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The map label for this gene is ttuC

Identifier: 157161264

GI number: 157161264

Start: 1899889

End: 1900974

Strand: Direct

Name: ttuC

Synonym: EcHS_A1888

Alternate gene names: 157161264

Gene position: 1899889-1900974 (Clockwise)

Preceding gene: 157161257

Following gene: 157161265

Centisome position: 40.91

GC content: 53.78

Gene sequence:

>1086_bases
ATGATGAAAACGATGCGTATTGCTGCGATACCGGGAGACGGGATTGGCAAAGAAGTCCTTCCTGAAGGGATTCGCGTGTT
ACAGGCTGCCGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACAGCCATC
ACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCG
GATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAACCTGCG
CCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATCGATTTTTACGTGGTCAGGG
AAAACACCGAAGGCGAATATTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCG
GTATTTACGCGTCGTGGTGTCGATCGCATTTTGCGTTATGCCTTCGAACTTGCGCAAAGCCGCCCACGTAAGACGCTAAC
TTCTGCCACTAAATCGAACGGTTTAGCCATCAGCATGCCGTACTGGGATGAGCGAGTGGAAGCAATGGCCGAGAATTACC
CGGAGATCCGCTGGGACAAGCAGCATATTGATATTCTCTGCGCGCGTTTTGTGATGCAGCCGGAACGCTTCGATGTGGTG
GTGGCGTCCAATTTGTTTGGCGATATCCTTTCCGATCTTGGCCCGGCCTGCACCGGCACCATTGGCATTGCCCCATCCGC
CAACCTGAATCCGGAACGCACTTTCCCGTCGCTCTTCGAGCCTGTCCACGGTTCCGCGCCGGATATCTACGGGAAAAATA
TTGCTAACCCTATCGCCACGATTTGGGCCGGGGCAATGATGCTCGATTTTCTCGGCAATGGCGATGAGCGTTTCCAGCAA
GCGCATAACGGTATTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCAC
CACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAA

Upstream 100 bases:

>100_bases
TTCGTGTTTTATGTTTCACCAGTTACGGGGATTAATTCCTTTTCAGTCAATTATAGGATGGTGATGTTGTCAATTTTGAT
GGTCAGGAAGTGAGAACCCA

Downstream 100 bases:

>100_bases
GGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTC
ACCTTTTATTGTTTCCGTTT

Product: tartrate dehydrogenase/decarboxylase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWP
DTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV
VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQ
AHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKIILR

Sequences:

>Translated_361_residues
MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWP
DTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV
VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQ
AHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKIILR
>Mature_361_residues
MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWP
DTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV
VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQ
AHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKIILR

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family

Homologues:

Organism=Homo sapiens, GI5031777, Length=358, Percent_Identity=31.5642458100559, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI4758582, Length=364, Percent_Identity=29.1208791208791, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI28178816, Length=363, Percent_Identity=27.8236914600551, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI28178838, Length=316, Percent_Identity=30.379746835443, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI28178821, Length=363, Percent_Identity=27.5482093663912, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI28178819, Length=199, Percent_Identity=28.643216080402, Blast_Score=85, Evalue=8e-17,
Organism=Escherichia coli, GI1788101, Length=361, Percent_Identity=100, Blast_Score=751, Evalue=0.0,
Organism=Escherichia coli, GI87081683, Length=369, Percent_Identity=37.1273712737127, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1787381, Length=348, Percent_Identity=27.2988505747126, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17550882, Length=362, Percent_Identity=30.939226519337, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71986051, Length=362, Percent_Identity=31.4917127071823, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17505779, Length=360, Percent_Identity=28.8888888888889, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=365, Percent_Identity=27.6712328767123, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6322097, Length=368, Percent_Identity=37.5, Blast_Score=209, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6319830, Length=367, Percent_Identity=33.5149863760218, Blast_Score=155, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6324709, Length=365, Percent_Identity=31.5068493150685, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6324291, Length=359, Percent_Identity=29.8050139275766, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24643270, Length=364, Percent_Identity=32.6923076923077, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24643268, Length=364, Percent_Identity=32.6923076923077, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24661184, Length=361, Percent_Identity=31.5789473684211, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI161078635, Length=360, Percent_Identity=29.1666666666667, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI161078633, Length=360, Percent_Identity=29.1666666666667, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24650122, Length=360, Percent_Identity=29.1666666666667, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI161078637, Length=360, Percent_Identity=29.1666666666667, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI161078639, Length=357, Percent_Identity=29.4117647058824, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI281362242, Length=357, Percent_Identity=29.1316526610644, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648872, Length=357, Percent_Identity=29.1316526610644, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI20130355, Length=363, Percent_Identity=23.9669421487603, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DMLA_ECOLI (P76251)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H64940
- RefSeq:   AP_002419.1
- RefSeq:   NP_416314.1
- ProteinModelPortal:   P76251
- SMR:   P76251
- DIP:   DIP-11800N
- IntAct:   P76251
- MINT:   MINT-1273575
- STRING:   P76251
- EnsemblBacteria:   EBESCT00000000774
- EnsemblBacteria:   EBESCT00000016215
- GeneID:   946319
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1789
- KEGG:   eco:b1800
- EchoBASE:   EB3280
- EcoGene:   EG13507
- eggNOG:   COG0473
- GeneTree:   EBGT00050000009255
- HOGENOM:   HBG518924
- OMA:   TKSNGMA
- ProtClustDB:   CLSK880210
- BioCyc:   EcoCyc:G6986-MONOMER
- BioCyc:   MetaCyc:G6986-MONOMER
- Genevestigator:   P76251
- GO:   GO:0005737
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF8
- TIGRFAMs:   TIGR02089

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.83

Molecular weight: Translated: 40315; Mature: 40315

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPD
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCH
DWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS
CCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
RPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV
CCHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHCEE
VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT
EHHHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHH
IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKIIL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHC
R
C
>Mature Secondary Structure
MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPD
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCH
DWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS
CCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
RPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV
CCHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHCEE
VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT
EHHHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHH
IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKIIL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503