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The map label for this gene is hypF [H]

Identifier: 157162158

GI number: 157162158

Start: 2852591

End: 2854843

Strand: Reverse

Name: hypF [H]

Synonym: EcHS_A2848

Alternate gene names: 157162158

Gene position: 2854843-2852591 (Counterclockwise)

Preceding gene: 157162159

Following gene: 157162155

Centisome position: 61.48

GC content: 57.97

Gene sequence:

>2253_bases
ATGGCAAAAAACACATCTTGCGGTGTCCAACTGCGTATTCGTGGCAAAGTGCAGGGCGTCGGTTTTCGTCCGTTTGTCTG
GCAGCTGGCACAGCAATTAAATCTTCACGGCGATGTCTGTAATGACGGCGATGGCGTAGAAGTCCGGCTGCTGGAAGACC
CGGAAACGTTTCTTGTTCAATTGCATCAGCACTGTCCGCCACTGGCGCGTATTGATAGCGTCGAGCGTGAGCCGTTTATC
TGGTCACAACTGCCCACCGAGTTCACTATCCGCCAGAGCGCGGGCGGTGCCATGAATACGCAAATTGTCCCGGATGCTGC
CACTTGCCATGCTTGCCTTGCCGAAATGAATACCCCAGGCGAACGGCGTTATCGTTATCCGTTTATCAACTGTACCCACT
GCGGCCCGCGCTTCACCATTATTCGCGCCATGCCTTACGACCGCCCGTTTACCGTGATGGCGGCGTTTCCGCTGTGTCCG
GCCTGTGATAAAGAGTACCGTGACCCGCTCGATCGTCGCTTCCACGCCCAGCCGGTGGCCTGCCCGGAGTGTGGCCCGCA
TCTTGAATGGGTAAGTCATGGTGAACATGCAGAACAAGAGGCGGCATTACAGGCAGCTATCGCACAGTTAAAAATGGGCA
ACATTGTCGCCATCAAAGGGATTGGCGGATTTCATCTTGCCTGCGATGCACGTAACAGTAACGCGGTGGCGACACTGCGG
GCACGCAAACATCGCCCGGCGAAACCGCTGGCGGTTATGTTGCCAGTGGCAGAAGGTTTACCAGACGCTGCGCGCCAGTT
GCTTACCACGCCCGCCGCGCCGATTGTGCTGGTGGATAAAAAATACGTTCCTGAGCTTTGTGATGATATCGCCCCTGGCC
TTAACGAAGTCGGGGTGATGTTGCCTGCGAACCCGCTCCAGCATTTGCTGTTACAGGAACTGCAATGCCCGCTGGTGATG
ACCTCCGGCAACCTGAGCGGTAAACCACCGGCTATCAGCAACGAACAGGCGCTGGCGGATTTGCAGGGCATTGCCGACGG
ATTCTTGATACATAACCGCGACATCGTGCAGCGGATGGATGATTCGGTGGTGCGCGAAAGCGGCGAAATGCTGCGCCGTT
CGCGGGGGTATGTGCCGGATGCGCTGGCTTTGCCTCTGGGCTTTAAAAATGTTCCGCCTGTGCTGTGTCTCGGCGCGGAT
CTGAAAAACACCTTCTGCCTGGTGCGCGGTGAACAAGCGGTGTTGAGTCAGCATCTGGGCGATTTAAGTGACGATGGCAT
CCAGATGCAGTGGCGCGAAGCGTTACGCCTGATGCAAAACATCTACGATTTCACTCCGCAATACGTTGTGCATGACGCGC
ATCCGGGCTATGTCTCCAGCCAGTGGGCGCGCGAAATGAATCTGCCGACGCAAACGGTGCTGCATCATCATGCCCACGCA
GCGGCGTGTCTGGCAGAGCATCAGTGGCCGCTGGATGGCGGTGATGTCATTGCTTTGACGCTCGACGGTATCGGTATGGG
GGAGAACGGCGCTTTGTGGGGCGGCGAGTGCCTGCGGGTGAACTATCGCGAATGCCAGCACCTGGGCGGCTTGCCTGCGG
TGGCGCTTCCGGGTGGCGATTTGGCAGCGAAGCAGCCGTGGCGAAACCTGCTGGCGCAGTGCCTGCGCTTTGTGCCGGAG
TGGCAGAATTACTCTGAAACAGCAAGTGTGCAACAGCAAAACTGGAGCGTGCTGGCGCGGGCCATTGAGCGTGGAATTAA
CGCGCCGCTGGCGTCATCGTGTGGGCGTTTTTTCGATGCAGTGGCGGCGGCACTGGGCTGTGCGCCAGCCACGTTAAGTT
ATGAAGGTGAAGCGGCTTGTGCTCTGGAGGCGCTCGCAGCCTCATGCCACGGAGTGACGCATCCGGTGACAATGCCGCGG
GTGGACAATCAACTGGATCTCGCCACTTTCTGGCAGCAGTGGCTGAACTGGCAGGCACCGGTTAATCAACGCGCGTGGGC
GTTTCATGATGCGCTGGCGCAGGGTTTTGCCGCGTTGATGCGTGAGCAGGCCACGATGCGTGGTATCACTACGCTGGTAT
TTAGCGGCGGGGTTATTCATAACCGTTTGCTGCGTGCACGTCTGGCGCATTATCTCGCTGATTTCACATTGCTCTTTCCA
CAGAGTTTACCGGCGGGTGATGGCGGTTTGTCTCTGGGGCAGGGGGTTATTGCTGCGGCGCGTTGGTTAGCGGGTGAAGT
CCAGAACGGATAA

