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The map label for this gene is yhaJ

Identifier: 157162589

GI number: 157162589

Start: 3302229

End: 3303125

Strand: Reverse

Name: yhaJ

Synonym: EcHS_A3295

Alternate gene names: 157162589

Gene position: 3303125-3302229 (Counterclockwise)

Preceding gene: 157162592

Following gene: 157162578

Centisome position: 71.13

GC content: 55.41

Gene sequence:

>897_bases
ATGGCCAAAGAAAGGGCATTAACGCTGGAAGCACTACGGGTTATGGATGCGATCGATCGCCGGGGCAGTTTTGCGGCGGC
GGCGGATGAGCTGGGACGCGTGCCTTCCGCACTTAGCTACACCATGCAAAAACTGGAAGAAGAGCTGGATGTGGTGCTGT
TTGACCGCTCGGGCCATCGTACCAAATTCACCAATGTCGGGAGGATGTTGCTGGAGCGAGGGCGCGTTTTGCTGGAAGCC
GCAGATAAACTGACTACTGATGCGGAAGCTCTCGCGCGCGGTTGGGAAACGCATCTCACCATTGTGACCGAAGCGCTGGT
ACCGACACCTGCCTTTTTCCCGTTAATCGACAAACTGGCGGCAAAAGCCAATACCCAACTGGCAATCATCACAGAAGTGC
TGGCGGGGGCGTGGGAACGGCTGGAGCAGGGGCGGGCGGATATTGTTATCGCGCCGGATATGCATTTTCGTTCCTCGTCG
GAGATCAACTCGCGCAAGCTCTATACGTTAATGAACGTCTACGTTGCCGCGCCTGATCACCCGATTCATCAGGAGCCGGA
ACCGTTATCTGAAGTGACGCGTGTGAAATATCGTGGAATTGCGGTGGCGGATACCGCTCGTGAGCGCCCGGTGTTGACCG
TACAGCTGCTGGACAAACAGCCGCGCTTAACGGTGAGCACGATTGAAGATAAACGTCAGGCATTACTGGCGGGGCTTGGC
GTGGCGACGATGCCGTATCCTATGGTCGAAAAAGATATTGCGGAAGGGCGGTTGCGTGTCGTCAGCCCGGAATCGACCAG
CGAGATCGATATTATTATGGCCTGGCGTCGTGACAGTATGGGGGAAGCGAAATCCTGGTGTCTGCGGGAAATTCCCAAAC
TTTTTAACGGAAAATAA

Upstream 100 bases:

>100_bases
CTTGACCTCTCTTTACTTCCTTTTATGGTAAGGGGCTGGACGCTACATTGTTAGCCAGATATTCTGCCCGGTATGTTCAA
ATTTCCTGAATGAGAACGAA

Downstream 100 bases:

>100_bases
TATTTATTATTGTTAATAAAAAGGGGCGAAATATCGCCCCAATTCTAAGATTAATTTGAACCAAACTTCGGATCGTTTCC
GTAACGATTGCTGCCAGAAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQKLEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEA
ADKLTTDAEALARGWETHLTIVTEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLG
VATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFNGK

Sequences:

>Translated_298_residues
MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQKLEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEA
ADKLTTDAEALARGWETHLTIVTEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLG
VATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFNGK
>Mature_297_residues
AKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQKLEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAA
DKLTTDAEALARGWETHLTIVTEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSSE
INSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGV
ATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFNGK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789492, Length=298, Percent_Identity=100, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI87082024, Length=286, Percent_Identity=38.4615384615385, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1787949, Length=280, Percent_Identity=29.6428571428571, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786508, Length=291, Percent_Identity=28.5223367697594, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786713, Length=281, Percent_Identity=26.6903914590747, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YHAJ_ECO57 (P67661)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91127
- RefSeq:   NP_289679.1
- RefSeq:   NP_312014.1
- ProteinModelPortal:   P67661
- SMR:   P67661
- EnsemblBacteria:   EBESCT00000028966
- EnsemblBacteria:   EBESCT00000060445
- GeneID:   916179
- GeneID:   958076
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4459
- KEGG:   ecs:ECs3987
- GeneTree:   EBGT00050000008332
- HOGENOM:   HBG685425
- OMA:   NVYVASP
- ProtClustDB:   CLSK869663
- BioCyc:   ECOL83334:ECS3987-MONOMER
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 33257; Mature: 33126

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQKLEEELDVVLFDRSGHR
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCCC
TKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIVTEALVPTPAFFPLIDKLA
CHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHH
AKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSSEINSRKLYTLMNVYVAAPDH
HHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHEEEEECCCC
PIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLG
CCCCCCCHHHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHCC
VATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFNGK
HHHCCCHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQKLEEELDVVLFDRSGHR
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCCC
TKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIVTEALVPTPAFFPLIDKLA
CHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHH
AKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSSEINSRKLYTLMNVYVAAPDH
HHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHEEEEECCCC
PIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLG
CCCCCCCHHHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHCC
VATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFNGK
HHHCCCHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796