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The map label for this gene is ygdL

Identifier: 157162266

GI number: 157162266

Start: 2963245

End: 2964051

Strand: Reverse

Name: ygdL

Synonym: EcHS_A2956

Alternate gene names: 157162266

Gene position: 2964051-2963245 (Counterclockwise)

Preceding gene: 157162267

Following gene: 157162263

Centisome position: 63.83

GC content: 54.89

Gene sequence:

>807_bases
ATGTCTGTGGTAATTAGTGATGCATGGCGTCAGCGTTTTGGTGGCACAGCGCGTCTGTATGGTGAAAAAGCGTTGCAACT
GTTTGCTGACGCGCATATTTGTGTGGTCGGTATTGGCGGTGTCGGTTCCTGGGCGGCGGAAGCGCTGGCGCGCACGGGGA
TTGGCGCAATCACGCTTATCGATATGGATGATGTGTGCGTCACCAATACCAATCGGCAAATTCATGCCCTGCGCGATAAC
GTTGGGCTGGCAAAAGCGGAAGTCATGGCGGAACGTATTCGCCAGATTAACCCGGAGTGCCGTGTAACGGTGGTGGATGA
TTTCGTGACGCCGGATAACGTAGCGCAGTATATGAGCGTGGGTTATTCGTACGTGATTGATGCCATTGATAGTGTACGGC
CCAAAGCGGCGCTGATTGCTTATTGTCGGCGCAATAAAATCCCGCTGGTCACGACCGGTGGCGCGGGTGGGCAGATTGAC
CCGACGCAGATTCAGGTTACCGATCTGGCGAAAACGATTCAGGATCCGCTGGCGGCGAAGTTGCGCGAGCGCCTGAAAAG
CGATTTTGGCGTAGTGAAAAACAGTAAAGGTAAGCTCGGCGTGGATTGCGTGTTTTCTACTGAAGCGCTGGTGTACCCGC
AGTCAGACGGTACGGTGTGTGCGATGAAAGCCACGGCAGAAGGGCCAAAGCGGATGGATTGTGCATCTGGATTTGGCGCG
GCAACGATGGTGACCGCCACCTTTGGGTTTGTTGCGGTTTCTCATGCGCTGAAGAAGATGATGGCGAAAGCGGCGCGTCA
GGGTTAA

Upstream 100 bases:

>100_bases
AAATATTGCAGGAGTTATGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTATGCGTTTAGGGTGAGGGATAACCTCGCC
CTTTTTAATTCTGAGGTGCT

Downstream 100 bases:

>100_bases
ACAATCAGGTCGGATGAGACGCGAACGTCTTATCCGACCCGGTTCTGTGCTTAAACCTGCTTCGTCGCAGCGATAATCGC
CTCGCTTAACGCATTTAACC

Product: ThiF family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSWAAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDN
VGLAKAEVMAERIRQINPECRVTVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGTVCAMKATAEGPKRMDCASGFGA
ATMVTATFGFVAVSHALKKMMAKAARQG

Sequences:

>Translated_268_residues
MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSWAAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDN
VGLAKAEVMAERIRQINPECRVTVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGTVCAMKATAEGPKRMDCASGFGA
ATMVTATFGFVAVSHALKKMMAKAARQG
>Mature_267_residues
SVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSWAAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNV
GLAKAEVMAERIRQINPECRVTVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQIDP
TQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGTVCAMKATAEGPKRMDCASGFGAA
TMVTATFGFVAVSHALKKMMAKAARQG

Specific function: Unknown

COG id: COG1179

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family

Homologues:

Organism=Homo sapiens, GI4885649, Length=143, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789177, Length=268, Percent_Identity=100, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI87082356, Length=136, Percent_Identity=30.1470588235294, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787048, Length=131, Percent_Identity=32.824427480916, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17540406, Length=131, Percent_Identity=31.2977099236641, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6321790, Length=205, Percent_Identity=24.8780487804878, Blast_Score=92, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6322825, Length=202, Percent_Identity=31.1881188118812, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24582879, Length=132, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24660640, Length=137, Percent_Identity=29.9270072992701, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YGDL_ECOLI (Q46927)

Other databases:

- EMBL:   U29581
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H65063
- RefSeq:   AP_003378.1
- RefSeq:   NP_417292.1
- ProteinModelPortal:   Q46927
- SMR:   Q46927
- STRING:   Q46927
- EnsemblBacteria:   EBESCT00000002841
- EnsemblBacteria:   EBESCT00000015495
- GeneID:   947291
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2783
- KEGG:   eco:b2812
- EchoBASE:   EB2893
- EcoGene:   EG13084
- eggNOG:   COG1179
- GeneTree:   EBGT00050000010430
- HOGENOM:   HBG321303
- OMA:   DPTQIQI
- ProtClustDB:   PRK15116
- BioCyc:   EcoCyc:G7456-MONOMER
- Genevestigator:   Q46927
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF00899 ThiF; SSF69572 MoeB

EC number: NA

Molecular weight: Translated: 28562; Mature: 28431

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x16c9f338)-;

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSWAAEALARTGIGAITLI
CEEEECHHHHHHHCCCEEHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEEE
DMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRVTVVDDFVTPDNVAQYMSV
ECCCEEEECCCCEEEEHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
GYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQIDPTQIQVTDLAKTIQDPLAAK
HHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHH
LRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGTVCAMKATAEGPKRMDCASGFGA
HHHHHHHHHCCEECCCCCCCEEEEEECCEEEEECCCCCEEEEEECCCCCCCCHHHCCCCH
ATMVTATFGFVAVSHALKKMMAKAARQG
HHHHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
SVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSWAAEALARTGIGAITLI
EEEECHHHHHHHCCCEEHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEEE
DMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRVTVVDDFVTPDNVAQYMSV
ECCCEEEECCCCEEEEHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
GYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQIDPTQIQVTDLAKTIQDPLAAK
HHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHH
LRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGTVCAMKATAEGPKRMDCASGFGA
HHHHHHHHHCCEECCCCCCCEEEEEECCEEEEECCCCCEEEEEECCCCCCCCHHHCCCCH
ATMVTATFGFVAVSHALKKMMAKAARQG
HHHHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503