Click here to switch to the map view.

The map label for this gene is sdaA1

Identifier: 157162594

GI number: 157162594

Start: 3307123

End: 3308487

Strand: Reverse

Name: sdaA1

Synonym: EcHS_A3300

Alternate gene names: 157162594

Gene position: 3308487-3307123 (Counterclockwise)

Preceding gene: 157162595

Following gene: 157162593

Centisome position: 71.25

GC content: 53.41

Gene sequence:

>1365_bases
ATGATTAGTGCATTCGATATTTTCAAAATTGGGATTGGTCCCTCCAGTTCGCATACCGTGGGGCCAATGAATGCCGGAAA
AAGTTTTATTGATCGGCTGGAAAGTAGCGGCTTATTAACCGCGACGAGCCATATTGTGGTCGATCTGTACGGGTCGTTGT
CACTGACGGGCAAAGGCCATGCCACGGATGTCGCCATCATCATGGGACTGGCAGGAAACAGTCCGCAGGATGTTGTCATT
GATGAGATCCCTGCATTTATAGAGTTAGTAACGCGCAGCGGGCGGCTGCCAGTGGCATCTGGTGCGCATATTGTTGATTT
TCCTGTAGCAAAGAACATTATCTTCCATCCCGAAATGTTGCCTCGCCATGAGAACGGAATGCGGATCACTGCCTGGAAGG
GACAGGAAGAGCTATTAAGTAAAACCTATTACTCTGTCGGCGGCGGGTTTATTGTCGAAGAAGAACACTTCGGCCTGTCG
CACGATGTCGAAACGTCCGTACCTTACGATTTCCACTCAGCAGGTGAACTGCTGAAAATGTGTGATTACAACGGCCTGTC
TATATCTGGTCTGATGATGCACAACGAGCTAGCGCTGCGCAGCAAAGCGGAAATTGACGCCGGTTTTGCCCGTATCTGGC
AAGTGATGCATGACGGTATTGAACGTGGGATGAACACTGAAGGCGTGCTGCCTGGTCCGCTCAATGTGCCGCGCCGTGCC
GTAGCGCTGCGTCGTCAGCTGGTTTCCAGCGATAACATCTCTAACGATCCGATGAATGTCATCGACTGGATCAACATGTA
CGCGCTGGCGGTTAGTGAAGAAAACGCAGCTGGCGGGCGCGTGGTAACGGCACCGACTAACGGTGCGTGCGGCATTATTC
CGGCAGTACTGGCTTATTACGATAAGTTCCGTCGTCCGGTAAACGAGCGGTCAATTGCCCGCTATTTTCTGGCCGCGGGG
GCTATTGGCGCGCTGTATAAAATGAACGCCTCCATCTCTGGCGCGGAAGTCGGCTGTCAGGGGGAGATTGGCGTGGCCTG
TTCAATGGCGGCGGCAGGGTTAACTGAACTACTGGGCGGCAGTCCGGCGCAGGTATGCAATGCGGCGGAAATCGCGATGG
AGCATAACCTTGGGCTGACCTGCGATCCGGTTGCCGGACAGGTACAAATCCCGTGCATTGAACGTAATGCCATTAATGCC
GTGAAAGCAGTAAACGCCGCGCGGATGGCGATGCGCCGCACCTCGGCACCGCGTGTTTCACTCGATAAAGTGATCGAGAC
GATGTATGAAACCGGCAAAGATATGAACGATAAATACCGCGAAACATCACGCGGAGGACTGGCCATTAAAGTGGTCTGCG
GCTGA

Upstream 100 bases:

>100_bases
TTGAAGCCATCGCAGTACGTAGTGCGTAAAGCCTCGTGAGCGGGACGGTCGTAAGGTCGTTCCGCTCCACTTCACTGAAC
GGCAATCCGAGGGTGTGGAT

Downstream 100 bases:

