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The map label for this gene is amsG [H]

Identifier: 157161544

GI number: 157161544

Start: 2171069

End: 2172496

Strand: Reverse

Name: amsG [H]

Synonym: EcHS_A2190

Alternate gene names: 157161544

Gene position: 2172496-2171069 (Counterclockwise)

Preceding gene: 157161545

Following gene: 157161543

Centisome position: 46.79

GC content: 34.8

Gene sequence:

>1428_bases
ATGAGGCTTCTCGTAAAGAATGTCATAGTTAGTATCACATTGGCTTTATCAGATTTCATTTCATTCATTCTTTCTTTATA
CATAGCGTTGGATGTGTTGTCATTAATTGTAGAAAAGAATGATTATTTATTATTAACTAATGAAATTGATGGGTGGTTTT
TACTTCATTGGATTCTCGGTGTTTGTTGTGTAGGCTGGTATTCAGTTAGACTAAGGCATTATTTTTATCGTAAACCATTT
TGGTTTGAACTTAAAGAAATACTTCGAACCTTGGTTATTTTTGCAATAATCGAAATTGCTGTTATGGCTTTTACCACTTG
GTCATTCTCACGTTACTTATGGGTGATAACGTGGTTATTTGTAATCATACTTGTTCCATTGTCAAGAATGGTAACAAAGA
CCTTATTGAATAAAGCGGGGGTGTGGCAAAGAGATACTTGGATAGTAGGTAGTAGTGAAAATGCTCATGAAGCATATAAA
GCAATTAGCGGAGAAAAAAACTTAGGTTTAAACATTGTCGGTTTCATATCAAGTCCTGGCGGAGTTCCATCAGGAGAGGC
AATTGATGGTATACCAGTGATTGAAAATAATCTAGAATGGCTTAATGATATTGATCCCAAAACACAATTCATTGTTGCTG
TTGAGTCTCATCAAAGTAAAATAAGAAATGCGTGGCTTAGAAACTTTATGATTAAAGGCTATCGATATGTCTCAGTTATT
CCAACATTGCGTGGAATGCCTCTTGATAGCACAGATATGTCATTCATATTTAGTCATGAAGTTATGATTTTTCGTGTACA
GCAAAATCTAGCCAAATGGTCGTCAAGAATTCTCAAAAGACTTTTTGATATTATAGGCTCATTGTCTATAATTACTCTTC
TTTCACCAGTATTATTATATATAAGTCTTAAAGTTAAAAAAGATGGAGGCCCGGCAATATATGGTCATGAAAGGATCGGG
AAAGGAGGTAAACCCTTTAAGTGTTTGAAGTTCAGGTCTATGGTAATCAATTCCAAAGAAGTTCTTGAAGAACTTCTAAA
TAACGATATAAATGCGCGAGAAGAATGGAATTTAACATTCAAATTGAAAAACGACCCAAGAATAACTAAGATAGGCGGTT
TCCTTAGAAGGACAAGCCTGGATGAATTGCCGCAGTTATTTAATGTATTAAAAGGGGAAATGAGTTTAGTTGGACCAAGG
CCTATCATTACTGCTGAGTTAGAAAGATACAATGAAGAAGTTGATTATTATTTATTGAGCAAACCAGGAATGACAGGCCT
ATGGCAAGTTAGTGGGCGTAGTGATGTGGATTATGAGACACGCGTTTATCTTGATGCTTGGTACGTTAAAAATTGGTCAA
TGTGGAATGATATCGCGATTTTATTTAAAACTGTGGGTGTTGTATTAAAAAGAGACGGTGCGTATTAA

Upstream 100 bases:

>100_bases
GAATGCTTGCCCCAATTATCTTTATTGATATTTGGGGTAATGGGAAGGATAATTTATATTAGTGTAACAATATATATTTT
ATTAATTTTGAGAGGATTTC

Downstream 100 bases:

>100_bases
CTTCCAGCCTTTATATAACAGTCAGGATAATCTATCAATGAAATACTATATTGCCATCCCAACTTATAATGGTGGGGATT
TGTGGCGTACTACCGTCGAT

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MRLLVKNVIVSITLALSDFISFILSLYIALDVLSLIVEKNDYLLLTNEIDGWFLLHWILGVCCVGWYSVRLRHYFYRKPF
WFELKEILRTLVIFAIIEIAVMAFTTWSFSRYLWVITWLFVIILVPLSRMVTKTLLNKAGVWQRDTWIVGSSENAHEAYK
AISGEKNLGLNIVGFISSPGGVPSGEAIDGIPVIENNLEWLNDIDPKTQFIVAVESHQSKIRNAWLRNFMIKGYRYVSVI
PTLRGMPLDSTDMSFIFSHEVMIFRVQQNLAKWSSRILKRLFDIIGSLSIITLLSPVLLYISLKVKKDGGPAIYGHERIG
KGGKPFKCLKFRSMVINSKEVLEELLNNDINAREEWNLTFKLKNDPRITKIGGFLRRTSLDELPQLFNVLKGEMSLVGPR
PIITAELERYNEEVDYYLLSKPGMTGLWQVSGRSDVDYETRVYLDAWYVKNWSMWNDIAILFKTVGVVLKRDGAY

Sequences:

>Translated_475_residues
MRLLVKNVIVSITLALSDFISFILSLYIALDVLSLIVEKNDYLLLTNEIDGWFLLHWILGVCCVGWYSVRLRHYFYRKPF
WFELKEILRTLVIFAIIEIAVMAFTTWSFSRYLWVITWLFVIILVPLSRMVTKTLLNKAGVWQRDTWIVGSSENAHEAYK
AISGEKNLGLNIVGFISSPGGVPSGEAIDGIPVIENNLEWLNDIDPKTQFIVAVESHQSKIRNAWLRNFMIKGYRYVSVI
PTLRGMPLDSTDMSFIFSHEVMIFRVQQNLAKWSSRILKRLFDIIGSLSIITLLSPVLLYISLKVKKDGGPAIYGHERIG
KGGKPFKCLKFRSMVINSKEVLEELLNNDINAREEWNLTFKLKNDPRITKIGGFLRRTSLDELPQLFNVLKGEMSLVGPR
PIITAELERYNEEVDYYLLSKPGMTGLWQVSGRSDVDYETRVYLDAWYVKNWSMWNDIAILFKTVGVVLKRDGAY
>Mature_475_residues
MRLLVKNVIVSITLALSDFISFILSLYIALDVLSLIVEKNDYLLLTNEIDGWFLLHWILGVCCVGWYSVRLRHYFYRKPF
WFELKEILRTLVIFAIIEIAVMAFTTWSFSRYLWVITWLFVIILVPLSRMVTKTLLNKAGVWQRDTWIVGSSENAHEAYK
AISGEKNLGLNIVGFISSPGGVPSGEAIDGIPVIENNLEWLNDIDPKTQFIVAVESHQSKIRNAWLRNFMIKGYRYVSVI
PTLRGMPLDSTDMSFIFSHEVMIFRVQQNLAKWSSRILKRLFDIIGSLSIITLLSPVLLYISLKVKKDGGPAIYGHERIG
KGGKPFKCLKFRSMVINSKEVLEELLNNDINAREEWNLTFKLKNDPRITKIGGFLRRTSLDELPQLFNVLKGEMSLVGPR
PIITAELERYNEEVDYYLLSKPGMTGLWQVSGRSDVDYETRVYLDAWYVKNWSMWNDIAILFKTVGVVLKRDGAY

Specific function: Involved in the biosynthesis of amylovoran which functions as a virulence factor. May act as a sugar transferase and may be involved in the export of the repeating unit by flipping the lipid carrier to the periplasmic face of the inner membrane [H]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=350, Percent_Identity=32.5714285714286, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017472 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 54702; Mature: 54702

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLVKNVIVSITLALSDFISFILSLYIALDVLSLIVEKNDYLLLTNEIDGWFLLHWILG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
VCCVGWYSVRLRHYFYRKPFWFELKEILRTLVIFAIIEIAVMAFTTWSFSRYLWVITWLF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIILVPLSRMVTKTLLNKAGVWQRDTWIVGSSENAHEAYKAISGEKNLGLNIVGFISSPG
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCC
GVPSGEAIDGIPVIENNLEWLNDIDPKTQFIVAVESHQSKIRNAWLRNFMIKGYRYVSVI
CCCCCCCCCCCCEECCCCHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCEEEEEEE
PTLRGMPLDSTDMSFIFSHEVMIFRVQQNLAKWSSRILKRLFDIIGSLSIITLLSPVLLY
HHHCCCCCCCCCCCEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISLKVKKDGGPAIYGHERIGKGGKPFKCLKFRSMVINSKEVLEELLNNDINAREEWNLTF
EEEEEECCCCCEEECHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEE
KLKNDPRITKIGGFLRRTSLDELPQLFNVLKGEMSLVGPRPIITAELERYNEEVDYYLLS
EECCCCCEEHHHHHHHHCCHHHHHHHHHHHCCCHHHCCCCCEEHHHHHHCCCCCCEEEEE
KPGMTGLWQVSGRSDVDYETRVYLDAWYVKNWSMWNDIAILFKTVGVVLKRDGAY
CCCCCCEEEECCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRLLVKNVIVSITLALSDFISFILSLYIALDVLSLIVEKNDYLLLTNEIDGWFLLHWILG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
VCCVGWYSVRLRHYFYRKPFWFELKEILRTLVIFAIIEIAVMAFTTWSFSRYLWVITWLF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIILVPLSRMVTKTLLNKAGVWQRDTWIVGSSENAHEAYKAISGEKNLGLNIVGFISSPG
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCC
GVPSGEAIDGIPVIENNLEWLNDIDPKTQFIVAVESHQSKIRNAWLRNFMIKGYRYVSVI
CCCCCCCCCCCCEECCCCHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCEEEEEEE
PTLRGMPLDSTDMSFIFSHEVMIFRVQQNLAKWSSRILKRLFDIIGSLSIITLLSPVLLY
HHHCCCCCCCCCCCEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISLKVKKDGGPAIYGHERIGKGGKPFKCLKFRSMVINSKEVLEELLNNDINAREEWNLTF
EEEEEECCCCCEEECHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEE
KLKNDPRITKIGGFLRRTSLDELPQLFNVLKGEMSLVGPRPIITAELERYNEEVDYYLLS
EECCCCCEEHHHHHHHHCCHHHHHHHHHHHCCCHHHCCCCCEEHHHHHHCCCCCCEEEEE
KPGMTGLWQVSGRSDVDYETRVYLDAWYVKNWSMWNDIAILFKTVGVVLKRDGAY
CCCCCCEEEECCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]