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The map label for this gene is insB1 [H]

Identifier: 157161530

GI number: 157161530

Start: 2156091

End: 2156588

Strand: Reverse

Name: insB1 [H]

Synonym: EcHS_A2176

Alternate gene names: 157161530

Gene position: 2156588-2156091 (Counterclockwise)

Preceding gene: 157161531

Following gene: 157161529

Centisome position: 46.44

GC content: 53.41

Gene sequence:

>498_bases
ATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGT
AACCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAAT
CACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGAAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTG
GCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATGGATGACGGATGGCTGGCCGCTGTATGA
ATCGCGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAGACAACATC
TGGCAAGGCTGGGACGGAAGTCACTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAGGTCATCGGGCATTATCTGAAC
ATAAAACACTATCAGTAA

Upstream 100 bases:

>100_bases
GCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTACGCACCAGAAAATCATTGAT
ATGGCCATGAATGGCGTCGG

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCCCGTTCTACATAAGTGCCGATTTTACACTGGCTTGCGGTTGGGATCACTTGCTTTGCCGCGTCAT
CTCCGCCAGACGTGAGGTGA

Product: IS1, transposase orfB

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLRQHLARLGRKSLSFSKSVELHDKVIGHYLN
IKHYQ

Sequences:

>Translated_165_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLRQHLARLGRKSLSFSKSVELHDKVIGHYLN
IKHYQ
>Mature_164_residues
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLA
TLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNI
KHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=167, Percent_Identity=89.8203592814371, Blast_Score=310, Evalue=3e-86,
Organism=Escherichia coli, GI1788203, Length=167, Percent_Identity=89.8203592814371, Blast_Score=310, Evalue=3e-86,
Organism=Escherichia coli, GI1786203, Length=167, Percent_Identity=89.8203592814371, Blast_Score=310, Evalue=3e-86,
Organism=Escherichia coli, GI1787223, Length=167, Percent_Identity=88.0239520958084, Blast_Score=305, Evalue=1e-84,
Organism=Escherichia coli, GI1786470, Length=167, Percent_Identity=88.0239520958084, Blast_Score=303, Evalue=4e-84,
Organism=Escherichia coli, GI1786459, Length=167, Percent_Identity=88.0239520958084, Blast_Score=303, Evalue=4e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 19510; Mature: 19379

Theoretical pI: Translated: 10.80; Mature: 10.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCHHHHHHHH
YTQRIERHNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
YAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCCHHHHHHHH
YTQRIERHNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]