>100_bases
CATTGCTGTCAGCTTTCATCCTTGGGTGCACAAATGTGCACCTTTTTTTTGTGATCTGCCGCAAATAGAGAATGAATATT
CTCTTGCGATTAATTTAGTA

Product: L-serine ammonia-lyase

Products: NA

Alternate protein names: SDH; L-serine deaminase

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSHIVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVI
DEIPAFIELVTRSGRLPVASGAHIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHDGIERGMNTEGVLPGPLNVPRRA
VALRRQLVSSDNISNDPMNVIDWINMYALAVSEENAAGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAG
AIGALYKMNASISGAEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPCIERNAINA
VKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIKVVCG

Sequences:

>Translated_454_residues
MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSHIVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVI
DEIPAFIELVTRSGRLPVASGAHIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHDGIERGMNTEGVLPGPLNVPRRA
VALRRQLVSSDNISNDPMNVIDWINMYALAVSEENAAGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAG
AIGALYKMNASISGAEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPCIERNAINA
VKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIKVVCG
>Mature_454_residues
MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSHIVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVI
DEIPAFIELVTRSGRLPVASGAHIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHDGIERGMNTEGVLPGPLNVPRRA
VALRRQLVSSDNISNDPMNVIDWINMYALAVSEENAAGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAG
AIGALYKMNASISGAEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPCIERNAINA
VKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIKVVCG

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family

Homologues:

Organism=Escherichia coli, GI48994925, Length=454, Percent_Identity=100, Blast_Score=934, Evalue=0.0,
Organism=Escherichia coli, GI1788116, Length=453, Percent_Identity=73.9514348785872, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1789161, Length=452, Percent_Identity=72.787610619469, Blast_Score=671, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TDCG_ECOLI (P42630)

Other databases:

- EMBL:   U18997
- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_003659.1
- RefSeq:   YP_026204.1
- ProteinModelPortal:   P42630
- SMR:   P42630
- DIP:   DIP-10974N
- STRING:   P42630
- EnsemblBacteria:   EBESCT00000000478
- EnsemblBacteria:   EBESCT00000016338
- GeneID:   2847724
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5520
- KEGG:   eco:b4471
- EchoBASE:   EB2610
- EcoGene:   EG12756
- eggNOG:   COG1760
- GeneTree:   EBGT00050000009684
- HOGENOM:   HBG304565
- OMA:   WKAQEEL
- ProtClustDB:   PRK15040
- BioCyc:   EcoCyc:LSERINEDEAM3-MONOMER
- BioCyc:   MetaCyc:LSERINEDEAM3-MONOMER
- Genevestigator:   P42630
- GO:   GO:0006094
- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131
- TIGRFAMs:   TIGR00720

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta

EC number: =4.3.1.17

Molecular weight: Translated: 48522; Mature: 48522

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSHIVVDLYGSLSLTGKGH
CCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCEEECCEEEEEEECCEEECCCCC
ATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGAHIVDFPVAKNIIFHPEML
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEECCCCC
PRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLSHDVETSVPYDFHSAGELLKM
CCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
CDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHDGIERGMNTEGVLPGPLNVPRRA
HCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
VALRRQLVSSDNISNDPMNVIDWINMYALAVSEENAAGGRVVTAPTNGACGIIPAVLAYY
HHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHH
DKFRRPVNERSIARYFLAAGAIGALYKMNASISGAEVGCQGEIGVACSMAAAGLTELLGG
HHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
SPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPCIERNAINAVKAVNAARMAMRRTSAPRVS
CHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCC
LDKVIETMYETGKDMNDKYRETSRGGLAIKVVCG
HHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure
MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSHIVVDLYGSLSLTGKGH
CCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCEEECCEEEEEEECCEEECCCCC
ATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGAHIVDFPVAKNIIFHPEML
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEECCCCC
PRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLSHDVETSVPYDFHSAGELLKM
CCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
CDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHDGIERGMNTEGVLPGPLNVPRRA
HCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
VALRRQLVSSDNISNDPMNVIDWINMYALAVSEENAAGGRVVTAPTNGACGIIPAVLAYY
HHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHH
DKFRRPVNERSIARYFLAAGAIGALYKMNASISGAEVGCQGEIGVACSMAAAGLTELLGG
HHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
SPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPCIERNAINAVKAVNAARMAMRRTSAPRVS
CHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCC
LDKVIETMYETGKDMNDKYRETSRGGLAIKVVCG
HHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 9484901