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The map label for this gene is nac [H]

Identifier: 157161472

GI number: 157161472

Start: 2099619

End: 2100536

Strand: Reverse

Name: nac [H]

Synonym: EcHS_A2111

Alternate gene names: 157161472

Gene position: 2100536-2099619 (Counterclockwise)

Preceding gene: 157161473

Following gene: 157161471

Centisome position: 45.24

GC content: 48.91

Gene sequence:

>918_bases
ATGAACTTCAGACGCCTGAAATACTTCGTAAAAATTGTAGATATTGGTAGCCTGACTCAGGCTGCTGAAGTATTGCATAT
CGCACAACCAGCGCTCAGCCAGCAGGTTGCCACACTGGAAGGTGAGTTAAATCAACAACTTTTGATTCGCACAAAACGGG
GCGTTACGCCAACAGACGCCGGAAAAATTCTCTATACCCATGCGCGGGCCATTTTACGTCAGTGTGAGCAGGCCCAACTG
GCGGTGCATAACGTTGGTCAGGCATTATCGGGGCAAGTCTCGATTGGCTTTGCGCCAGGAACCGCTGCGTCATCTATCAC
CATGCCCTTATTACAAGCGGTTCGCGCTGAATTCCCGGAGATCGTTATCTATCTTCATGAAAATAGCGGTGCAGTGCTTA
ACGAAAAACTGATAAATCACCAACTCGATATGGCGGTGATTTATGAGCATTCCCCTGTGGCTGGGGTATCCAGTCAGGCT
TTGCTGAAAGAAGATCTTTTCCTGGTAGGAACTCAAGATTGCCCGGGGCAAAGCGTTGATGTTAATGCTATTGCGCAAAT
GAACCTCTTTCTCCCCAGTGATTACAGTGCTGTTAGACTTCGTGTTGATGAGGCTTTTTCCCTACGGCGGCTCACGGCAA
AAGTCATTGGTGAAATTGAGTCTATTGCCACGCTTACCGCAGCGATTGCCAGCGGCATGGGCGTTGCAGTATTACCCGAA
TCGGCCGCGCGTTCGTTATGTGGCGCAGTAAATGGGTGGATGTCACGCATTACCACGCCTTCCATGAGTCTCTCTTTGTC
ATTAAATTTACCCGCCAGAGCGAACTTATCGCCACAGGCACAGGCAGTGAAAGAGTTGTTAATGTCAGTGATTAGTTCTC
CAGTGATGGAAAAAAGGCAGTGGCAATTGGTGAGCTAA

Upstream 100 bases:

>100_bases
TAGCTTGTACATCAACGCCAAAATAAAACTGGCAAGCATCTTGCAGTCTGGTTGTAAGTAATGGCGGCACTTGGGCCGAT
TCTCAAAAACCGGAGGCAAC

Downstream 100 bases:

>100_bases
GCGTTATATCGGGATGGAATAAGATGCGGGTTTTTATTATTTGTTATGCCGGGCATTAGACTTTAACAATAACGGGAAAT
CTGAGCTGCCCGGAGTTTAC

Product: nitrogen assimilation transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQL
AVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS

Sequences:

>Translated_305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQL
AVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS
>Mature_305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQL
AVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=305, Percent_Identity=99.672131147541, Blast_Score=619, Evalue=1e-179,
Organism=Escherichia coli, GI157672245, Length=245, Percent_Identity=30.6122448979592, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788748, Length=244, Percent_Identity=28.2786885245902, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=30.0813008130081, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790399, Length=251, Percent_Identity=27.8884462151394, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787879, Length=252, Percent_Identity=28.1746031746032, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788887, Length=175, Percent_Identity=33.1428571428571, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787601, Length=257, Percent_Identity=26.0700389105058, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693105, Length=238, Percent_Identity=23.109243697479, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789204, Length=278, Percent_Identity=24.4604316546763, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI2367136, Length=254, Percent_Identity=25.9842519685039, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786202, Length=248, Percent_Identity=24.5967741935484, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=258, Percent_Identity=24.4186046511628, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=250, Percent_Identity=26.8, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789639, Length=123, Percent_Identity=30.0813008130081, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32821; Mature: 32821

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDA
CCHHHHEHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCC
GKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCE
IVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQALLKEDLFLVGTQDCPGQSVD
EEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCEEEEECCCCCCCCCC
VNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
HHEEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECH
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQ
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCC
WQLVS
CCCCC
>Mature Secondary Structure
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDA
CCHHHHEHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCC
GKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCE
IVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQALLKEDLFLVGTQDCPGQSVD
EEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCEEEEECCCCCCCCCC
VNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
HHEEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECH
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQ
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCC
WQLVS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]