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The map label for this gene is mltF

Identifier: 157162035

GI number: 157162035

Start: 2734136

End: 2735692

Strand: Direct

Name: mltF

Synonym: EcHS_A2711

Alternate gene names: 157162035

Gene position: 2734136-2735692 (Clockwise)

Preceding gene: 157162029

Following gene: 157162038

Centisome position: 58.88

GC content: 50.48

Gene sequence:

>1557_bases
TTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCC
CTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCC
TGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTA
AAACTGAAAGTGACCGTGCGGCAGAATATCAGCCAGCTGTTTGACGACCTCGATAATGGTAACGCCGACCTGCTGGCGGC
AGGACTGGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGGCCCTACCTATTATTCCGTGTCACAACAACTGGTTT
ATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTG
GTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGA
ATTAATGGAAGATGTCATCGAAGGAAAACTCGATTACACCATTGCTGATTCTGTCGCCATCAGCCTGTTTCAGCGCGTTC
ACCCGGAGCTCGCCGTAGCGCTCGATATCACCGATGAACAACCGGTGACTTGGTTTAGCCCGTTAGATGGCGATAATACC
CTTTCCGCCGCCCTGCTCGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGG
GCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCC
TGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAG
GCCACTTCACCGACGGGTGTGCGCGGCATGATGATGTTAACCAAAAATACCGCGCAAAGCCTCGGCATTACGGATCGTAC
CGATGCCGAACAGAGCATTAGCGGTGGCGTGCGTTATTTGCAGGATATGATGAGTAAAGTGCCGGAAAGTGTGCCGGAGA
ACGAACGGATCTGGTTTGCCCTCGCTGCGTACAATATGGGCTATGCGCATATGCTGGATGCCCGCGCCCTGACGGCAAAA
ACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCT
GACTTACGGCTACGCTCGTGGGCATGAAGCCTACGCTTATGTCGAAAATATTCGTAAGTATCAGATTAGCCTGGTGGGTT
ATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAG
TTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCT
GTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAA

Upstream 100 bases:

>100_bases
GGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCA
CGACACAGAGAATTAACTAA

Downstream 100 bases:

>100_bases
TCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCA
CTCATCCGCCAGTATTCCTT

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGV
KLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFSPLDGDNT
LSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQ
ATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAK
TKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAAMQLAQDYPAVSPTE
LGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN

Sequences:

>Translated_518_residues
MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGV
KLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFSPLDGDNT
LSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQ
ATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAK
TKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAAMQLAQDYPAVSPTE
LGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN
>Mature_518_residues
MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGV
KLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFSPLDGDNT
LSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQ
ATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAK
TKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAAMQLAQDYPAVSPTE
LGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI171474010, Length=518, Percent_Identity=100, Blast_Score=1068, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLTF_ECO11 (B3Y154)

Other databases:

- EMBL:   AB426059
- ProteinModelPortal:   B3Y154
- HAMAP:   MF_02016
- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT

EC number: NA

Molecular weight: Translated: 58303; Mature: 58303

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: ACT_SITE 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEIN
CCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEEEECCEEEECCC
GKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQ
CCCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCHHEEEEHHEECHHHHCCCC
PGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKFPELSWKVD
CCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHEEECCCHHHHHHHHHHHHCCCCCCEEEEC
DKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFSPLDGDNT
CCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCCCCH
LSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQLKPLFEKYAE
HHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHH
QDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPDSWADVKQRLPLLSQ
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHEECCCCCCCHHHHHHHHCCCCCC
KPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAAMQLAQDYPAVSPTE
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN
CCCCCCHHHHHHHCCCCCCEECCCCCEEECCCCCCCCC
>Mature Secondary Structure
MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVSTIHTPLTYNEIN
CCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEEEECCEEEECCC
GKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAAGLVYNSERVKNYQ
CCCCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCHHEEEEHHEECHHHHCCCC
PGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQTLKETKFPELSWKVD
CCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHEEECCCHHHHHHHHHHHHCCCCCCEEEEC
DKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDITDEQPVTWFSPLDGDNT
CCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCCCCH
LSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFLRAVDAVLPQLKPLFEKYAE
HHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQSLGITDRTDAEQSISGGVRYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHH
QDMMSKVPESVPENERIWFALAAYNMGYAHMLDARALTAKTKGNPDSWADVKQRLPLLSQ
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHEECCCCCCCHHHHHHHHCCCCCC
KPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYLQEKEKQATEAAMQLAQDYPAVSPTE
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LGKEKFPFLSFLSQSSSNYLTHSPSLLFSRKGSEEKQN
CCCCCCHHHHHHHCCCCCCEECCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA