGMRNAG
HHHHHCCCEEEEEEEECCCCC
>Mature Secondary Structure
MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADGGEGTVEAMIAATQGAE
CEEEECCCHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCC
RHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAEKRDPLVTTSRGTGELILQ
CCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCEEEECCCHHHHHHH
ALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNEIGFGGGSLNTLNDIDISGLD
HHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCC
PRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAMIVELDNNLSHYAEVIKKALHVD
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCC
VKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTALNLEEHIHDCTLVITGEGRIDSQSI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCEEEEEEECCCCCCCCCC
HGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVHQHGIDAVFSVLTSIGTLDEAFRGAYD
CCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHH
NICRASRNIAATLAIGMRNAG
HHHHHCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1705543; 9278503