Upstream 100 bases:

>100_bases
AATACGGAGCTGGCGGTATCGCCGACCTGAGACTGCCGGATACGACGCAGTCGTGTCCGGCAGGGCGGGTTACGCAATTC
ATGATGGCGGGAATAACTCA

Downstream 100 bases:

>100_bases
AACGTGTTGCATTTGGACACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACACATGCGTCCCCGTAGGTCGGA
TAAGACGCGCCAGCGTCGCA

Product: carbamoyltransferase HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 750; Mature: 749

Protein sequence:

>750_residues
MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFI
WSQLPTEFTIRQSAGGAMNTQIVPDAATCHACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSNAVATLR
ARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVM
TSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPLGFKNVPPVLCLGAD
LKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHA
AACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPE
WQNYSETASVQQQNWSVLARAIERGINAPLASSCGRFFDAVAAALGCAPATLSYEGEAACALEALAASCHGVTHPVTMPR
VDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFP
QSLPAGDGGLSLGQGVIAAARWLAGEVQNG

Sequences:

>Translated_750_residues
MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFI
WSQLPTEFTIRQSAGGAMNTQIVPDAATCHACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSNAVATLR
ARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVM
TSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPLGFKNVPPVLCLGAD
LKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHA
AACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPE
WQNYSETASVQQQNWSVLARAIERGINAPLASSCGRFFDAVAAALGCAPATLSYEGEAACALEALAASCHGVTHPVTMPR
VDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFP
QSLPAGDGGLSLGQGVIAAARWLAGEVQNG
>Mature_749_residues
AKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIW
SQLPTEFTIRQSAGGAMNTQIVPDAATCHACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPA
CDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSNAVATLRA
RKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMT
SGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPLGFKNVPPVLCLGADL
KNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHAA
ACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEW
QNYSETASVQQQNWSVLARAIERGINAPLASSCGRFFDAVAAALGCAPATLSYEGEAACALEALAASCHGVTHPVTMPRV
DNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQ
SLPAGDGGLSLGQGVIAAARWLAGEVQNG

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=750, Percent_Identity=98.6666666666667, Blast_Score=1519, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 81935; Mature: 81804

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEDPETFLVQ
CCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHH
LHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQIVPDAATCHACLAEMNTPG
HHHHCCCHHHHCCCCCCCEEHHCCCCCEEEECCCCCCCCCEECCCHHHHHHHHHHCCCCC
ERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVA
CCEEECCEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCCCCHHHCCHHHHHHCCCCCC
CPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSNAVATLR
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCEEEEE
ARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPGLNEVGVM
HHCCCCCCCEEEEEEHHCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCHHHCCEE
LPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMD
CCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCEEEECHHHHHHHH
DSVVRESGEMLRRSRGYVPDALALPLGFKNVPPVLCLGADLKNTFCLVRGEQAVLSQHLG
HHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCEEEEECCHHHHHHHCC
DLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHH
AACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALPGGD
HHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCEEEECHHHHHHCCCCCEEEECCCC
LAAKQPWRNLLAQCLRFVPEWQNYSETASVQQQNWSVLARAIERGINAPLASSCGRFFDA
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAAALGCAPATLSYEGEAACALEALAASCHGVTHPVTMPRVDNQLDLATFWQQWLNWQAP
HHHHHCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
VNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
QSLPAGDGGLSLGQGVIAAARWLAGEVQNG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEDPETFLVQ
CCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHH
LHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQIVPDAATCHACLAEMNTPG
HHHHCCCHHHHCCCCCCCEEHHCCCCCEEEECCCCCCCCCEECCCHHHHHHHHHHCCCCC
ERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVA
CCEEECCEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCCCCHHHCCHHHHHHCCCCCC
CPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGFHLACDARNSNAVATLR
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCEEEEE
ARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPGLNEVGVM
HHCCCCCCCEEEEEEHHCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCHHHCCEE
LPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMD
CCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCEEEECHHHHHHHH
DSVVRESGEMLRRSRGYVPDALALPLGFKNVPPVLCLGADLKNTFCLVRGEQAVLSQHLG
HHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCEEEEECCHHHHHHHCC
DLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAREMNLPTQTVLHHHAHA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHH
AACLAEHQWPLDGGDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALPGGD
HHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCEEEECHHHHHHCCCCCEEEECCCC
LAAKQPWRNLLAQCLRFVPEWQNYSETASVQQQNWSVLARAIERGINAPLASSCGRFFDA
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VAAALGCAPATLSYEGEAACALEALAASCHGVTHPVTMPRVDNQLDLATFWQQWLNWQAP
HHHHHCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
VNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
QSLPAGDGGLSLGQGVIAAARWLAGEVQNG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8661925; 12206761 [H]