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The map label for this gene is hycG

Identifier: 157162165

GI number: 157162165

Start: 2860861

End: 2861628

Strand: Reverse

Name: hycG

Synonym: EcHS_A2855

Alternate gene names: 157162165

Gene position: 2861628-2860861 (Counterclockwise)

Preceding gene: 157162166

Following gene: 157162164

Centisome position: 61.63

GC content: 56.38

Gene sequence:

>768_bases
ATGAGCAATTTATTAGGCCCCCGTGACGCCAACGGCATTCCGGTCCCCATGACGGTGGATGAATCCATCGCCAGCATGAA
GGCGTCGTTACTGAAAAAAATCAAACGTTCTGCCTATGTTTACCGCGTGGACTGCGGCGGCTGCAACGGTTGCGAAATCG
AAATTTTCGCCACTTTATCGCCGCTGTTTGATGCAGAACGCTTCGGCATTAAAGTCGTTCCTTCACCGCGTCATGCGGAT
ATTTTACTGTTTACCGGCGCGGTCACCCGTGCAATGCGATCCCCTGCGCTGCGTGCGTGGCAGTCCGCGCCGGACCCGAA
AATTTGTATCTCCTACGGTGCCTGCGGTAACAGCGGCGGGATCTTCCACGATCTCTACTGCGTGTGGGGCGGTACGGATA
AAATTGTCCCTGTGGATGTTTATATCCCTGGCTGCCCGCCAACGCCTGCCGCCACGCTGTACGGCTTTGCAATGGCGCTC
GGCCTGCTGGAGCAGAAAATTCACGCCCGTGGGCCGGGTGAACTGGATGAACAACCGGCGGAGATCCTGCATGGCGATAT
GGTGCAGCCGCTGCGCGTGAAAGTGGATCGCGAAGCACGTCGCCTGGCGGGTTATCGTTACGGTCGTCAGATTGCCGATG
ATTACCTTACACAGTTAGGGCAGGGCGAAGAACAGGTTGCACGCTGGCTGGAAGCGGAAAACGATCCGCGTCTGAACGAG
ATTGTCAGCCATCTGAATCATGTTGTTGAAGAGGCGCGTATCCGATGA

Upstream 100 bases:

>100_bases
ACCGCGAAAGCTTTGAGACTTGCCCGGAATGTAAGCGCCAGAAATGCCTGGTGCCGTCCGACCGTATTGAACTGACTCGC
CATATGAAAGAGGCCATCTG

Downstream 100 bases:

>100_bases
GTGAAAAGGTGGTGTTCAGTCAACTGAGCCGTAAATTTATTGATGAGAACGATGCCACGCCCGCCGAGGCGCAGCAGGTG
GTCTATTACAGCCTGGCGAT

Product: formate hydrogenlyase, subunit G

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVDCGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHAD
ILLFTGAVTRAMRSPALRAWQSAPDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQLGQGEEQVARWLEAENDPRLNE
IVSHLNHVVEEARIR

Sequences:

>Translated_255_residues
MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVDCGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHAD
ILLFTGAVTRAMRSPALRAWQSAPDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQLGQGEEQVARWLEAENDPRLNE
IVSHLNHVVEEARIR
>Mature_254_residues
SNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVDCGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADI
LLFTGAVTRAMRSPALRAWQSAPDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMALG
LLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQLGQGEEQVARWLEAENDPRLNEI
VSHLNHVVEEARIR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=111, Percent_Identity=44.1441441441441, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1789074, Length=255, Percent_Identity=100, Blast_Score=526, Evalue=1e-151,
Organism=Escherichia coli, GI1788834, Length=251, Percent_Identity=62.1513944223108, Blast_Score=309, Evalue=1e-85,
Organism=Escherichia coli, GI1788624, Length=157, Percent_Identity=36.9426751592357, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=130, Percent_Identity=40, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI18859983, Length=110, Percent_Identity=42.7272727272727, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24642371, Length=110, Percent_Identity=42.7272727272727, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24651058, Length=110, Percent_Identity=43.6363636363636, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYCG_ECOLI (P16433)

Other databases:

- EMBL:   X17506
- EMBL:   U29579
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S08625
- RefSeq:   AP_003286.1
- RefSeq:   NP_417199.1
- ProteinModelPortal:   P16433
- SMR:   P16433
- DIP:   DIP-9977N
- IntAct:   P16433
- MINT:   MINT-1287919
- STRING:   P16433
- EnsemblBacteria:   EBESCT00000004153
- EnsemblBacteria:   EBESCT00000017104
- GeneID:   947191
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2689
- KEGG:   eco:b2719
- EchoBASE:   EB0475
- EcoGene:   EG10480
- eggNOG:   COG3260
- GeneTree:   EBGT00050000009829
- HOGENOM:   HBG553221
- OMA:   TRAMRVP
- ProtClustDB:   CLSK880465
- BioCyc:   EcoCyc:HYCG-MONOMER
- BioCyc:   MetaCyc:HYCG-MONOMER
- Genevestigator:   P16433
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995

Pfam domain/function: PF01058 Oxidored_q6

EC number: NA

Molecular weight: Translated: 27999; Mature: 27868

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVDCGGCNGCEIEIFATLS
CCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEECC
PLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQSAPDPKICISYGACGNSGG
HHHHHHHCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
IFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMALGLLEQKIHARGPGELDEQPA
CEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
EILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQLGQGEEQVARWLEAENDPRLNE
HHHHCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
IVSHLNHVVEEARIR
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVDCGGCNGCEIEIFATLS
CCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEECC
PLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQSAPDPKICISYGACGNSGG
HHHHHHHCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
IFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMALGLLEQKIHARGPGELDEQPA
CEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
EILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQLGQGEEQVARWLEAENDPRLNE
HHHHCCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
IVSHLNHVVEEARIR
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503