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The map label for this gene is znuA

Identifier: 157161326

GI number: 157161326

Start: 1960628

End: 1961560

Strand: Reverse

Name: znuA

Synonym: EcHS_A1951

Alternate gene names: 157161326

Gene position: 1961560-1960628 (Counterclockwise)

Preceding gene: 157161329

Following gene: 157161325

Centisome position: 42.24

GC content: 49.09

Gene sequence:

>933_bases
ATGTTACATAAAAAAACGCTTCTTTTCGCAGCATTATCCGCCGCTCTCTGGGGAGGTGCAACACAGGCCGCAGATGCCGC
CGTTGTCGCTTCGCTTAAACCCGTTGGGTTCATCGCTTCTGCCATTGCTGATGGGGTAACAGAAACAGAGGTTTTACTTC
CTGACGGCGCTTCAGAACATGATTATTCACTGCGCCCATCGGATGTAAAACGCTTACAGAACGCGGACTTAGTCGTTTGG
GTTGGCCCGGAGATGGAAGCGTTTATGCAAAAACCGGTAAGCAAATTACCAGGAGCGAAGCAGGTAACGATTGCGCAGCT
TGAAGATGTGAAACCGCTACTGATGAAAAGTATTCACGGCGATGATGATGATCACGACCACGCGGAAAAAAGTGACGAAG
ATCACCATCACGGCGATTTCAACATGCATCTTTGGCTTTCCCCAGAGATAGCGCGGGCTACAGCGGTTGCAATCCATGGA
AAATTAGTGGAACTTATGCCGCAAAGTCGAGCCAAACTTGACGCCAACCTGAAGGATTTTGAGGCACAATTAGCCTCAAC
CGAAACGCAGGTTGGTAACGAGCTCGCGCCGCTCAAGGGGAAAGGTTATTTCGTTTTTCACGATGCTTACGGCTATTTCG
AAAAACAGTTTGGACTGACACCGCTTGGTCATTTTACCGTTAACCCTGAGATTCAACCTGGCGCGCAGCGTTTACATGAA
ATAAGAACACAGTTGGTTGAGCAAAAAGCAACCTGCGTTTTTGCTGAGCCACAGTTCAGGCCAGCGGTCGTTGAGAGCGT
CGCACGAGGGACATCCGTTCGTATGGGAACGTTGGATCCCCTGGGGACGAATATCAAACTGGGTAAAACAAGCTATTCAG
AATTCCTGAGTCAATTAGCCAACCAGTATGCGAGCTGCCTGAAAGGAGATTAA

Upstream 100 bases:

>100_bases
CCAGGGAAACCAGACTTGTCATGTTAATTTTAGTCTTGCAGTAGTCATGAAATGTTATAATATCACACTTCTCATATTCA
TTACGATGATTGGTCGCATT

Downstream 100 bases:

>100_bases
TGAGGAAGTGATTACGTGCAACAGATAGCCCGCTCTGTCGCCCTGGCGTTTAATAATTTACCGCGACCACACCGCGTTAT
GTTGGGGTCGCTCACCGTTC

Product: high-affinity zinc transporter periplasmic component

Products: Zn (II) [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIADGVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVW
VGPEMEAFMQKPVSKLPGAKQVTIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFGLTPLGHFTVNPEIQPGAQRLHE
IRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMGTLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD

Sequences:

>Translated_310_residues
MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIADGVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVW
VGPEMEAFMQKPVSKLPGAKQVTIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFGLTPLGHFTVNPEIQPGAQRLHE
IRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMGTLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD
>Mature_310_residues
MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIADGVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVW
VGPEMEAFMQKPVSKLPGAKQVTIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFGLTPLGHFTVNPEIQPGAQRLHE
IRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMGTLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD

Specific function: Involved in the high-affinity zinc uptake transport system

COG id: COG4531

COG function: function code P; ABC-type Zn2+ transport system, periplasmic component/surface adhesin

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 9 family

Homologues:

Organism=Escherichia coli, GI87081990, Length=310, Percent_Identity=100, Blast_Score=639, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ZNUA_ECOLI (P39172)

Other databases:

- EMBL:   U38702
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A64948
- RefSeq:   AP_002477.1
- RefSeq:   NP_416371.4
- PDB:   2OGW
- PDB:   2OSV
- PDB:   2PRS
- PDB:   2PS0
- PDB:   2PS3
- PDB:   2PS9
- PDBsum:   2OGW
- PDBsum:   2OSV
- PDBsum:   2PRS
- PDBsum:   2PS0
- PDBsum:   2PS3
- PDBsum:   2PS9
- ProteinModelPortal:   P39172
- SMR:   P39172
- DIP:   DIP-12949N
- STRING:   P39172
- SWISS-2DPAGE:   P39172
- 2DBase-Ecoli:   P39172
- EnsemblBacteria:   EBESCT00000003311
- EnsemblBacteria:   EBESCT00000017951
- GeneID:   946375
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5831
- KEGG:   eco:b1857
- EchoBASE:   EB2543
- EcoGene:   EG12678
- eggNOG:   COG4531
- GeneTree:   EBGT00050000011395
- HOGENOM:   HBG731008
- OMA:   KHEHKHD
- ProtClustDB:   PRK09545
- BioCyc:   EcoCyc:ZNUA-MONOMER
- Genevestigator:   P39172
- InterPro:   IPR006127
- InterPro:   IPR006128
- PRINTS:   PR00690

Pfam domain/function: PF01297 SBP_bac_9

EC number: NA

Molecular weight: Translated: 33778; Mature: 33778

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIADGVTETEVLLPDGASEH
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCCC
DYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQVTIAQLEDVKPLLMKSIHG
CCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
DDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHGKLVELMPQSRAKLDANLKDF
CCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHH
EAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFGLTPLGHFTVNPEIQPGAQRLHE
HHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCCCEEECCCCCHHHHHHHH
IRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMGTLDPLGTNIKLGKTSYSEFLSQLA
HHHHHHHHHCCEEEECCCCCHHHHHHHHCCCCEEECCCCCCCCCEEECCCHHHHHHHHHH
NQYASCLKGD
HHHHHHHCCC
>Mature Secondary Structure
MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIADGVTETEVLLPDGASEH
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCCC
DYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQVTIAQLEDVKPLLMKSIHG
CCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
DDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHGKLVELMPQSRAKLDANLKDF
CCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHH
EAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFGLTPLGHFTVNPEIQPGAQRLHE
HHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHCCCCCCCCEEECCCCCHHHHHHHH
IRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMGTLDPLGTNIKLGKTSYSEFLSQLA
HHHHHHHHHCCEEEECCCCCHHHHHHHHCCCCEEECCCCCCCCCEEECCCHHHHHHHHHH
NQYASCLKGD
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Zn (II) [Periplasm]; H2O; ATP [C]

Specific reaction: Zn (II) [Periplasm] + H2O + ATP = Zn (II) [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 9298646; 9600841; 9680209