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The map label for this gene is cof

Identifier: 157159973

GI number: 157159973

Start: 533477

End: 534295

Strand: Direct

Name: cof

Synonym: EcHS_A0523

Alternate gene names: 157159973

Gene position: 533477-534295 (Clockwise)

Preceding gene: 157159970

Following gene: 157159974

Centisome position: 11.49

GC content: 51.28

Gene sequence:

>819_bases
ATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCTGACCATCATTTAGGTGAGAAAACCCTCTCTAC
TTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCG
GGGCGCTATCGCTGGATGCATATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGT
GATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA
CGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCG
ATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATC
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGA
TGAACGATCGCGAAATGTTAGGCAGCGTCGGTAGCGGATTTATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTC
CCGCATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCT
ACCTTATTCCCCCGAATAA

Upstream 100 bases:

>100_bases
TATAAACCCGGAACAATATTATTTCACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGT
GTCACAGAGTGGAGAAAGAA

Downstream 100 bases:

>100_bases
CGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCG
CACAGAATAAATTGTCGTGA

Product: hydrolase Cof

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDAYLITGNGTRVHSLEGELLHR
DDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAEL
PHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE

Sequences:

>Translated_272_residues
MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDAYLITGNGTRVHSLEGELLHR
DDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAEL
PHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE
>Mature_271_residues
ARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRD
DLPADVAELVLYQQWDTRASMHIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQIQ
LYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAELP
HLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P)

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family

Homologues:

Organism=Escherichia coli, GI87081741, Length=272, Percent_Identity=99.6323529411765, Blast_Score=559, Evalue=1e-161,
Organism=Escherichia coli, GI48994981, Length=260, Percent_Identity=38.8461538461538, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI2367265, Length=243, Percent_Identity=26.3374485596708, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COF_ECO24 (A7ZIK4)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001461633.1
- ProteinModelPortal:   A7ZIK4
- SMR:   A7ZIK4
- STRING:   A7ZIK4
- EnsemblBacteria:   EBESCT00000021154
- GeneID:   5590290
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0482
- eggNOG:   COG0561
- GeneTree:   EBGT00050000009623
- HOGENOM:   HBG728500
- OMA:   SAMDCLE
- ProtClustDB:   PRK15126
- BioCyc:   ECOL331111:ECE24377A_0482-MONOMER
- HAMAP:   MF_01847
- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR00099
- TIGRFAMs:   TIGR01484

Pfam domain/function: PF08282 Hydrolase_3; SSF56784 SSF56784

EC number: NA

Molecular weight: Translated: 30329; Mature: 30198

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01228 COF_1; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDA
CCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCE
YLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKEIPALL
EEEECCCCEEEECCCCEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEECCHHHHHHH
QAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQIQLYEALGERAHLCFSATDCL
HHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHEEEECHHHHH
EVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAEL
HHEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHCCCCCEEECCCCHHHHHCC
PHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE
CCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDA
CCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCE
YLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKEIPALL
EEEECCCCEEEECCCCEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEECCHHHHHHH
QAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQIQLYEALGERAHLCFSATDCL
HHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHEEEECHHHHH
EVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAEL
HHEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHCCCCCEEECCCCHHHHHCC
PHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE
CCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA