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The map label for this gene is ydeH [H]

Identifier: 157161011

GI number: 157161011

Start: 1646027

End: 1646917

Strand: Reverse

Name: ydeH [H]

Synonym: EcHS_A1625

Alternate gene names: 157161011

Gene position: 1646917-1646027 (Counterclockwise)

Preceding gene: 157161012

Following gene: 157161008

Centisome position: 35.47

GC content: 44.78

Gene sequence:

>891_bases
ATGATCAAGAAGACAACGGAAATTGATGCCATCTTGTTAAATCTCAATAAGGCTATCGATGCCCACTACCAGTGGCTGGT
GAGTATGTTTCACAGCGTGGTCGCGAGAGATGCCAGTAAGCCAGAAATAACGGATAACCATTCTTATGGACTGTGCCAGT
TTGGTCGGTGGATTGATCATCTGGGGCCACTCGATAACGATGAATTACCTTACGTTCGGCTAATGGATTCTGCCCATCAA
CATATGCATAACTGTGGTCGGGAATTAATGCTGGCTATTGTTGAAAATCACTGGCAGGACGCGCATTTCGACACCTTTCA
GGAGGGGTTGTTTTCTTTTACTGCGGCATTAACCGATTACAAAATTTATTTGCTGACGATCCGTAGCAATATGGATGTTT
TGACGGGATTGCCGGGGCGTCGGGTTCTTGATGAATCCTTTGATCATCAGTTACGCAACGCTGAGCCTCTGAATCTTTAT
TTAATGTTGTTGGATATTGACCGATTTAAATTGGTTAATGATACCTACGGGCATTTAATCGGCGATGTAGTATTACGCAC
CCTGGCAACTTACTTAGCCAGTTGGACGCGTGATTACGAAACGGTTTATCGCTACGGGGGCGAAGAATTTATCATTATTG
TCAAAGCGGCTAATGATGAAGAAGCATGTCGTGCAGGTGTCAGAATTTGCCAGTTAGTCGATAACCATGCCATCACACAT
TCTGAAGGGCATAACAACATTACCGTGACAGCAGGTGTCAGTCGCGCATTTCCTGAAGAGCCTCTGGATGTGGTCATTGG
AAGAGCAGACCGGGCAATGTATGAGGGTAAGCAAACCGGAAGAAACCGCTGCATGTTTATTGACGAACAAAATGTGATTA
ACCGAGTTTAA

Upstream 100 bases:

>100_bases
AGATGACTCATAACTTCGGATTTCTATGAAAATATTGACGTAATTTTGCTTTTCTCTCGTTAGAATAGCGCGCACAAGGA
ACTGTGAAAAAGGAGTGGCA

Downstream 100 bases:

>100_bases
GCTCCGTTTAACATTCATTGAGAAAACTGATGCTACTGTGTCAACGATCCGTTATCAGTGCAGGAAAATGCCTGTTAGCG
TAAAAGCAAAACACAAATCT

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQ
HMHNCGRELMLAIVENHWQDAHFDTFQEGLFSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
SEGHNNITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV

Sequences:

>Translated_296_residues
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQ
HMHNCGRELMLAIVENHWQDAHFDTFQEGLFSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
SEGHNNITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV
>Mature_296_residues
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQ
HMHNCGRELMLAIVENHWQDAHFDTFQEGLFSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
SEGHNNITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV

Specific function: A diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returnes to normal when residues 206-207 are both mutated to Ala. Overexpression also leads to a reduction in flagellar abundance and a 20-fold increase in c-di- GMP

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787816, Length=296, Percent_Identity=98.9864864864865, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=34.1614906832298, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081881, Length=160, Percent_Identity=35.625, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787056, Length=191, Percent_Identity=31.9371727748691, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=30.5555555555556, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788381, Length=174, Percent_Identity=31.0344827586207, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=29.8780487804878, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=31.0975609756098, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082007, Length=130, Percent_Identity=35.3846153846154, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=106, Percent_Identity=35.8490566037736, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788956, Length=151, Percent_Identity=29.1390728476821, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33929; Mature: 33929

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDH
CCCCCHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAHFDTFQEGLFSFTAALTDY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
KIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLYLMLLDIDRFKLVNDTYGHLI
EEEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHH
GDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEC
SEGHNNITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV
CCCCCEEEEEECCHHCCCCCCHHHHHCCHHHHHHCCHHCCCCCEEEEECHHHHCCC
>Mature Secondary Structure
MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEITDNHSYGLCQFGRWIDH
CCCCCHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAHFDTFQEGLFSFTAALTDY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
KIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLYLMLLDIDRFKLVNDTYGHLI
EEEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHH
GDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEC
SEGHNNITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV
CCCCCEEEEEECCHHCCCCCCHHHHHCCHHHHHHCCHHCCCCCEEEEECHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383113; 9097039; 9278503 [H]