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The map label for this gene is cheR

Identifier: 157161356

GI number: 157161356

Start: 1987675

End: 1988535

Strand: Reverse

Name: cheR

Synonym: EcHS_A1980

Alternate gene names: 157161356

Gene position: 1988535-1987675 (Counterclockwise)

Preceding gene: 157161357

Following gene: 157161355

Centisome position: 42.82

GC content: 54.24

Gene sequence:

>861_bases
ATGACTTCATCTCTGCCCTGTGGGCAAACGTCTTTATTGTTACAGATGACCGAGCGCCTGGCGCTTTCCGACGCGCATTT
TCGGCGGATAAGTCAATTGATCTATCAACGAGCCGGGATCGTTCTGGCTGACCATAAACGCGACATGGTTTACAACCGAC
TGGTTCGTCGTTTGCGTTCGCTGGGACTGACGGATTTCGGTCATTATCTGAACTTGCTGGAATCTAATCAGCACAGCGGT
GAGTGGCAGGCGTTTATCAATTCGCTGACCACGAATCTGACGGCATTTTTCCGTGAGGCACATCATTTCCCTCTGCTCGC
GGATCACGCACGTCGCCGTTCTGGCGAGTATCGCGTATGGAGCGCGGCGGCTTCGACCGGCGAAGAGCCGTACAGCATTG
CGATGACGCTGGCTGACACATTGGGCACCGCGCCCGGACGCTGGAAAGTGTTTGCCAGTGATATCGACACCGAAGTGCTG
GAAAAAGCCAGAAGCGGTATCTATCGCCATGAAGAGTTGAAAAACCTGACGCCGCAGCAACTGCAACGGTATTTCATGCG
AGGGACGGGGCCGCATGAAGGGCTGGTACGCGTGCGTCAGGAGCTGGCGAACTATGTTGATTTTGCCCCGCTGAATCTAC
TGGCGAAACAGTACACCGTGCCGGGGCCGTTTGATGCGATCTTCTGTCGTAACGTCATGATCTACTTCGATCAAACTACC
CAGCAGGAGATTTTGCGCCGCTTTGTTCCGCTCCTTAAACCCGACGGATTGCTGTTTGCGGGTCACTCTGAAAACTTTAG
CCACCTTGAGCGCCGCTTCACGCTGCGTGGTCAGACGGTGTATGCGCTAAGTAAGGATTAA

Upstream 100 bases:

>100_bases
TGTGGCGGTATTTACCCTTGAAGAACATGAAGTAGCACGACATGAGTCGGTGCAGTTACAAATTGCGCCAGTGGTATCCT
GAAGTGATTGAGAAGGCGCT

Downstream 100 bases:

>100_bases
CGATGAGCAAAATCAGGGTGTTATCTGTCGATGATTCGGCACTGATGCGCCAGATCATGACAGAAATCATCAACAGCCAT
AGCGACATGGAAATGGTGGC

Product: chemotaxis methyltransferase CheR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSG
EWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMIYFDQTT
QQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD

Sequences:

>Translated_286_residues
MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSG
EWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMIYFDQTT
QQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD
>Mature_285_residues
TSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGE
WQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLE
KARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMIYFDQTTQ
QEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD

Specific function: Methylation of the membrane-bound methyl-accepting chemotaxis proteins (MCP) to form gamma-glutamyl methyl ester residues in MCP

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain

Homologues:

Organism=Escherichia coli, GI1788193, Length=286, Percent_Identity=100, Blast_Score=593, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHER_ECOLI (P07364)

Other databases:

- EMBL:   M13463
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D64951
- RefSeq:   AP_002504.1
- RefSeq:   NP_416398.1
- ProteinModelPortal:   P07364
- SMR:   P07364
- DIP:   DIP-9273N
- IntAct:   P07364
- STRING:   P07364
- EnsemblBacteria:   EBESCT00000000085
- EnsemblBacteria:   EBESCT00000017142
- GeneID:   946396
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1873
- KEGG:   eco:b1884
- EchoBASE:   EB0146
- EcoGene:   EG10148
- eggNOG:   COG1352
- GeneTree:   EBGT00050000010686
- HOGENOM:   HBG661948
- OMA:   IAGHSEN
- ProtClustDB:   PRK10611
- BioCyc:   EcoCyc:CHER-MONOMER
- Genevestigator:   P07364
- GO:   GO:0006935
- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641
- Gene3D:   G3DSA:1.10.155.10
- PRINTS:   PR00996
- SMART:   SM00138

Pfam domain/function: PF01739 CheR; PF03705 CheR_N; SSF47757 CheR_mtfrase

EC number: =2.1.1.80

Molecular weight: Translated: 32850; Mature: 32719

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50123 CHER

Important sites: BINDING 92-92 BINDING 94-94 BINDING 98-98 BINDING 129-129 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHH
LGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYRVW
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEE
SAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLEKARSGIYRHEELKNLTPQQ
EHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHCCHHHHHHHHHCCHHHHHHHCCCHHH
LQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMIYFDQTT
HHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCCCEEECCCH
QQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
TSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHH
LGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYRVW
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEE
SAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLEKARSGIYRHEELKNLTPQQ
EHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHCCHHHHHHHHHCCHHHHHHHCCCHHH
LQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMIYFDQTT
HHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCCCEEECCCH
QQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3510184; 9097040; 9278503