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The map label for this gene is ascG [H]

Identifier: 157162160

GI number: 157162160

Start: 2855672

End: 2856685

Strand: Reverse

Name: ascG [H]

Synonym: EcHS_A2850

Alternate gene names: 157162160

Gene position: 2856685-2855672 (Counterclockwise)

Preceding gene: 157162163

Following gene: 157162159

Centisome position: 61.52

GC content: 53.16

Gene sequence:

>1014_bases
ATGATGACGACGATGCTGGAAGTGGCGAAGCGCGCCGGGGTTTCAAAAGCGACCGTTTCCCGCGTGCTTTCAGGTAATGG
CTACGTCAGCCAGGAGACTAAAGATCGCGTGTTTCAGGCGGTAGAAGAGAGCGGTTACCGTCCAAACTTGCTGGCGCGCA
ATCTGTCGGCGAAGAGTACTCAGACGCTGGGGCTGGTAGTGACCAACACGCTTTACCACGGCATTTATTTTAGTGAATTA
CTGTTTCATGCCGCGCGAATGGCGGAAGAGAAAGGGCGACAGTTGCTATTGGCAGATGGTAAACACAGCGCCGAAGAAGA
GCGCCAGGCGATTCAGTATCTGCTGGATTTGCGCTGCGACGCCATTATGATTTACCCGCGTTTTTTAAGCGTGGATGAGA
TCGATGACATCATTGACGCGCACAGTCAGCCGATAATGGTGCTTAATCGCCGCCTGCGCAAAAATAGCAGCCATAGCGTC
TGGTGCGATCATAAACAGACCAGCTTTAACGCCGTGGCAGAGTTGATAAACGCCGGGCATCAGGAGATTGCTTTCCTTAC
CGGCTCGATGGATTCCCCCACCAGCATTGAACGTCTTGCCGGGTATAAAGACGCGCTGGCGCAGCATGGTATTGCGCTCA
ATGAACAACTTATCGCTAACGGTAAATGGACGCCCGCCAGCGGGGCCGAAGGGGTAGAAACGTTGCTCGAACGTGGGGCT
AAATTTAGCGCGTTAGTTGCCAGTAACGACGATATGGCGATAGGTGCGATGAAAGCGTTGCACGAGCGCGGCGTAGCGGT
GCCAGAGCAGGTGTCAGTTATCGGATTCGATGATATCGCAATTGCCCCCTATATCGTTCCGGCGCTCTCCAGCGTAAAAA
TTCCGGTGACTGAGATGATTCAGGAAATTATTGGACGGCTGATTTTTATGCTCGATGGGGGCGCTTACACACCGCCGAAA
ACCTTCAGCGGAAAACTGATCCGCCGCGACTCACTCATTGCTCTTTCGCGATAA

Upstream 100 bases:

>100_bases
TTTGTGAAACCGGTCACCTGAATATTTTTCCCGCCGATTGTGCAAGGACAATCGGCAAACGGTGCAGTACACTGCGTTGA
TTGCGTGTAAAGGGATGAAA

Downstream 100 bases:

>100_bases
ACATTGTCATCACATGTCATCGTCACTAGTGTCGATGACATCTTTTTTCCTGCATTACAGAAATTAATTTCCTTAAATAA
CAGTAAATTAAAAACTGGCA

Product: transcriptional regulator AscG

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSEL
LFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSV
WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEQLIANGKWTPASGAEGVETLLERGA
KFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPK
TFSGKLIRRDSLIALSR

Sequences:

>Translated_337_residues
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSEL
LFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSV
WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEQLIANGKWTPASGAEGVETLLERGA
KFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPK
TFSGKLIRRDSLIALSR
>Mature_337_residues
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSEL
LFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSV
WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEQLIANGKWTPASGAEGVETLLERGA
KFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPK
TFSGKLIRRDSLIALSR

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=97.9228486646884, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=33.6336336336336, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=35.3293413173653, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1787948, Length=301, Percent_Identity=30.2325581395349, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1790369, Length=311, Percent_Identity=30.8681672025724, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=28.7925696594427, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790194, Length=307, Percent_Identity=27.6872964169381, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1786540, Length=337, Percent_Identity=29.080118694362, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790715, Length=295, Percent_Identity=25.4237288135593, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787906, Length=284, Percent_Identity=29.9295774647887, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=25.5520504731861, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789456, Length=303, Percent_Identity=26.7326732673267, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786268, Length=304, Percent_Identity=24.6710526315789, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36929; Mature: 36929

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKST
CCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
QTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
AIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVWCDHKQTSFNAVAELINAGH
EEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCC
QEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEQLIANGKWTPASGAEGVETLLERGA
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCC
KFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMI
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHH
QEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIALSR
HHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEECC
>Mature Secondary Structure
MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESGYRPNLLARNLSAKST
CCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
QTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSAEEERQAIQYLLDLRCD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
AIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVWCDHKQTSFNAVAELINAGH
EEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCC
QEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEQLIANGKWTPASGAEGVETLLERGA
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCC
KFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIAPYIVPALSSVKIPVTEMI
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHH
QEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIALSR
HHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]