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The map label for this gene is 157159687

Identifier: 157159687

GI number: 157159687

Start: 250417

End: 251748

Strand: Reverse

Name: 157159687

Synonym: EcHS_A0231

Alternate gene names: NA

Gene position: 251748-250417 (Counterclockwise)

Preceding gene: 157159688

Following gene: 157159686

Centisome position: 5.42

GC content: 55.26

Gene sequence:

>1332_bases
ATGGCAACCACCCGCAACAAGGTGATGTGGCAGGAAGGGATGCTGATGCGTCCACACCATTTCCAGCAGCAGCAGCGTTA
CAACGACTACCTGGATAACCAGCGTTTCCGGGCCATGAATAATTTATCCTGGGGATTTACCGAACTCACTCTCAACAATG
AACTGCTGGCACAGGGTAAGATCATGATTGACAGCGCGTCAGGCACACTGCCCGACGGCACCGTCTTTTCTATCCCCGAC
CAGGACGCACTGCCCGATCCGCTGCACCCGCAACATTTTCCGGACGAGAGAAGCCGCAATATCTACCTCGCTCTGCCGGT
CGCCAGTGATGTGAGAAATGAAATCAGCGACGGGCGGCGAATCGGGCGTTACCGGCTGAATTATGCCGATGTCCGGGATT
TGCATTCAGAAGAAGGTGACACGCGAACACTGACGCTGGGACAACTGACGCCGCGCATTATGAGCGGTGCAGAAGATATG
AGCGCCTATATTACGCTCCCGCTTTGCCGTATCAGTGATCGCCATGCCGACGGCTCCCTGACGCTGGATGACGATTTTAT
CCCCTCCTGCCAGAATATTCAGGTCAGTAAGAAACTGCGTGTTTATCTCAAAGAGGTACAGGGGGCCATTGGCGGACGGG
CAAGCGATCTGGCAAACCGCATTGGCTCTCCGGCGCAGAGCGGCATCGCGGATGTGGCGGAATTTATGATGTTGCAGTTA
CTTAACCGTAACCAGACCCGGTTTACCCATCGCGCTCGTCGATCCCAGCTCCACCCGGAAGATTTCTACCTTGATCTTGC
CGGGTTGCTGGGTGAACTGATGACCTTTACCGAGCCGTCGCGCCTGCCCTGCCCGCTTGATGTGTATGATCATCATGACC
TGACCAAAACATTTAAAACGCTGTTACCGGAAGTCAAACGGGCGCTGCATACCGTACTGTCGCCAAGAGCCGTTAATCTG
CCGCTGCATCTGCGTGACGGTATCTGGCAGGCCGATATCCATGACACAGAACTGCTGCAATCTGCCACCTTTGTGCTGGC
TGTGGCGGCAAACATGCCGGTCGATCAGATCCAGCGCCAGTTTATCCAGCAGTCGAAAATTTCCTCGCCGGAAAAAATCC
GCAATATGGTCAGTGTGCAGATACCCGGCATTCCATTGCGTGCCCTGATGGTGGCTCCCCGCCAGCTTCCTTACCATTCC
GGGTTCAGCTATTTCGAACTCGACAAGAGCGGACAGGCCTGGACAGAAATGGCTGCCGCCGGGGCCGTTGCACTGCATGT
ATCCGGCAGTTTCCCGGATCTGAACATGCAACTGTGGGCGATAAGAGGGTAA

Upstream 100 bases:

>100_bases
GGAAACAAATCGAGACGGTGGAAGGAACCGGCCACCATTACCGCCTGCTGGTGCATGTACGTCAAAGCAGCATCGAAATG
AAAAAAGAGGATGAATAATT

Downstream 100 bases:

>100_bases
GACGATGGATGAGGGGTCACTGTCTCTGCCACCATTTACCGGATACGACGAAAAAAGCCAGCGCGACTATCATCTCGCGC
TTCGTGGCAACAGCCTGAAT

Product: hypothetical protein

Products: NA

Alternate protein names: Type VI Secretion Protein Family; Protein ImpJ/VasE; Conserved Protein; Replication/Virulence Associated Protein; Type VI Secretion System Protein EvpM; Protein ImpJ; Type VI Secretion System Protein; Type VI Secretion System Protein ImpJ; Type VI Secretion; Type VI Secretion System Core Protein; T6ss M; Type VI Secretion Protein VasE; Temperature-Dependent Protein Secretion ImpJ

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNNLSWGFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPD
QDALPDPLHPQHFPDERSRNIYLALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLANRIGSPAQSGIADVAEFMMLQL
LNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTFTEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNL
PLHLRDGIWQADIHDTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVAPRQLPYHS
GFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG

Sequences:

>Translated_443_residues
MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNNLSWGFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPD
QDALPDPLHPQHFPDERSRNIYLALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLANRIGSPAQSGIADVAEFMMLQL
LNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTFTEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNL
PLHLRDGIWQADIHDTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVAPRQLPYHS
GFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG
>Mature_442_residues
ATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNNLSWGFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQ
DALPDPLHPQHFPDERSRNIYLALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDMS
AYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLANRIGSPAQSGIADVAEFMMLQLL
NRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTFTEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLP
LHLRDGIWQADIHDTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVAPRQLPYHSG
FSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG

Specific function: Unknown

COG id: COG3522

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 49993; Mature: 49861

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNNLSWGFTELTLNNELLAQGK
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEECCHHHCCCE
IMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIYLALPVASDVRNEISDGRR
EEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCHH
IGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDMSAYITLPLCRISDRHADGSL
HCCEECCHHHHHHHHCCCCCEEEEEHHHCCHHHHCCHHHCEEEEEEEEEECCCCCCCCCE
TLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLANRIGSPAQSGIADVAEFMMLQL
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHH
LNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTFTEPSRLPCPLDVYDHHDLTKTFKT
HCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIHDTELLQSATFVLAVAANMPVDQIQRQ
HHHHHHHHHHHHHCCCEECCCEEECCCCEECCCCHHHHHHHHHEEEEEECCCCHHHHHHH
FIQQSKISSPEKIRNMVSVQIPGIPLRALMVAPRQLPYHSGFSYFELDKSGQAWTEMAAA
HHHHHCCCCHHHHHHHEEEECCCCCHHHHHHCCHHCCCCCCCCEEEECCCCCHHHHHHHC
GAVALHVSGSFPDLNMQLWAIRG
CEEEEEECCCCCCCCEEEEEECC
>Mature Secondary Structure 
ATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNNLSWGFTELTLNNELLAQGK
CCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEECCHHHCCCE
IMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIYLALPVASDVRNEISDGRR
EEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCHH
IGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDMSAYITLPLCRISDRHADGSL
HCCEECCHHHHHHHHCCCCCEEEEEHHHCCHHHHCCHHHCEEEEEEEEEECCCCCCCCCE
TLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLANRIGSPAQSGIADVAEFMMLQL
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHH
LNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTFTEPSRLPCPLDVYDHHDLTKTFKT
HCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIHDTELLQSATFVLAVAANMPVDQIQRQ
HHHHHHHHHHHHHCCCEECCCEEECCCCEECCCCHHHHHHHHHEEEEEECCCCHHHHHHH
FIQQSKISSPEKIRNMVSVQIPGIPLRALMVAPRQLPYHSGFSYFELDKSGQAWTEMAAA
HHHHHCCCCHHHHHHHEEEECCCCCHHHHHHCCHHCCCCCCCCEEEECCCCCHHHHHHHC
GAVALHVSGSFPDLNMQLWAIRG
CEEEEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA