Click here to switch to the map view.

The map label for this gene is narQ

Identifier: 157161928

GI number: 157161928

Start: 2607638

End: 2609338

Strand: Direct

Name: narQ

Synonym: EcHS_A2598

Alternate gene names: 157161928

Gene position: 2607638-2609338 (Clockwise)

Preceding gene: 157161924

Following gene: 157161929

Centisome position: 56.16

GC content: 51.85

Gene sequence:

>1701_bases
GTGATTGTTAAACGACCCGTCTCGGCCAGTCTGGCCCGGGCCTTTTTTTACATTGTGCTGCTGTCGATTCTTTCCACGGG
TATCGCTCTGCTAACTCTGGCGAGCAGTTTGCGCGACGCTGAGGCTATCAATATTGCCGGATCGCTGCGTATGCAGAGTT
ACCGCCTGGGCTACGACTTGCAAAGTGGCAGTCCACAACTCAATGCACATCGCCAGTTATTTCAGCAGGCACTGCATTCA
CCGGTATTAACCAACCTCAACGTCTGGTATGTGCCAGAAGCAGTAAAAACTCGCTATGCGCATCTGAATGCCAACTGGCT
GGAGATGAATAATCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACC
TGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCGGCATCGGT
ATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAG
TCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGAATGAGCTTGGTCTGCTTGCAAAAA
CCTTTAACCAGATGTCGAGCGAGCTGCATAAATTGTACCGTTCGCTGGAAGCGTCAGTAGAAGAAAAGACCCGCGATCTC
CACGAGGCCAAGCGTCGTCTGGAGGTGTTGTATCAGTGTTCGCAGGCGCTGAACACTAGCCAGATTGATGTGCATTGTTT
CCGCCATATTTTGCAGATTGTTCGCGACAATGAAGCGGCTGAATATCTGGAGTTAAATGTCGGTGAAAACTGGCGGATTA
GCGAAGGGCAACCAAACCCGGAATTGCCGATGCAGATTTTACCGGTGACAATGCAAGAGACGGTTTACGGCGAACTGCAC
TGGCAAAATAGTCACGTTTCATCATCAGAACCGCTGCTTAACAGCGTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAA
TCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGG
CTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGT
ATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCT
GCAGCAGGCGGATCTCCCCTCCGCGTTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCG
ACTGCCGTCTGCCAACCCTGGCGCTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAAT
GCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCG
TGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAAC
GGCTGGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCTTTCGCTCTGCGGAGGGT
GAGGAAAGTCAGTTGATGTAA

Upstream 100 bases:

>100_bases
TATTTCGCTATAAACTGTGGCAGATCAAATAATCCCATCACTGACTAAATTGCGTTTCAGCGAACTGGAACATTAATGAT
TTTTTGTGGAGAAGACGCGT

Downstream 100 bases:

>100_bases
AGCCTCCTACTGACCAAAGAATACTTGCACTTAAGGTTCAGTATAAAAGGGCATGATAATTTACATTAACTCCTTTTTTT
CTCCACGATTGGCTCGTACC

Product: nitrate/nitrite sensor protein NarQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAINIAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHS
PVLTNLNVWYVPEAVKTRYAHLNANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTNLPNELGLLAKTFNQMSSELHKLYRSLEASVEEKTRDL
HEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRDNEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELH
WQNSHVSSSEPLLNSVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIPEDNATAQS
IMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAKLTLDCRLPTLALDAQMQVHLLQIIREAVLN
AMKHANASEIAVSCVTAPDGNHTVYIRDNGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEG
EESQLM

Sequences:

>Translated_566_residues
MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAINIAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHS
PVLTNLNVWYVPEAVKTRYAHLNANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTNLPNELGLLAKTFNQMSSELHKLYRSLEASVEEKTRDL
HEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRDNEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELH
WQNSHVSSSEPLLNSVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIPEDNATAQS
IMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAKLTLDCRLPTLALDAQMQVHLLQIIREAVLN
AMKHANASEIAVSCVTAPDGNHTVYIRDNGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEG
EESQLM
>Mature_566_residues
MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAINIAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHS
PVLTNLNVWYVPEAVKTRYAHLNANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTNLPNELGLLAKTFNQMSSELHKLYRSLEASVEEKTRDL
HEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRDNEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELH
WQNSHVSSSEPLLNSVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIPEDNATAQS
IMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAKLTLDCRLPTLALDAQMQVHLLQIIREAVLN
AMKHANASEIAVSCVTAPDGNHTVYIRDNGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEG
EESQLM

