Click here to switch to the map view.

The map label for this gene is pheA

Identifier: 157162073

GI number: 157162073

Start: 2777358

End: 2778518

Strand: Direct

Name: pheA

Synonym: EcHS_A2756

Alternate gene names: 157162073

Gene position: 2777358-2778518 (Clockwise)

Preceding gene: 157162072

Following gene: 157162076

Centisome position: 59.81

GC content: 49.01

Gene sequence:

>1161_bases
ATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCATTACTCGCAGA
GCGGCGCGAACTGGCCGTCGAGGTGGGAAAAGCCAAACTGCTCTCGCATCGCCCGGTACGTGATATTGATCGTGAACGCG
ATTTACTGGAAAGATTAATTACGCTCGGTAAAGCGCACCATCTGGACGCCCATTACATTACTCGCCTGTTCCAGCTCATC
ATTGAAGATTCCGTATTAACTCAGCAGGCTTTGCTCCAACAACATCTCAATAAAATTAATCCGCACTCAGCACGCATCGC
TTTTCTCGGCCCCAAAGGCTCCTATTCACATCTTGCCGCTCGTCAGTACGCTGCCCGTCACTTTGAGCAATTCATTGAAA
GTGGCTGCGCCAAATTTGCCGATATTTTTAATCAGGTGGAAACCGGCCAGGCCGACTATGCCGTCGTACCGATTGAAAAT
ACCAGCTCCGGTGCCATAAACGACGTTTACGATCTGCTGCAACATACCAGCTTGTCGATTGTTGGCGAGATGACGTTAAC
TATCGACCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATCAATACGGTCTACAGCCATCCGCAGCCATTCC
AGCAATGCAGCAAATTCCTTAATCGTTATCCGCACTGGAAGATTGAATATACCGAAAGTACGTCTGCGGCAATGGAAAAG
GTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGA
GCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGG
TTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAAC
CACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCA
GGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATTACCCGTTCAATGAAGGTATTGGGCT
GTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGA

Upstream 100 bases:

>100_bases
TGAAAGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGCCTTTTTTATT
GATAACAAAAAGGCAACACT

Downstream 100 bases:

>100_bases
TGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGGTTGTCATTCGCCTGACGCAATAACACGC
GGCTTTCACTCTGAAAACGC

Product: bifunctional chorismate mutase/prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLI
IEDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEK
VAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQNFTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRN
HNLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT

Sequences:

>Translated_386_residues
MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLI
IEDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEK
VAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQNFTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRN
HNLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT
>Mature_385_residues
TSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLII
EDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIENT
SSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEKV
AQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQNFTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNH
NLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain

Homologues:

Organism=Escherichia coli, GI1788951, Length=386, Percent_Identity=100, Blast_Score=799, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324013, Length=323, Percent_Identity=26.625386996904, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHEA_ECO57 (P0A9J9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F91061
- RefSeq:   NP_289152.1
- RefSeq:   NP_311489.1
- ProteinModelPortal:   P0A9J9
- EnsemblBacteria:   EBESCT00000026101
- EnsemblBacteria:   EBESCT00000056625
- GeneID:   914858
- GeneID:   958015
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3891
- KEGG:   ecs:ECs3462
- GeneTree:   EBGT00050000010437
- HOGENOM:   HBG693866
- OMA:   LESRPIY
- ProtClustDB:   PRK10622
- BioCyc:   ECOL83334:ECS3462-MONOMER
- GO:   GO:0005737
- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010952
- InterPro:   IPR001086
- InterPro:   IPR018528
- Gene3D:   G3DSA:1.20.59.10
- PIRSF:   PIRSF001500
- SMART:   SM00830
- TIGRFAMs:   TIGR01797

Pfam domain/function: PF01817 CM_2; PF00800 PDT; SSF48600 Chorismate_mut

EC number: =5.4.99.5; =4.2.1.51

Molecular weight: Translated: 43112; Mature: 42981

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS51168 CHORISMATE_MUT_2; PS00857 PREPHENATE_DEHYDR_1; PS00858 PREPHENATE_DEHYDR_2; PS51171 PREPHENATE_DEHYDR_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAA
HHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
RQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIENTSSGAINDVYDLLQHTSLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHH
VGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEK
HHHHHEEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
VAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQNFTRFVVLARKAINVSDQVPAKTTL
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEE
LMATGQQAGALVEALLVLRNHNLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALK
EEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHEEEEEEECCCCHHHHHHHHH
ELGEITRSMKVLGCYPSENVVPVDPT
HHHHHHHHHEEEEECCCCCEEECCCC
>Mature Secondary Structure 
TSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAA
HHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
RQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIENTSSGAINDVYDLLQHTSLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHH
VGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEK
HHHHHEEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
VAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQNFTRFVVLARKAINVSDQVPAKTTL
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEE
LMATGQQAGALVEALLVLRNHNLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALK
EEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHEEEEEEECCCCHHHHHHHHH
ELGEITRSMKVLGCYPSENVVPVDPT
HHHHHHHHHEEEEECCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796