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The map label for this gene is gadW [H]

Identifier: 157161033

GI number: 157161033

Start: 1670401

End: 1671114

Strand: Reverse

Name: gadW [H]

Synonym: EcHS_A1647

Alternate gene names: 157161033

Gene position: 1671114-1670401 (Counterclockwise)

Preceding gene: 157161034

Following gene: 157161032

Centisome position: 35.99

GC content: 33.33

Gene sequence:

>714_bases
ATGCCATACATATGTTCTATCATTTTGGTGTTGAACTCGTTTGGTGTCCGAATTGGTAAAGAAGATATTTTGTTTAAAAA
AGGAAATGCTGTCCTCATTGAATACAATTTAAAAGATCTTTTTTCGTCAAATACAGATCATCTAATGATCGTAGATATTG
AGGAGAAAACAGTTAATGATTTCTTTAAAAACTACACACTCTCACCTTTTTCTGTAAGAAGGTTTTATCCGTCATACTTG
ATAGTAGAATGTGAAGATTTTTCATTGTTAAGGAACTTGGTTGCATGCTTGAATTGTGATAGCAAAACCGTGGATGTAGT
TAGAAATCAAATATCACTTGCATGTCTTGCTGTCTTATCTTCAGAGAAAATAGTGCAAAGTTTTTTGTTCGGAAGTCTTA
ATAGCTTAGGTTGTAAAGTTAAGGCTATTATTCACTCGGATATCTCAGCGTCATGGAGACTTTGTGATATATCTTCAAGA
TTATATATGAGCGAGAGTTTGTTAAAAAGAAAATTAAAAGACGAAGGATTATCGTTTAGTAAATTAATTCTTGAAGAACG
AATGATGATGGCGCAAAGATTATTGATCTACAGTAATCATACTGTCGGCAAGGTGGCGGGAATATGCGGGTATGACAATG
CATCATATTTTGTAAGTGTTTTTAGAGGATATTTTGGTGTGCCTCCGCACCAATATTTATCAAGATTTTCTTAG

Upstream 100 bases:

>100_bases
AGTTGTGATGCAATGAGATTTTCCTTATTGTTGAACTGGCGAATATTGATTTTCCACCTATACTTACCTAGTGTAACCCC
AATGATATCAGGTGGATAAT

Downstream 100 bases:

>100_bases
AAGATATGATGTGATGGCATTTTATAAAGTTATTTTTTGAAGTGTAAGATTATACGATATTTTTAGCTACGCCAGAATAA
TTGCTGGCGTTTTTCTTTTT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MPYICSIILVLNSFGVRIGKEDILFKKGNAVLIEYNLKDLFSSNTDHLMIVDIEEKTVNDFFKNYTLSPFSVRRFYPSYL
IVECEDFSLLRNLVACLNCDSKTVDVVRNQISLACLAVLSSEKIVQSFLFGSLNSLGCKVKAIIHSDISASWRLCDISSR
LYMSESLLKRKLKDEGLSFSKLILEERMMMAQRLLIYSNHTVGKVAGICGYDNASYFVSVFRGYFGVPPHQYLSRFS

Sequences:

>Translated_237_residues
MPYICSIILVLNSFGVRIGKEDILFKKGNAVLIEYNLKDLFSSNTDHLMIVDIEEKTVNDFFKNYTLSPFSVRRFYPSYL
IVECEDFSLLRNLVACLNCDSKTVDVVRNQISLACLAVLSSEKIVQSFLFGSLNSLGCKVKAIIHSDISASWRLCDISSR
LYMSESLLKRKLKDEGLSFSKLILEERMMMAQRLLIYSNHTVGKVAGICGYDNASYFVSVFRGYFGVPPHQYLSRFS
>Mature_236_residues
PYICSIILVLNSFGVRIGKEDILFKKGNAVLIEYNLKDLFSSNTDHLMIVDIEEKTVNDFFKNYTLSPFSVRRFYPSYLI
VECEDFSLLRNLVACLNCDSKTVDVVRNQISLACLAVLSSEKIVQSFLFGSLNSLGCKVKAIIHSDISASWRLCDISSRL
YMSESLLKRKLKDEGLSFSKLILEERMMMAQRLLIYSNHTVGKVAGICGYDNASYFVSVFRGYFGVPPHQYLSRFS

Specific function: Depending on the conditions (growth phase and medium), acts as a positive or negative regulator of gadA and gadBC. Repression occurs directly or via the repression of the expression of gadX. Activation occurs directly by the binding of gadW to the gadA an

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789932, Length=233, Percent_Identity=33.9055793991416, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1786776, Length=238, Percent_Identity=30.2521008403361, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787776, Length=229, Percent_Identity=28.82096069869, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790557, Length=241, Percent_Identity=30.2904564315353, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789933, Length=101, Percent_Identity=40.5940594059406, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1786778, Length=218, Percent_Identity=31.1926605504587, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 26947; Mature: 26816

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYICSIILVLNSFGVRIGKEDILFKKGNAVLIEYNLKDLFSSNTDHLMIVDIEEKTVND
CCHHHHHHHHHHHHCCEECCCCEEEECCCEEEEEECHHHHHCCCCCEEEEEEECHHHHHH
FFKNYTLSPFSVRRFYPSYLIVECEDFSLLRNLVACLNCDSKTVDVVRNQISLACLAVLS
HHHCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SEKIVQSFLFGSLNSLGCKVKAIIHSDISASWRLCDISSRLYMSESLLKRKLKDEGLSFS
HHHHHHHHHHHCHHHCCCEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCHH
KLILEERMMMAQRLLIYSNHTVGKVAGICGYDNASYFVSVFRGYFGVPPHQYLSRFS
HHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCCHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
PYICSIILVLNSFGVRIGKEDILFKKGNAVLIEYNLKDLFSSNTDHLMIVDIEEKTVND
CHHHHHHHHHHHHCCEECCCCEEEECCCEEEEEECHHHHHCCCCCEEEEEEECHHHHHH
FFKNYTLSPFSVRRFYPSYLIVECEDFSLLRNLVACLNCDSKTVDVVRNQISLACLAVLS
HHHCCCCCCHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SEKIVQSFLFGSLNSLGCKVKAIIHSDISASWRLCDISSRLYMSESLLKRKLKDEGLSFS
HHHHHHHHHHHCHHHCCCEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCHH
KLILEERMMMAQRLLIYSNHTVGKVAGICGYDNASYFVSVFRGYFGVPPHQYLSRFS
HHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCCHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]