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The map label for this gene is higA [H]

Identifier: 157162558

GI number: 157162558

Start: 3277072

End: 3277488

Strand: Reverse

Name: higA [H]

Synonym: EcHS_A3264

Alternate gene names: 157162558

Gene position: 3277488-3277072 (Counterclockwise)

Preceding gene: 157162559

Following gene: 157162550

Centisome position: 70.58

GC content: 48.92

Gene sequence:

>417_bases
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGCTCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Upstream 100 bases:

>100_bases
CAATGGTCTTTTTTGAATCGCAAAAGTGCTACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTT
CATCGTACTAAGGGGAAAAA

Downstream 100 bases:

>100_bases
CGCGACGTGCCGGATGCGTTGCATCCGGCGCTTCAAATAACGATTTAAATCTCCAGCGCCAGCCGTGTTCCCTGGGCAAT
TGCCCGTCGTGCGTCCAGCT

Product: helix-turn-helix DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 138; Mature: 138

Protein sequence:

>138_residues
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Sequences:

>Translated_138_residues
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID
>Mature_138_residues
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Specific function: Part of a toxin-antitoxin (TA) module. Functions as an mRNA interferase antitoxin [H]

COG id: COG5499

COG function: function code K; Predicted transcription regulator containing HTH domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789464, Length=138, Percent_Identity=99.2753623188406, Blast_Score=278, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 14952; Mature: 14952

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAW
CCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLE
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCHHHHHH
HAKKLATRFGISPALFID
HHHHHHHHHCCCCCEECC
>Mature Secondary Structure
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAW
CCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLE
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCHHHHHH
HAKKLATRFGISPALFID
HHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]