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate/nitrite availability to the narL/narP proteins. NarQ probably activates narL and narP by phosphorylation. NarQ probably negatively regulates the narL protein by dephosphorylation

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1788812, Length=566, Percent_Identity=100, Blast_Score=1162, Evalue=0.0,
Organism=Escherichia coli, GI1787474, Length=588, Percent_Identity=29.421768707483, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI87082311, Length=209, Percent_Identity=33.0143540669856, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NARQ_ECOLI (P27896)

Other databases:

- EMBL:   M94724
- EMBL:   X65714
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   U12598
- PIR:   D65022
- RefSeq:   AP_003054.1
- RefSeq:   NP_416964.1
- ProteinModelPortal:   P27896
- DIP:   DIP-10318N
- MINT:   MINT-1307707
- STRING:   P27896
- EnsemblBacteria:   EBESCT00000000889
- EnsemblBacteria:   EBESCT00000015385
- GeneID:   946948
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2453
- KEGG:   eco:b2469
- EchoBASE:   EB1429
- EcoGene:   EG11460
- eggNOG:   COG3850
- GeneTree:   EBGT00050000008667
- HOGENOM:   HBG758462
- OMA:   ISNILAR
- ProtClustDB:   PRK10935
- BioCyc:   EcoCyc:NARQ-MONOMER
- BRENDA:   2.7.13.3
- Genevestigator:   P27896
- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR011712
- InterPro:   IPR005467
- Gene3D:   G3DSA:3.30.565.10
- PIRSF:   PIRSF003167
- SMART:   SM00304
- SMART:   SM00387

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3; SSF55874 ATP_bd_ATPase

EC number: =2.7.13.3

Molecular weight: Translated: 63698; Mature: 63698

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS50885 HAMP; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x165c7754)-; HASH(0x1652c7c0)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAINIAGSLRMQSYRLGYDL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHEECCEEC
QSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHLNANWLEMNNRLSKGDLPW
CCCCCCHHHHHHHHHHHHCCCHHCCCEEEECCHHHHHHHHHCCCHHHHHCCCCCCCCCCC
YQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIGIFTLVFFTLRRIRHQVVAPL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
NQLVTASQRIEHGQFDSPPLDTNLPNELGLLAKTFNQMSSELHKLYRSLEASVEEKTRDL
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRDNEAAEYLELNVGENWRISEGQPNP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHEEEEECCCCEEECCCCCCC
ELPMQILPVTMQETVYGELHWQNSHVSSSEPLLNSVSSMLGRGLYFNQAQKHFQQLLLME
CCCCEEECCHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ERATIARELHDSLAQVLSYLRIQLTLLKRSIPEDNATAQSIMADFSQALNDAYRQLRELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TTFRLTLQQADLPSALREMLDTLQNQTSAKLTLDCRLPTLALDAQMQVHLLQIIREAVLN
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHH
AMKHANASEIAVSCVTAPDGNHTVYIRDNGIGIGEPKEPEGHYGLNIMRERAERLGGTLT
HHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEE
FSQPSGGGTLVSISFRSAEGEESQLM
ECCCCCCCEEEEEEEECCCCCCCCCH
>Mature Secondary Structure
MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAINIAGSLRMQSYRLGYDL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHEECCEEC
QSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHLNANWLEMNNRLSKGDLPW
CCCCCCHHHHHHHHHHHHCCCHHCCCEEEECCHHHHHHHHHCCCHHHHHCCCCCCCCCCC
YQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIGIFTLVFFTLRRIRHQVVAPL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
NQLVTASQRIEHGQFDSPPLDTNLPNELGLLAKTFNQMSSELHKLYRSLEASVEEKTRDL
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRDNEAAEYLELNVGENWRISEGQPNP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHEEEEECCCCEEECCCCCCC
ELPMQILPVTMQETVYGELHWQNSHVSSSEPLLNSVSSMLGRGLYFNQAQKHFQQLLLME
CCCCEEECCHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ERATIARELHDSLAQVLSYLRIQLTLLKRSIPEDNATAQSIMADFSQALNDAYRQLRELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TTFRLTLQQADLPSALREMLDTLQNQTSAKLTLDCRLPTLALDAQMQVHLLQIIREAVLN
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHH
AMKHANASEIAVSCVTAPDGNHTVYIRDNGIGIGEPKEPEGHYGLNIMRERAERLGGTLT
HHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEE
FSQPSGGGTLVSISFRSAEGEESQLM
ECCCCCCCEEEEEEEECCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1528845; 1508040; 9205837; 9278503