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The map label for this gene is fghA2 [H]

Identifier: 157159872

GI number: 157159872

Start: 442819

End: 443652

Strand: Reverse

Name: fghA2 [H]

Synonym: EcHS_A0420

Alternate gene names: 157159872

Gene position: 443652-442819 (Counterclockwise)

Preceding gene: 157159873

Following gene: 157159862

Centisome position: 9.55

GC content: 49.16

Gene sequence:

>834_bases
ATGGAACTCATTGAAAAACATGCCAGCTTTGGCGGCTGGCAAAATGTGTATCGGCATTATTCCCAATCACTGAAATGTGA
AATGAATGTCGGCGTCTATCTCCCACCAAAAGCCGCGAATGAAAAATTGCCGGTGCTGTACTGGCTTTCGGGCCTGACCT
GCAACGAGCAGAATTTCATTACTAAATCGGGGATGCAGCGTTACGCGGCTGAACACAACATTATTGTTGTTGCGCCGGAC
ACCAGTCCGCGAGGCAGTCATGTCGCAGATGCCGACCGTTACGATCTCGGGCAAGGTGCCGGGTTTTACCTGAACGCAAC
GCAAGCGCCGTGGAATGAACATTACAAAATGTATGACTATATCCGCAACGAGCTGCCGGATTTAGTGATGCAACATTTTC
CGGCAACGACCAGAAAGTCTATCTCTGGTCATTCTATGGGCGGGCTGGGCGCGCTGGTGCTGGCGTTACGTAACCCAGAT
GAATATGTCAGCGTCTCGGCGTTTTCGCCCATTGTCTCCCCATCGCAAGCACCGTGGGGACAGCAAGCCTTTGCCGCATA
TCTTGGTGAAAATAAAGATGCCTGGTTGGATTACGATCCGGTGAGTCTTATTTCACAAGGTCAACGCGTTGCGGAAATCA
TGGTTGATCAGGGGTTGAGTGATGATTTTTACGCGGAACAGCTGCGGACTCCAAATCTTGAAAAGATCTGCCAGGAGATG
AATATCAAGACGTTAATCCGTTATCACGAGGGTTACGACCACAGCTATTATTTTGTCTCCAGTTTTATTGGCGAGCATAT
TGCCTACCACGCCAATAAACTGAATATGCGTTGA

Upstream 100 bases:

>100_bases
GTTACTGATTTCCCGCAGGTATACCCCGTCCACTTCAGACGGGGTTCTTAATACTCTCCCTGGGCAGCCGTCCGGGGGAT
TAACCGTGAGATAATGACTG

Downstream 100 bases:

>100_bases
TAATAGTGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACACTTCGCCCGTAAACCGTAGATACTGTTATG
GCCTGCAAGTTTTTTCCTGG

Product: S-formylglutathione hydrolase

Products: NA

Alternate protein names: FGH [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLPVLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPD
TSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
EYVSVSAFSPIVSPSQAPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR

Sequences:

>Translated_277_residues
MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLPVLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPD
TSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
EYVSVSAFSPIVSPSQAPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR
>Mature_277_residues
MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLPVLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPD
TSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
EYVSVSAFSPIVSPSQAPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR

Specific function: Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate [H]

COG id: COG0627

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the esterase D family [H]

Homologues:

Organism=Homo sapiens, GI33413400, Length=280, Percent_Identity=49.6428571428571, Blast_Score=289, Evalue=2e-78,
Organism=Escherichia coli, GI1786551, Length=277, Percent_Identity=97.4729241877256, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI1788477, Length=276, Percent_Identity=53.9855072463768, Blast_Score=313, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI17510185, Length=280, Percent_Identity=45.7142857142857, Blast_Score=249, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6322393, Length=298, Percent_Identity=40.9395973154362, Blast_Score=214, Evalue=9e-57,
Organism=Drosophila melanogaster, GI45551932, Length=281, Percent_Identity=50.5338078291815, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24648347, Length=281, Percent_Identity=50.5338078291815, Blast_Score=285, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000801
- InterPro:   IPR014186 [H]

Pfam domain/function: PF00756 Esterase [H]

EC number: =3.1.2.12 [H]

Molecular weight: Translated: 31372; Mature: 31372

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLPVLYWLSGLTCNEQNFI
CCHHHHHHCCCCHHHHHHHHHHCCEEEECCCEEECCCCCCCCCCEEEEECCCEECCCCCH
TKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDY
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
IRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPDEYVSVSAFSPIVSPSQAPWG
HHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHEEEEECCCCCEEEEHHCCCCCCCCCCCCC
QQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
HHHHHHHHCCCCCCEECCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHC
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLPVLYWLSGLTCNEQNFI
CCHHHHHHCCCCHHHHHHHHHHCCEEEECCCEEECCCCCCCCCCEEEEECCCEECCCCCH
TKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGFYLNATQAPWNEHYKMYDY
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
IRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPDEYVSVSAFSPIVSPSQAPWG
HHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHEEEEECCCCCEEEEHHCCCCCCCCCCCCC
QQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQGLSDDFYAEQLRTPNLEKICQEM
HHHHHHHHCCCCCCEECCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHC
NIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNMR
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA