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The map label for this gene is prfB

Identifier: 157162351

GI number: 157162351

Start: 3062697

End: 3063717

Strand: Direct

Name: prfB

Synonym: EcHS_A3050

Alternate gene names: NA

Gene position: 3062697-3063717 (Clockwise)

Preceding gene: 157162349

Following gene: 157162355

Centisome position: 65.96

GC content: 54.06

Gene sequence:

>1099_bases
ATGTTTGAAATTAATCCGGTAAATAATCGCATTCAGGACCTCACGGAACGCTCCGACGTTCTTAGGGGGTATCTTTGACT
ACGACGCCAAGAAAGAGCGTCTGGAAGAAGTAAACGCCGAGCTGGAACAGCCGGATGTCTGGAACGAACCCGAACGCGCA
CAGGCGCTGGGTAAAGAGCGTTCCTCCCTCGAAGCCGTTGTCGACACCCTCGACCAAATGAAACAGGGGCTGGAAGATGT
TTCTGGTCTGCTGGAACTGGCTGTAGAAGCTGACGACGAAGAAACCTTTAACGAAGCCGTTGCTGAACTCGACGCCCTGG
AAGAAAAACTGGCGCAGCTTGAGTTCCGCCGTATGTTCTCTGGCGAATATGACAGCGCCGACTGCTACCTCGATATTCAG
GCGGGGTCTGGCGGTACGGAAGCACAGGACTGGGCGAGCATGCTTGAGCGTATGTATTTGCGCTGGGCAGAATCGCGTGG
TTTCAAAACTGAAATCATCGAAGAGTCGGAAGGTGAAGTGGCGGGTATTAAATCCGTGACGATCAAAATCTCCGGCGATT
ACGCTTACGGCTGGCTGCGTACAGAAACTGGCGTTCACCGCCTGGTGCGTAAGAGCCCGTTTGACTCCGGCGGTCGTCGC
CACACGTCGTTCAGCTCCGCGTTTGTTTATCCGGAAGTTGATGATGATATTGATATCGAAATCAACCCGGCGGATTTGCG
CATTGACGTTTATCGCGCGTCCGGCGCGGGCGGTCAGCACGTTAACCGTACCGAATCTGCGGTGCGTATTACCCACATCC
CGACCGGGATCGTGACCCAGTGCCAGAACGACCGTTCCCAGCACAAGAACAAAGACCAGGCCATGAAGCAGATGAAAGCG
AAGCTTTATGAACTGGAGATGCAGAAGAAAAATGCTGAGAAACAGGCGATGGAAGATAACAAATCTGACATCGGCTGGGG
CAGCCAGATTCGTTCTTATGTCCTTGATGACTCCCGCATTAAAGATCTGCGCACCGGGGTAGAAACCCGCAACACGCAGG
CCGTGCTGGACGGCAGCCTGGATCAATTTATCGAAGCAAGTTTGAAAGCAGGGTTATGA

Upstream 100 bases:

>100_bases
GCCAATTTAGCGTCATCTTCTCTATAAAAAAGAGCGTGGATTGGGTACAATCCCGCTCTTATCACCGCATTTTGACTAGC
TCAATAAAAGAAATCAGACC

Downstream 100 bases:

>100_bases
GGAACCAACATGTCTGAACAACACGCACAGGGCGCTGACGCGGTAGTCGATCTTAACAATGAACTGAAAACGCGTCGTGA
GAAGCTGGCGAACCTGCGTG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: None

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>365_residues
MFEINPVNNRIQDLTERSDVLRGYLCYDAKKERLEEVNAELEQPDVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDV
SGLLELAVEADDEETFNEAVAELDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFSSAFVYPEVDDDIDIEINPADLR
IDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQNDRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWG
SQIRSYVLDDSRIKDLRTGVETRNTQAVLDGSLDQFIEASLKAGL

Sequences:

>Translated_340_residues
S*PCFQTCFDKLIQAAVQHGLRVAGFYPGAQIFNAGVIKDIRTNLAAPADVRFVIFHRLFLSIFLLHLQFIKLRFHLLHG
LVFVLVLGTVVLALGHDPGRDVGNTHRRFGTVNVLTARAGRAINVNAQIRRVDFDINIIINFRINKRGAERRVATTAGVK
RALTHQAVNASFCTQPAVSVIAGDFDRHGFNTRHFTFRLFDDFSFETTRFCPAQIHTLKHARPVLCFRTARPRLNIEVAV
GAVIFAREHTAELKLRQFFFQGVEFSNGFVKGFFVVSFYSQFQQTRNIFQPLFHLVEGVDNGFEGGTLFTQRLCAFGFVP
DIRLFQLGVYFFQTLFLGVV
>Mature_340_residues
S*PCFQTCFDKLIQAAVQHGLRVAGFYPGAQIFNAGVIKDIRTNLAAPADVRFVIFHRLFLSIFLLHLQFIKLRFHLLHG
LVFVLVLGTVVLALGHDPGRDVGNTHRRFGTVNVLTARAGRAINVNAQIRRVDFDINIIINFRINKRGAERRVATTAGVK
RALTHQAVNASFCTQPAVSVIAGDFDRHGFNTRHFTFRLFDDFSFETTRFCPAQIHTLKHARPVLCFRTARPRLNIEVAV
GAVIFAREHTAELKLRQFFFQGVEFSNGFVKGFFVVSFYSQFQQTRNIFQPLFHLVEGVDNGFEGGTLFTQRLCAFGFVP
DIRLFQLGVYFFQTLFLGVV

Specific function: Unknown

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 38353; Mature: 38353

Theoretical pI: Translated: 11.21; Mature: 11.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
SPCFQTCFDKLIQAAVQHGLRVAGFYPGAQIFNAGVIKDIRTNLAAPADVRFVIFHRLFL
CCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHH
SIFLLHLQFIKLRFHLLHGLVFVLVLGTVVLALGHDPGRDVGNTHRRFGTVNVLTARAGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEEECCCC
AINVNAQIRRVDFDINIIINFRINKRGAERRVATTAGVKRALTHQAVNASFCTQPAVSVI
EEECCCEEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHH
AGDFDRHGFNTRHFTFRLFDDFSFETTRFCPAQIHTLKHARPVLCFRTARPRLNIEVAVG
HCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEEEEEE
AVIFAREHTAELKLRQFFFQGVEFSNGFVKGFFVVSFYSQFQQTRNIFQPLFHLVEGVDN
EEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GFEGGTLFTQRLCAFGFVPDIRLFQLGVYFFQTLFLGVV
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA