Click here to switch to the map view.

The map label for this gene is hyfR [H]

Identifier: 157161951

GI number: 157161951

Start: 2633903

End: 2635849

Strand: Direct

Name: hyfR [H]

Synonym: EcHS_A2625

Alternate gene names: 157161951

Gene position: 2633903-2635849 (Clockwise)

Preceding gene: 157161950

Following gene: 157161952

Centisome position: 56.72

GC content: 52.75

Gene sequence:

>1947_bases
ATGCTCGCGGAGCGGTTAGCGCAGAAACACGGTAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAG
CCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTATTACCGCTACCATGATGATGGCAGCGATGTGACGG
CAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTA
CGCTTTATTCGCACCCACGGCAGTCGTTTTACTCCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCC
GGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGG
TACTGGTTGATATTACTAATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCAT
CATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTTAGCCTGTGGTGCAGCGA
TCTTTCTGCCTCACATTGTGCGTGTCTGCCACGCAATATGCCTGGCGACAGTGTATTGCTGACACAAACGCTACAAACCC
GACAACCGACCTTGACGCACCGTGCAGACGATCTGTTTCTCTGGCAACGCGACCCGTTATTACTCTTACTTGCATCTAAC
GGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTC
CACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATG
CCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTG
GGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTACTCCAGCAGTTAGATATTGTGGCGAAGAGCGACAG
TACGGTGTTGATTTGCGGTGAAACCGGAACCGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCG
ACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGG
GCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGG
CGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAACGGGAGATTGAGCGTCTCGGCGGGAGTAGAA
CGATCCCGGTAAATGTCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGC
GATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTGCGCGACCGTCCGGAAGATATCCCTCTTTTAGC
AAAGCATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGA
TGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGT
CTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGC
GCAGTTGCTGCACCCGACGACGCCAGAGAATGACGAAGAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATG
GCATTGTTGCCGGGCCCCGTGGCGCGGCGACACGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGTCTG
GGGATCTCGGTTCGCGAGGTGTTGTAA

Upstream 100 bases:

>100_bases
CGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCGCAAGGAATAA
CAATTGAAGCGGTAAACGGA

Downstream 100 bases:

>100_bases
TCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGACTTGCAGTTGAGCGCCAGAAAAGCGGCAATCGCTGA
ACGGATTGCCGCCCATAAAA

Product: formate hydrogenlyase transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MLAERLAQKHGKASLLRAFIPLPPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDITNAVLSHLDLDDLIADVAREIH
HFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACLPRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASN
GCESALLIPLTFGNHTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQLSEQLLSNL
GIGDIIYQSQAMEDLLQQLDIVAKSDSTVLICGETGTGKEVIARAIHQLSPRRDKPLVKINCAAIPASLLESELFGHDKG
AFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRS
DLFYRLNVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRELENVIERAVLLTRGNS
LNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRL
GISVREVL

Sequences:

>Translated_648_residues
MLAERLAQKHGKASLLRAFIPLPPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDITNAVLSHLDLDDLIADVAREIH
HFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACLPRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASN
GCESALLIPLTFGNHTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQLSEQLLSNL
GIGDIIYQSQAMEDLLQQLDIVAKSDSTVLICGETGTGKEVIARAIHQLSPRRDKPLVKINCAAIPASLLESELFGHDKG
AFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRS
DLFYRLNVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRELENVIERAVLLTRGNS
LNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRL
GISVREVL
>Mature_648_residues
MLAERLAQKHGKASLLRAFIPLPPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDITNAVLSHLDLDDLIADVAREIH
HFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACLPRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASN
GCESALLIPLTFGNHTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQLSEQLLSNL
GIGDIIYQSQAMEDLLQQLDIVAKSDSTVLICGETGTGKEVIARAIHQLSPRRDKPLVKINCAAIPASLLESELFGHDKG
AFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRS
DLFYRLNVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRELENVIERAVLLTRGNS
LNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRL
GISVREVL

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=648, Percent_Identity=99.3827160493827, Blast_Score=1315, Evalue=0.0,
Organism=Escherichia coli, GI1789087, Length=573, Percent_Identity=50.6108202443281, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1788550, Length=353, Percent_Identity=48.1586402266289, Blast_Score=292, Evalue=5e-80,
Organism=Escherichia coli, GI1790437, Length=315, Percent_Identity=48.2539682539683, Blast_Score=285, Evalue=8e-78,
Organism=Escherichia coli, GI1788905, Length=232, Percent_Identity=55.1724137931034, Blast_Score=263, Evalue=3e-71,
Organism=Escherichia coli, GI1790299, Length=364, Percent_Identity=42.3076923076923, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=54.8936170212766, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1789233, Length=328, Percent_Identity=42.3780487804878, Blast_Score=239, Evalue=5e-64,
Organism=Escherichia coli, GI1786524, Length=355, Percent_Identity=42.2535211267606, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=48.9177489177489, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1787583, Length=323, Percent_Identity=36.5325077399381, Blast_Score=189, Evalue=7e-49,
Organism=Escherichia coli, GI87081858, Length=306, Percent_Identity=35.2941176470588, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1789828, Length=237, Percent_Identity=37.5527426160338, Blast_Score=142, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 73065; Mature: 73065

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAERLAQKHGKASLLRAFIPLPPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQG
CCHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHEEECCCCEEEECCCCCCEEEEECCCC
EMVDYSRHAVLLGSSGMAELRFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQV
CEECCCCCEEEEECCCCHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
ALRLLEKDRDHHRVLVDITNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEK
HHHHHHCCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FSLWCSDLSASHCACLPRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASN
EEEEECCCCCCHHEECCCCCCCCCEEEEHHHHCCCCCHHCCCCCEEEEECCCEEEEEECC
GCESALLIPLTFGNHTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTD
CCCCEEEEEEECCCCCCCEEEEEECCCHHCCCCHHHHHHHHHHHHEEEECCCHHHHHHHH
LQESLQQENHQLSEQLLSNLGIGDIIYQSQAMEDLLQQLDIVAKSDSTVLICGETGTGKE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCHH
VIARAIHQLSPRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGT
HHHHHHHHCCCCCCCCEEEEEEHHCCHHHHHHHHHCCCCCCEEECCCCCCCEEEECCCCE
LFLDEIGDLPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRS
EEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHH
DLFYRLNVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPG
HHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
NVRELENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEE
CHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHH
ERQRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL
HHHHHHHHHHHCCCEEECCCCHHHHCCHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MLAERLAQKHGKASLLRAFIPLPPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQG
CCHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHEEECCCCEEEECCCCCCEEEEECCCC
EMVDYSRHAVLLGSSGMAELRFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQV
CEECCCCCEEEEECCCCHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
ALRLLEKDRDHHRVLVDITNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEK
HHHHHHCCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FSLWCSDLSASHCACLPRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASN
EEEEECCCCCCHHEECCCCCCCCCEEEEHHHHCCCCCHHCCCCCEEEEECCCEEEEEECC
GCESALLIPLTFGNHTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTD
CCCCEEEEEEECCCCCCCEEEEEECCCHHCCCCHHHHHHHHHHHHEEEECCCHHHHHHHH
LQESLQQENHQLSEQLLSNLGIGDIIYQSQAMEDLLQQLDIVAKSDSTVLICGETGTGKE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCHH
VIARAIHQLSPRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGT
HHHHHHHHCCCCCCCCEEEEEEHHCCHHHHHHHHHCCCCCCEEECCCCCCCEEEECCCCE
LFLDEIGDLPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRS
EEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHH
DLFYRLNVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPG
HHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
NVRELENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEE
CHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHH
ERQRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL
HHHHHHHHHHHCCCEEECCCCHHHHCCHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]