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The map label for this gene is proW

Identifier: 157162129

GI number: 157162129

Start: 2823453

End: 2824517

Strand: Direct

Name: proW

Synonym: EcHS_A2814

Alternate gene names: 157162129

Gene position: 2823453-2824517 (Clockwise)

Preceding gene: 157162128

Following gene: 157162130

Centisome position: 60.8

GC content: 58.12

Gene sequence:

>1065_bases
ATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGCGCAATCCGCAGACGCCTGGGGTACACC
GACGACTGCACCGACTGACGGCGGTGGTGCTGACTGGCTGACCAGTACGCCTGCGCCAAACGTCGAGCATTTTAATATTC
TCGATCCGTTCCATAAAACGCTGATCCCGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGT
CCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATTGCTGCTGGGTATGCCCGCACC
GGTGGCGATTATCGTTTTCGCTCTCATCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGA
TTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATC
GTCATCGGTTTGCCGTTGGGGATATGGCTGGCGAGAAGTCCGCGAGCGGCGAAAATTATTCGTCCACTGCTTGATGCCAT
GCAGACCACGCCAGCGTTTGTTTATCTGGTGCCAATCGTCATGCTATTTGGTATCGGTAACGTGCCGGGCGTGGTGGTGA
CGATCATCTTTGCTCTGCCGCCGATTATCCGTCTGACCATTCTGGGGATTAACCAGGTTCCGGCGGATCTGATTGAAGCC
TCGCGCTCATTCGGTGCCAGCCCGCGCCAGATGCTGTTCAAAGTTCAGTTACCGCTGGCGATGCCGACCATTATGGCGGG
CGTTAACCAGACGCTGATGCTGGCCCTTTCTATGGTGGTCATCGCCTCGATGATTGCCGTCGGCGGGTTGGGTCAGATGG
TACTTCGCGGTATCGGTCGTCTGGATATGGGGCTTGCCACCGTTGGCGGCGTCGGGATTGTGATCCTCGCCATTATCCTC
GATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCT
GCTGACCCGCCCATTCATTAAGTAA

Upstream 100 bases:

>100_bases
GCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTT
AGATCGTGAGGGGGTAAATA

Downstream 100 bases:

>100_bases
CTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGC
GACAGCGTTTGCCACGCTTA

Product: glycine betaine transporter membrane protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTPAPNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFR
PVFQGVRVPVDYILNGFQQLLLGMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIIRLTILGINQVPADLIEA
SRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIIL
DRLTQAVGRDSRSRGNRRWYTTGPVGLLTRPFIK

Sequences:

>Translated_354_residues
MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTPAPNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFR
PVFQGVRVPVDYILNGFQQLLLGMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIIRLTILGINQVPADLIEA
SRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIIL
DRLTQAVGRDSRSRGNRRWYTTGPVGLLTRPFIK
>Mature_353_residues
ADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTPAPNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRP
VFQGVRVPVDYILNGFQQLLLGMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCIV
IGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIIRLTILGINQVPADLIEAS
RSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILD
RLTQAVGRDSRSRGNRRWYTTGPVGLLTRPFIK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1789033, Length=354, Percent_Identity=100, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=32.4607329842932, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788449, Length=184, Percent_Identity=29.8913043478261, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786564, Length=154, Percent_Identity=31.8181818181818, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROW_ECOLI (P14176)

Other databases:

- EMBL:   M24856
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   JS0129
- RefSeq:   AP_003251.1
- RefSeq:   NP_417164.1
- ProteinModelPortal:   P14176
- SMR:   P14176
- STRING:   P14176
- EnsemblBacteria:   EBESCT00000003580
- EnsemblBacteria:   EBESCT00000003581
- EnsemblBacteria:   EBESCT00000003582
- EnsemblBacteria:   EBESCT00000017201
- GeneID:   947145
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2653
- KEGG:   eco:b2678
- EchoBASE:   EB0765
- EcoGene:   EG10772
- eggNOG:   COG4176
- GeneTree:   EBGT00050000008565
- HOGENOM:   HBG592565
- OMA:   FNIMDPF
- ProtClustDB:   PRK10952
- BioCyc:   EcoCyc:PROW-MONOMER
- Genevestigator:   P14176
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 37620; Mature: 37489

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1627d9f4)-; HASH(0x16b72d48)-; HASH(0x16babc78)-; HASH(0x16bd57c0)-; HASH(0x169e6b84)-; HASH(0x16ca5c68)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTPAPNVEHFNILDPFHKT
CCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLLGMPAPVAIIVFALIAWQI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCIVIGLPLGIWLARSPRAAKII
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIIRLTILGINQVPADLIEA
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGR
HHHCCCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCH
LDMGLATVGGVGIVILAIILDRLTQAVGRDSRSRGNRRWYTTGPVGLLTRPFIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEECCCHHHHCCCCCH
>Mature Secondary Structure 
ADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTPAPNVEHFNILDPFHKT
CCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLLGMPAPVAIIVFALIAWQI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCIVIGLPLGIWLARSPRAAKII
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFALPPIIRLTILGINQVPADLIEA
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGR
HHHCCCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCH
LDMGLATVGGVGIVILAIILDRLTQAVGRDSRSRGNRRWYTTGPVGLLTRPFIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEECCCHHHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503