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The map label for this gene is sdaB [H]

Identifier: 157162251

GI number: 157162251

Start: 2947785

End: 2949152

Strand: Direct

Name: sdaB [H]

Synonym: EcHS_A2941

Alternate gene names: 157162251

Gene position: 2947785-2949152 (Clockwise)

Preceding gene: 157162250

Following gene: 157162252

Centisome position: 63.48

GC content: 54.39

Gene sequence:

>1368_bases
ATGATTAGCGTATTCGATATTTTCAAAATCGGCATTGGCCCTTCCAGTTCTCATACCGTTGGACCAATGAAAGCGGGTAA
ACAATTTACCGACGATCTGATTGCCCATAATCTGCTTAAAGACGTGACCCGCGTGGTGGTTGACGTGTACGGCTCGCTCT
CTCTGACCGGTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATC
GATTCCATCCCTGGTTTTATTCAGGATGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTT
CCCGGTTGATCAGTGCATGAACTTCCATGCAGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGG
GCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAG
AATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAACCGGGCTGTC
ACTCTCTGGCCTGATGATGAAAAATGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTATGGG
AAGTCATGCGTGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCT
GCGGCACTACGCCGGATGCTGGTCAGCCAGGATAAAACCACCACTGACCCGATGGCGGTTGTTGACTGGATCAACATGTT
TGCACTGGCAGTGAACGAAGAGAACGCTGCTGGCGGACGCGTGGTGACTGCGCCGACTAACGGTGCGTGCGGGATTATCC
CGGCAGTGCTGGCGTACTACGACAAGTTTATCCGCGAAGTGAACGCTAACTCACTGGCTCGTTACCTGCTGGTAGCCAGT
GCCATTGGTTCTCTTTATAAGATGAACGCGTCAATTTCTGGTGCTGAAGTCGGCTGCCAGGGTGAAGTTGGTGTGGCGTG
CTCAATGGCGGCGGCTGGTCTGGCAGAGCTGTTAGGTGCAAGCCCGGCGCAGGTGTGCATCGCGGCGGAAATCGCCATGG
AGCACAACCTCGGTCTGACGTGTGACCCGGTCGCCGGACAGGTCCAGGTGCCATGCATCGAGCGTAACGCCATTGCGGCA
GTAAAAGCGGTGAACGCCGCACGTATGGCGCTACGCCGTACCAGCGAGCCGCGCGTCTGCCTCGATAAAGTTATCGAAAC
CATGTACGAAACAGGTAAAGATATGAACGCCAAGTACCGCGAAACCTCTCGCGGCGGCCTGGCAATGAAGATCGTTGCCT
GCGATTAA

Upstream 100 bases:

>100_bases
TCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGT
TTTAACGCGATGTATTTCCT

Downstream 100 bases:

>100_bases
TCGCTCTCCAGAGGCCTCGTTTTGCGAGGCCTCTTCCCGATTTCTCATCCAGCCGTAGCCTGTTCCGGCATCGAATGTTA
CCCTTATCGCCTGATCTTTA

Product: L-serine ammonia-lyase

Products: NA

Alternate protein names: SDH 2; L-serine deaminase 2; L-SD2 [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIAHNLLKDVTRVVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDI
DSIPGFIQDVNTHGRLMLANGQHEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRGGIERGISTEGVLPGKLRVPRRA
AALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENAAGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVAS
AIGSLYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPCIERNAIAA
VKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMKIVACD

Sequences:

>Translated_455_residues
MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIAHNLLKDVTRVVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDI
DSIPGFIQDVNTHGRLMLANGQHEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRGGIERGISTEGVLPGKLRVPRRA
AALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENAAGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVAS
AIGSLYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPCIERNAIAA
VKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMKIVACD
>Mature_455_residues
MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIAHNLLKDVTRVVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDI
DSIPGFIQDVNTHGRLMLANGQHEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRGGIERGISTEGVLPGKLRVPRRA
AALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENAAGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVAS
AIGSLYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPCIERNAIAA
VKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMKIVACD

Specific function: Deaminates also threonine, particularly when it is present in high concentration [H]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI1789161, Length=455, Percent_Identity=99.3406593406593, Blast_Score=934, Evalue=0.0,
Organism=Escherichia coli, GI1788116, Length=455, Percent_Identity=77.5824175824176, Blast_Score=736, Evalue=0.0,
Organism=Escherichia coli, GI48994925, Length=452, Percent_Identity=72.787610619469, Blast_Score=671, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 48704; Mature: 48704

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIAHNLLKDVTRVVVDVYGSLSLTGKGH
CCCCEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCC
HTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQHEVEFPVDQCMNFHADNL
CCCEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCEECCHHHHHCCCCCCC
SLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQNSAPVEVPYPYSSAADLQKH
EEECCCEEEEEEECCEEEEEEHEEECCCCEEEEHHHCCCCCCCCEEECCCCCCHHHHHHH
CQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRGGIERGISTEGVLPGKLRVPRRA
HHHHCCCEEHEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
AALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENAAGGRVVTAPTNGACGIIPAVLAYY
HHHHHHHHHCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHH
DKFIREVNANSLARYLLVASAIGSLYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGA
HHHHHHHCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
SPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPCIERNAIAAVKAVNAARMALRRTSEPRVC
CHHHHHHHHHHHHHCCCCCEECCCCCCEECCEECCCHHHHHHHHHHHHHHHHCCCCCHHH
LDKVIETMYETGKDMNAKYRETSRGGLAMKIVACD
HHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEEECC
>Mature Secondary Structure
MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIAHNLLKDVTRVVVDVYGSLSLTGKGH
CCCCEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCC
HTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQHEVEFPVDQCMNFHADNL
CCCEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCEECCHHHHHCCCCCCC
SLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQNSAPVEVPYPYSSAADLQKH
EEECCCEEEEEEECCEEEEEEHEEECCCCEEEEHHHCCCCCCCCEEECCCCCCHHHHHHH
CQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRGGIERGISTEGVLPGKLRVPRRA
HHHHCCCEEHEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
AALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENAAGGRVVTAPTNGACGIIPAVLAYY
HHHHHHHHHCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHH
DKFIREVNANSLARYLLVASAIGSLYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGA
HHHHHHHCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
SPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPCIERNAIAAVKAVNAARMALRRTSEPRVC
CHHHHHHHHHHHHHCCCCCEECCCCCCEECCEECCCHHHHHHHHHHHHHHHHCCCCCHHH
LDKVIETMYETGKDMNAKYRETSRGGLAMKIVACD
HHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8385012; 9278503 [H]