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The map label for this gene is psuG [H]

Identifier: 157161646

GI number: 157161646

Start: 2299606

End: 2300544

Strand: Reverse

Name: psuG [H]

Synonym: EcHS_A2302

Alternate gene names: 157161646

Gene position: 2300544-2299606 (Counterclockwise)

Preceding gene: 157161647

Following gene: 157161644

Centisome position: 49.54

GC content: 49.09

Gene sequence:

>939_bases
ATGTCTAAATTAAAAATTTCCCCTGAATTATTACAAATTTCCCCGGAAGTGCAGGAAGCTTTAAAAAACAAAAAACCGGT
TGTGGCGCTGGAATCGACCATTATTTCTCACGGGATGCCGTTCCCACAAAATGCCCAGACCGCAATTGAAGTAGAAGAAA
CTATTCGTAAACAGGGCGCAGTACCTGCCACTATCGCCATTATTGGCGGCGTGATGAAAGTGGGTTTAAGTAAAGAAGAA
ATTGAATTACTGGGTCGTGAAGGGCATAACGTGACCAAAGTTAGTCGTCGCGATTTACCTTTTGTTGTTGCCGCCGGAAA
AAATGGCGCAACCACTGTGGCTTCAACGATGATTATTGCGGCGCTTGCCGGAATTAAAGTATTTGCCACCGGGGGAATTG
GTGGTGTGCATCGCGGGGCGGAACATACCTTCGATATTTCTGCCGATTTGCAAGAACTGGCAAATACTAATGTCACCGTT
GTTTGTGCCGGGGCGAAATCTATTCTCGATTTAGGATTAACCACTGAGTATTTAGAAACCTTCGGTGTGCCGTTAATTGG
CTATCAGACTAAAGCGCTGCCTGCGTTTTTCTGCCGTACCAGCTCGTTTGACGTCAGCATTCGTCTCGACAGCGCCAGTG
AAATTGCCCGTGCAATGGCGGTGAAATGGCAAAGCGGGCTGAACGGTGGCCTCGTGGTAGCGAACCCGATCCCGGAACAG
TTTGCGATGCCGGAGGAATCTATCAATGCAGCCATAGATCAAGCCGTCGCCGAAGCCGAAGAGCAGGGCGTTATTGGTAA
AGAAAGTACACCGTTCCTGCTGGCGCGCGTTGCTGAACTGACCGGCGGTGACAGCCTGAAATCCAATATCCAGCTGGTGT
TCAACAACGCCATTCTGGCGAGTGAAATTGCCAAAGAATACCAGCGTCTCGCGGGTTAA

Upstream 100 bases:

>100_bases
GTTTCGCACAGGGATGTTCGTCAATGGCGCTCTCCTGTGAATACACCAATAACCCCGATTTATCGATTGCCAACGTTATA
TCGTTAGTGGAGAACGCAGA

Downstream 100 bases:

>100_bases
TTTCTGACGGGCAGAAAGCCGTCGCCCGTACTCATCAAACATACCAAAACCTGGCGCTGACGCCGGGTTTACTGGCATGA
AGGGAAAATCATTATGGACA

Product: indigoidine synthase A like protein

Products: NA

Alternate protein names: PsiMP glycosidase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSKLKISPELLQISPEVQEALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEE
IELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQ
FAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG

Sequences:

>Translated_312_residues
MSKLKISPELLQISPEVQEALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEE
IELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQ
FAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG
>Mature_311_residues
SKLKISPELLQISPEVQEALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEI
ELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVV
CAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQF
AMPEESINAAIDQAVAEAEEQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG

Specific function: Catalyzes the hydrolysis of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil. In vitro, can also catalyze the reverse reaction [H]

COG id: COG2313

COG function: function code Q; Uncharacterized enzyme involved in pigment biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine-5'-phosphate glycosidase family [H]

Homologues:

Organism=Escherichia coli, GI1788490, Length=312, Percent_Identity=97.7564102564102, Blast_Score=615, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI25147470, Length=297, Percent_Identity=51.1784511784512, Blast_Score=280, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24580669, Length=295, Percent_Identity=52.2033898305085, Blast_Score=288, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022830
- InterPro:   IPR007342 [H]

Pfam domain/function: PF04227 Indigoidine_A [H]

EC number: NA

Molecular weight: Translated: 32921; Mature: 32790

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLKISPELLQISPEVQEALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGA
CCCCCCCHHHHCCCHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
VPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIA
CCHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHH
ALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTEYLET
HHHCCEEEEECCCCCCCCCCCCEEECCHHHHHHCCCCEEEEECCHHHHHHHCCHHHHHHH
FGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQ
HCCCEECCCHHHCHHHEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHH
FAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILA
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHCCEEEEECCHHHH
SEIAKEYQRLAG
HHHHHHHHHHCC
>Mature Secondary Structure 
SKLKISPELLQISPEVQEALKNKKPVVALESTIISHGMPFPQNAQTAIEVEETIRKQGA
CCCCCCHHHHCCCHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
VPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNGATTVASTMIIA
CCHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHH
ALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSILDLGLTTEYLET
HHHCCEEEEECCCCCCCCCCCCEEECCHHHHHHCCCCEEEEECCHHHHHHHCCHHHHHHH
FGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARAMAVKWQSGLNGGLVVANPIPEQ
HCCCEECCCHHHCHHHEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHH
FAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLARVAELTGGDSLKSNIQLVFNNAILA
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHCCEEEEECCHHHH
SEIAKEYQRLAG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]